Pichia pastoris: protein production host and model organism for biomedical research

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions

(2013) Marc Carnicer et al.   Microbial Cell Factories

Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation

(2012) Martin Pfeffer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Recent developments in yeast cell surface display toward extended applications in biotechnology

(2012) Tsutomu Tanaka et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in-silico evaluation of their potentials

(2012) Luis Caspeta et al.   BMC Systems Biology

Tracing Putative Trafficking of the Glycolytic Enzyme Enolase via SNARE-Driven Unconventional Secretion

(2012) Natsuko Miura et al.   EUKARYOTIC CELL

Oxidative protein folding and unfolded protein response elicit differing redox regulation in endoplasmic reticulum and cytosol of yeast

(2012) Marizela Delic et al.   FREE RADICAL BIOLOGY AND MEDICINE

Engineering Aggregation Resistance in IgG by Two Independent Mechanisms: Lessons from Comparison of Pichia pastoris and Mammalian Cell Expression

(2012) Jonas V. Schaefer et al.   JOURNAL OF MOLECULAR BIOLOGY

U13C cell extract of Pichia pastoris - a powerful tool for evaluation of sample preparation in metabolomics

(2012) Stefan Neubauer et al.   JOURNAL OF SEPARATION SCIENCE

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

(2012) Joel Jordà et al.   Microbial Cell Factories

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

(2012) Ana Leticia Vanz et al.   Microbial Cell Factories

Sec12 Binds to Sec16 at Transitional ER Sites

(2012) Elisabeth A. Montegna et al.   PLoS One

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

(2012) Kerry Routenberg Love et al.   PLoS One

Protein Folding and Modification in the Mammalian Endoplasmic Reticulum

(2011) Ineke Braakman et al.   Annual Review of Biochemistry

Generation and Screening of Pichia pastoris Strains with Enhanced Protein Production by Use of Microengraving

(2011) Vasiliki Panagiotou et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

GAPPromoter Library for Fine-Tuning of Gene Expression in Pichia pastoris

(2011) Xiulin Qin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Novel Yeast Bioassay for High-Throughput Screening of Matrix Metalloproteinase Inhibitors

(2011) Bjoern Diehl et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Hydrophobin signal sequence mediates efficient secretion of recombinant proteins in Pichia pastoris

(2011) Kirsten Kottmeier et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A proteomic analysis of the Pichia pastoris secretome in methanol-induced cultures

(2011) Chung-Jr Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Protein secretion in Pichia pastoris and advances in protein production

(2011) Leonardo M. Damasceno et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

(2011) Kristin Baumann et al.   BMC GENOMICS

Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems

(2011) Jeffrey L Brodsky et al.   CURRENT OPINION IN CELL BIOLOGY

Recombinant expression of ShPI-1A, a non-specific BPTI-Kunitz-type inhibitor, and its protection effect on proteolytic degradation of recombinant human miniproinsulin expressed in Pichia pastoris

(2011) Dayrom F. Gil et al.   FEMS YEAST RESEARCH

High-quality genome sequence of Pichia pastoris CBS7435

(2011) Andreas Küberl et al.   JOURNAL OF BIOTECHNOLOGY

Peroxisome assembly: matrix and membrane protein biogenesis

(2011) Changle Ma et al.   JOURNAL OF CELL BIOLOGY

Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

(2011) Martin Pfeffer et al.   Microbial Cell Factories

High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection

(2011) Hakan Dortay et al.   PLoS One

Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

(2011) Graham Whyteside et al.   PLoS One

Catabolite Repression of Aox in Pichia pastoris Is Dependent on Hexose Transporter PpHxt1 and Pexophagy

(2010) P. Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of glycosylation in yeast and other fungi: current state and perspectives

(2010) Karen De Pourcq et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production

(2010) Jan Heyland et al.   BIOTECHNOLOGY AND BIOENGINEERING

Systems metabolic engineering: Genome-scale models and beyond

(2010) John Blazeck et al.   Biotechnology Journal

Genome-scale metabolic model of methylotrophic yeastPichia pastorisand its use forin silicoanalysis of heterologous protein production

(2010) Seung Bum Sohn et al.   Biotechnology Journal

The response to unfolded protein is involved in osmotolerance of Pichia pastoris

(2010) Martin Dragosits et al.   BMC GENOMICS

A multi-level study of recombinant Pichia pastoris in different oxygen conditions

(2010) Kristin Baumann et al.   BMC Systems Biology

Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts

(2010) Marizela Delic et al.   FEMS MICROBIOLOGY LETTERS

Recombinant protein vaccines against the asexual blood-stages ofPlasmodium falciparum

(2010) Robin F. Anders et al.   Human vaccines & immunotherapeutics

Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production

(2010) Gerhard Stadlmayr et al.   JOURNAL OF BIOTECHNOLOGY

Unconventional secretion ofPichia pastorisAcb1 is dependent on GRASP protein, peroxisomal functions, and autophagosome formation

(2010) Ravi Manjithaya et al.   JOURNAL OF CELL BIOLOGY

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

(2010) Gavin C. Barnard et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Engineering of biotin-prototrophy in Pichia pastoris for robust production processes

(2010) Brigitte Gasser et al.   METABOLIC ENGINEERING

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

(2010) Bevan KS Chung et al.   Microbial Cell Factories

High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors

(2010) Takahiro Sasagawa et al.   PLASMID

Maltose-Binding Protein Enhances Secretion of Recombinant Human Granzyme B Accompanied by In Vivo Processing of a Precursor MBP Fusion Protein

(2010) Benjamin Dälken et al.   PLoS One

A high-throughput purification of monoclonal antibodies from glycoengineered Pichia pastoris

(2010) Youwei Jiang et al.   PROTEIN EXPRESSION AND PURIFICATION

High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins

(2010) Kimihiko Mizutani et al.   PROTEIN EXPRESSION AND PURIFICATION

Oxidative Protein Folding in the Secretory Pathway and Redox Signaling Across Compartments and Cells

(2010) Éva Margittai et al.   TRAFFIC

Isolation of Pichia pastoris PIR genes and their utilization for cell surface display and recombinant protein secretion

(2010) Yogender Pal Khasa et al.   YEAST

Efficient vectors for expression cloning of large numbers of PCR fragments inP. pastoris

(2010) Ben Rao et al.   YEAST

A combinatorial genetic library approach to target heterologous glycosylation enzymes to the endoplasmic reticulum or the Golgi apparatus of Pichia pastoris

(2010) Juergen H. Nett et al.   YEAST

ER and aging—Protein folding and the ER stress response

(2009) Nirinjini Naidoo   AGEING RESEARCH REVIEWS

Membrane Traffic Within the Golgi Apparatus

(2009) Benjamin S. Glick et al.   Annual Review of Cell and Developmental Biology

Phosphate-Responsive Promoter of a Pichia pastoris Sodium Phosphate Symporter

(2009) J. Ahn et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production inPichia pastoris

(2009) Gerhard Stadlmayr et al.   BIOTECHNOLOGY AND BIOENGINEERING

A new screening method based on yeast-expressed human dipeptidyl peptidase IV and discovery of novel inhibitors

(2009) Chen-Xi Hu et al.   BIOTECHNOLOGY LETTERS

The yeast Golgi apparatus: Insights and mysteries

(2009) Effrosyni Papanikou et al.   FEBS LETTERS

Yeast systems biotechnology for the production of heterologous proteins

(2009) Alexandra Graf et al.   FEMS YEAST RESEARCH

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Mechanism and components of endoplasmic reticulum-associated degradation

(2009) J. Hoseki et al.   JOURNAL OF BIOCHEMISTRY

Stress-sensing mechanisms in the unfolded protein response: similarities and differences between yeast and mammals

(2009) K. Kohno   JOURNAL OF BIOCHEMISTRY

The Effect of Temperature on the Proteome of RecombinantPichia pastoris

(2009) Martin Dragosits et al.   JOURNAL OF PROTEOME RESEARCH

Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

(2009) Marc Carnicer et al.   Microbial Cell Factories

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

(2009) Diethard Mattanovich et al.   Microbial Cell Factories

Genome sequence of the recombinant protein production host Pichia pastoris

(2009) Kristof De Schutter et al.   NATURE BIOTECHNOLOGY

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

(2009) David Resina et al.   New Biotechnology

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

(2008) Alexandra Graf et al.   BMC GENOMICS

Pichia Power: India's Biotech Industry Puts Unconventional Yeast to Work

(2008) Chandra Shekhar   CHEMISTRY & BIOLOGY

Protein quality control in the early secretory pathway

(2008) Tiziana Anelli et al.   EMBO JOURNAL

Posttransformational vector amplification in the yeastPichia pastoris

(2008) Anthony Jay Sunga et al.   FEMS YEAST RESEARCH

One step at a time: endoplasmic reticulum-associated degradation

(2008) Shruthi S. Vembar et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH

Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals

(2008) Anand Ghosalkar et al.   PROTEIN EXPRESSION AND PURIFICATION

Hypoxic fed-batch cultivation ofPichia pastoris increases specific and volumetric productivity of recombinant proteins

(2007) Kristin Baumann et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-level expression, purification and characterization of recombinant Aspergillus oryzae alkaline protease in Pichia pastoris

(2007) Ji-Ping Guo et al.   PROTEIN EXPRESSION AND PURIFICATION