Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source

Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance

(2021) Andrew Hammond et al.   PLoS Genetics

Converting endogenous genes of the malaria mosquito into simple non-autonomous gene drives for population replacement

(2021) Astrid Hoermann et al.   eLife

CRISPR-mediated knock-in of transgenes into the malaria vector Anopheles funestus

(2021) Charlotte Quinn et al.   G3-Genes Genomes Genetics

CRISPR/Cas9 modified An. gambiae carrying kdr mutation L1014F functionally validate its contribution in insecticide resistance and combined effect with metabolic enzymes

(2021) Linda Grigoraki et al.   PLoS Genetics

Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation

(2021) Silke Fuchs et al.   PLoS Genetics

CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish

(2020) Haipeng Bai et al.   BMC GENOMICS

Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs

(2020) Samuel E. Champer et al.   Science Advances

Next-generation gene drive for population modification of the malaria vector mosquito, Anopheles gambiae

(2020) Rebeca Carballar-Lejarazú et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular safeguarding of CRISPR gene drive experiments

(2019) Jackson Champer et al.   eLife

CRISPResso2 provides accurate and rapid genome editing sequence analysis

(2019) Kendell Clement et al.   NATURE BIOTECHNOLOGY

The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti

(2019) Benjamin J Matthews et al.   eLife

An efficient gene knock-in strategy using 5′-modified double-stranded DNA donors with short homology arms

(2019) Yi Yu et al.   Nature Chemical Biology

Experimental population modification of the malaria vector mosquito, Anopheles stephensi

(2019) Thai Binh Pham et al.   PLoS Genetics

Resistance of Aedes aegypti (Diptera: Culicidae) Populations to Deltamethrin, Permethrin, and Temephos in Cambodia

(2018) Sébastien Boyer et al.   Asia-Pacific Journal of Public Health

Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

(2018) Denise G. Lanza et al.   BMC BIOLOGY

A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

(2018) Kyros Kyrou et al.   NATURE BIOTECHNOLOGY

Efficient single-copy HDR by 5’ modified long dsDNA donors

(2018) Jose Arturo Gutierrez-Triana et al.   eLife

The influence of eukaryotic chromatin state on CRISPR–Cas9 editing efficiencies

(2018) Sebald AN Verkuijl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency

(2017) Kristopher Torp Jensen et al.   FEBS LETTERS

Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector,Aedes aegypti

(2017) Ming Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations

(2017) Jackson Champer et al.   PLoS Genetics

The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito

(2017) Andrew M. Hammond et al.   PLoS Genetics

Optimizing the DNA Donor Template for Homology-Directed Repair of Double-Strand Breaks

(2017) Fei Song et al.   Molecular Therapy-Nucleic Acids

The global burden of dengue: an analysis from the Global Burden of Disease Study 2013

(2016) Jeffrey D Stanaway et al.   LANCET INFECTIOUS DISEASES

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells

(2015) Michael Ratz et al.   Scientific Reports

Genome Engineering with CRISPR-Cas9 in the Mosquito Aedes aegypti

(2015) Kathryn E. Kistler et al.   Cell Reports

Trimmomatic: a flexible trimmer for Illumina sequence data

(2014) Anthony M. Bolger et al.   BIOINFORMATICS

Adenoviral vector DNA for accurate genome editing with engineered nucleases

(2014) Maarten Holkers et al.   NATURE METHODS

Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System

(2013) Andrew R. Bassett et al.   Cell Reports

Double-Strand Break Repair Assays Determine Pathway Choice and Structure of Gene Conversion Events in Drosophila melanogaster

(2013) Anthony T. Do et al.   G3-Genes Genomes Genetics