Replication and single-cycle delivery of SARS-CoV-2 replicons

Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation

(2021) Christopher P. Lapointe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering a Reliable and Convenient SARS-CoV-2 Replicon System for Analysis of Viral RNA Synthesis and Screening of Antiviral Inhibitors

(2021) Yuewen Luo et al.   mBio

A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence

(2021) Xianwen Zhang et al.   CELL

Emergence of a SARS-CoV-2 variant of concern with mutations in spike glycoprotein

(2021) Houriiyah Tegally et al.   NATURE

A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research

(2021) Suzannah J. Rihn et al.   PLOS BIOLOGY

A novel cell culture system modeling the SARS-CoV-2 life cycle

(2021) Xiaohui Ju et al.   PLoS Pathogens

Overcoming culture restriction for SARS-CoV-2 in human cells facilitates the screening of compounds inhibiting viral replication

(2021) Santseharay Ramirez et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis

(2021) Yaara Finkel et al.   NATURE

Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing

(2021) Xi He et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes

(2021) Irwin Jungreis et al.   Nature Communications

Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction

(2021) Shiho Torii et al.   Cell Reports

Masitinib is a broad coronavirus 3CL inhibitor that blocks replication of SARS-CoV-2

(2021) Nir Drayman et al.   SCIENCE

Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China

(2020) Aiping Wu et al.   Cell Host & Microbe

Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

(2020) Daniel K W Chu et al.   CLINICAL CHEMISTRY

Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses

(2020) Michael Letko et al.   Nature Microbiology

SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract

(2020) Yixuan J. Hou et al.   CELL

Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19

(2020) Daniel Blanco-Melo et al.   CELL

Viral replicons as valuable tools for drug discovery

(2020) Holger Hannemann   DRUG DISCOVERY TODAY

Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency

(2020) Calvin J. Gordon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

(2020) Tran Thi Nhu Thao et al.   NATURE

Structure of the RNA-dependent RNA polymerase from COVID-19 virus

(2020) Yan Gao et al.   SCIENCE

Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays

(2020) Katharine H. D. Crawford et al.   Viruses-Basel

Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

(2020) Xiuyuan Ou et al.   Nature Communications

Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses

(2020) Fabian Schmidt et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Identification of common deletions in the spike protein of SARS-CoV-2

(2020) Zhe Liu et al.   JOURNAL OF VIROLOGY

Convergent antibody responses to SARS-CoV-2 in convalescent individuals

(2020) Davide F. Robbiani et al.   NATURE

Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2

(2020) Matthias Thoms et al.   SCIENCE

Activation and evasion of type I interferon responses by SARS-CoV-2

(2020) Xiaobo Lei et al.   Nature Communications

Remdesivir Inhibits SARS-CoV-2 in Human Lung Cells and Chimeric SARS-CoV Expressing the SARS-CoV-2 RNA Polymerase in Mice

(2020) Andrea J. Pruijssers et al.   Cell Reports

THE CHOLESTEROL METABOLITE 27-HYDROXYCHOLESTEROL INHIBITS SARS-CoV-2 AND IS MARKEDLY DECREASED IN COVID-19 PATIENTS

(2020) Alessandro Marcello et al.   Redox Biology

SARS-CoV-2 Disrupts Splicing, Translation, and Protein Trafficking to Suppress Host Defenses

(2020) Abhik K. Banerjee et al.   CELL

SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation

(2020) Katharina Schubert et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evasion of Type I Interferon by SARS-CoV-2

(2020) Hongjie Xia et al.   Cell Reports

A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2

(2020) Yang Zhang et al.   ANTIVIRAL RESEARCH

Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

(2020) William M. Schneider et al.   CELL

TMEM41B Is a Pan-flavivirus Host Factor

(2020) H.-Heinrich Hoffmann et al.   CELL

Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles

(2020) Maolin Lu et al.   Cell Host & Microbe

The viral protein NSP1 acts as a ribosome gatekeeper for shutting down host translation and fostering SARS-CoV-2 translation

(2020) Antonin TIDU et al.   RNA

SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target

(2019) Shuofeng Yuan et al.   Nature Communications

Hepatitis C virus cell culture models: an encomium on basic research paving the road to therapy development

(2018) Volker Lohmann   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

(2016) Natalay Kouprina et al.   CHROMOSOMA

Continuous and Discontinuous RNA Synthesis in Coronaviruses

(2015) Isabel Sola et al.   Annual Review of Virology

Severe Acute Respiratory Syndrome Coronavirus Protein nsp1 Is a Novel Eukaryotic Translation Inhibitor That Represses Multiple Steps of Translation Initiation

(2012) K. G. Lokugamage et al.   JOURNAL OF VIROLOGY

Structure and Functional Relevance of a Transcription-Regulating Sequence Involved in Coronavirus Discontinuous RNA Synthesis

(2011) D. Dufour et al.   JOURNAL OF VIROLOGY

A two-pronged strategy to suppress host protein synthesis by SARS coronavirus Nsp1 protein

(2009) Wataru Kamitani et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

T7 RNA polymerase-dependent and -independent systems for cDNA-based rescue of Rift Valley fever virus

(2008) M. Habjan et al.   JOURNAL OF GENERAL VIROLOGY

The M, E, and N Structural Proteins of the Severe Acute Respiratory Syndrome Coronavirus Are Required for Efficient Assembly, Trafficking, and Release of Virus-Like Particles

(2008) Y. L. Siu et al.   JOURNAL OF VIROLOGY

Severe Acute Respiratory Syndrome Coronavirus nsp1 Suppresses Host Gene Expression, Including That of Type I Interferon, in Infected Cells

(2008) K. Narayanan et al.   JOURNAL OF VIROLOGY