One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii
Published 2022 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii
Authors
Keywords
-
Journal
ACS Synthetic Biology
Volume 11, Issue 2, Pages 644-654
Publisher
American Chemical Society (ACS)
Online
2022-01-31
DOI
10.1021/acssynbio.1c00302
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Robust and flexible platform for directed evolution of yeast genetic switches
- (2021) Masahiro Tominaga et al. Nature Communications
- Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris
- (2021) Peng Cai et al. NUCLEIC ACIDS RESEARCH
- Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression
- (2020) Yoichiro Ito et al. NUCLEIC ACIDS RESEARCH
- Pichia pastoris as a versatile cell factory for the production of industrial enzymes and chemicals: current status and future perspectives
- (2019) Taicheng Zhu et al. Biotechnology Journal
- Current advances in engineering tools for Pichia pastoris
- (2019) Jasmin E Fischer et al. CURRENT OPINION IN BIOTECHNOLOGY
- A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA
- (2018) Yasuyuki Nakamura et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris
- (2018) Thomas Vogl et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
- (2017) Kate E. Royle et al. Scientific Reports
- A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production
- (2017) Jan-Philipp Schwarzhans et al. Frontiers in Microbiology
- Refined Pichia pastoris reference genome sequence
- (2016) Lukas Sturmberger et al. JOURNAL OF BIOTECHNOLOGY
- Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications
- (2016) Andrea Camattari et al. Microbial Cell Factories
- Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing
- (2016) Jan-Philipp Schwarzhans et al. Scientific Reports
- High-throughput plasmid construction using homologous recombination in yeast: its mechanisms and application to protein production for X-ray crystallography
- (2015) Kimihiko Mizutani BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris
- (2015) Kartik A. Shah et al. BIOTECHNOLOGY AND BIOENGINEERING
- A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris
- (2015) Thomas Vogl et al. ACS Synthetic Biology
- CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae
- (2015) Tadas Jakočiu̅nas et al. ACS Synthetic Biology
- Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production
- (2014) Mudassar Ahmad et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease inSaccharomyces cerevisiae
- (2013) Niels G.A. Kuijpers et al. FEMS YEAST RESEARCH
- An autonomously replicating sequence for use in a wide range of budding yeasts
- (2013) Ivan Liachko et al. FEMS YEAST RESEARCH
- Pichia pastoris: protein production host and model organism for biomedical research
- (2013) Brigitte Gasser et al. Future Microbiology
- The Fate of Linear DNA in Saccharomyces cerevisiae and Candida glabrata: The Role of Homologous and Non-Homologous End Joining
- (2013) Mary W. Corrigan et al. PLoS One
- Synthetic Core Promoters for Pichia pastoris
- (2013) Thomas Vogl et al. ACS Synthetic Biology
- Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology
- (2012) Laura Näätsaari et al. PLoS One
- High-quality genome sequence of Pichia pastoris CBS7435
- (2011) Andreas Küberl et al. JOURNAL OF BIOTECHNOLOGY
- High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins
- (2010) Kimihiko Mizutani et al. PROTEIN EXPRESSION AND PURIFICATION
- Genome sequence of the recombinant protein production host Pichia pastoris
- (2009) Kristof De Schutter et al. NATURE BIOTECHNOLOGY
- Expression of GFP usingPichia pastorisvectors with zeocin or G-418 sulphate as the primary selectable marker
- (2009) Theo Papakonstantinou et al. YEAST
- One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
- (2008) D. G. Gibson et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome
- (2008) D. G. Gibson et al. SCIENCE
Discover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversationAsk a Question. Answer a Question.
Quickly pose questions to the entire community. Debate answers and get clarity on the most important issues facing researchers.
Get Started