Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids

The pattern of nodal morphogen signaling is shaped by co-receptor expression

(2021) Nathan D Lord et al.   eLife

Integration of Nodal and BMP Signaling by Mutual Signaling Effector Antagonism

(2020) Gary Huiming Soh et al.   Cell Reports

An in vitro model of early anteroposterior organization during human development

(2020) Naomi Moris et al.   NATURE

High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

(2020) Kyaw Thu Minn et al.   eLife

Synergy with TGFβ ligands switches WNT pathway dynamics from transient to sustained during human pluripotent cell differentiation

(2019) Joseph Massey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid changes in morphogen concentration control self-organized patterning in human embryonic stem cells

(2019) Idse Heemskerk et al.   eLife

Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids

(2019) Sapna Chhabra et al.   PLOS BIOLOGY

Nodal and BMP dispersal during early zebrafish development

(2018) Katherine W. Rogers et al.   DEVELOPMENTAL BIOLOGY

Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages

(2018) Antonius L. van Boxtel et al.   DEVELOPMENTAL CELL

Morphogen gradient reconstitution reveals Hedgehog pathway design principles

(2018) Pulin Li et al.   SCIENCE

Scale-invariant patterning by size-dependent inhibition of Nodal signalling

(2018) María Almuedo-Castillo et al.   NATURE CELL BIOLOGY

Gdf3 is required for robust Nodal signaling during germ layer formation and left-right patterning

(2017) Jose L Pelliccia et al.   eLife

Vg1-Nodal heterodimers are the endogenous inducers of mesendoderm

(2017) Tessa G Montague et al.   eLife

Maternal Gdf3 is an obligatory cofactor in Nodal signaling for embryonic axis formation in zebrafish

(2017) Brent W Bisgrove et al.   eLife

A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila

(2017) Stefan Harmansa et al.   eLife

Nodal patterning without Lefty inhibitory feedback is functional but fragile

(2017) Katherine W Rogers et al.   eLife

A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization

(2016) Fred Etoc et al.   DEVELOPMENTAL CELL

Visualization of a short-range Wnt gradient in the intestinal stem-cell niche

(2016) Henner F. Farin et al.   NATURE

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Self-organization of human embryonic stem cells on micropatterns

(2016) Alessia Deglincerti et al.   Nature Protocols

Extracellular interactions and ligand degradation shape the nodal morphogen gradient

(2016) Yin Wang et al.   eLife

A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain

(2015) Antonius L. van Boxtel et al.   DEVELOPMENTAL CELL

Nodal·Gdf1 Heterodimers with Bound Prodomains Enable Serum-independent Nodal Signaling and Endoderm Differentiation

(2014) Christophe Fuerer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells

(2014) Aryeh Warmflash et al.   NATURE METHODS

Patterning and growth control by membrane-tethered Wingless

(2013) Cyrille Alexandre et al.   NATURE

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Differential Diffusivity of Nodal and Lefty Underlies a Reaction-Diffusion Patterning System

(2012) P. Muller et al.   SCIENCE

An Efficient and Reversible Transposable System for Gene Delivery and Lineage-Specific Differentiation in Human Embryonic Stem Cells

(2009) Arnaud Lacoste et al.   Cell Stem Cell

TGF-β Superfamily Signaling in Embryonic Development and Homeostasis

(2009) Mary Y. Wu et al.   DEVELOPMENTAL CELL

Nodal Morphogens

(2009) A. F. Schier   Cold Spring Harbor Perspectives in Biology

PCR-amplification of GC-rich regions: 'slowdown PCR'

(2008) Ulrich H Frey et al.   Nature Protocols