Transformation-associated recombination (TAR) cloning for genomics studies and synthetic biology

Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining

(2015) Xiaoyu Tang et al.   ACS Chemical Biology

High-throughput plasmid construction using homologous recombination in yeast: its mechanisms and application to protein production for X-ray crystallography

(2015) Kimihiko Mizutani   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations

(2015) Min Wang et al.   BMC GENOMICS

Stable maintenance ofde novoassembled human artificial chromosomes in embryonic stem cells and their differentiated progeny in mice

(2015) Mikhail Liskovykh et al.   CELL CYCLE

Genome Mining ofStreptomycessp. Tü 6176: Characterization of the Nataxazole Biosynthesis Pathway

(2015) Carolina Cano-Prieto et al.   CHEMBIOCHEM

Critical Intermediates Reveal New Biosynthetic Events in the Enigmatic Colibactin Pathway

(2015) Zhong-Rui Li et al.   CHEMBIOCHEM

A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges

(2015) Mitsuo Oshimura et al.   CHROMOSOME RESEARCH

Genetic and epigenetic regulation of centromeres: a look at HAC formation

(2015) Jun-ichirou Ohzeki et al.   CHROMOSOME RESEARCH

Strategies for cloning and manipulating natural and synthetic chromosomes

(2015) Bogumil J. Karas et al.   CHROMOSOME RESEARCH

De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications

(2015) Robert L. Katona   CHROMOSOME RESEARCH

Developing de novo human artificial chromosomes in embryonic stem cells using HSV-1 amplicon technology

(2015) Daniela Moralli et al.   CHROMOSOME RESEARCH

Versatile genetic assembly system (VEGAS) to assemble pathways for expression inS. cerevisiae

(2015) Leslie A. Mitchell et al.   NUCLEIC ACIDS RESEARCH

Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

(2015) Yongxin Li et al.   Scientific Reports

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways

(2015) Yongbo Yuan et al.   ACS Synthetic Biology

Engineering Modular Viral Scaffolds for Targeted Bacterial Population Editing

(2015) Hiroki Ando et al.   Cell Systems

Viral Hybrid-Vectors for Delivery of Autonomous Replicons

(2014) Wenli Zhang et al.   CURRENT GENE THERAPY

Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology

(2014) Natalay Kouprina et al.   Expert Opinion on Drug Delivery

Direct Capture and Heterologous Expression of Salinispora Natural Product Genes for the Biosynthesis of Enterocin

(2014) Bailey Bonet et al.   JOURNAL OF NATURAL PRODUCTS

Whole-genome haplotyping using long reads and statistical methods

(2014) Volodymyr Kuleshov et al.   NATURE BIOTECHNOLOGY

Biosynthesis of polybrominated aromatic organic compounds by marine bacteria

(2014) Vinayak Agarwal et al.   Nature Chemical Biology

Programming biological operating systems: genome design, assembly and activation

(2014) Daniel G Gibson   NATURE METHODS

A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function

(2014) Artem V. Kononenko et al.   NUCLEIC ACIDS RESEARCH

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Targeted Capture and Heterologous Expression of the Pseudoalteromonas Alterochromide Gene Cluster in Escherichia coli Represents a Promising Natural Product Exploratory Platform

(2014) Avena C. Ross et al.   ACS Synthetic Biology

Recent advances in the heterologous expression of microbial natural product biosynthetic pathways

(2013) Sarah E. Ongley et al.   NATURAL PRODUCT REPORTS

Assembly of eukaryotic algal chromosomes in yeast

(2013) Bogumil J Karas et al.   Journal of Biological Engineering

Overexpression ofO-methyltransferase leads to improved vanillin production in baker's yeast only when complemented with model-guided network engineering

(2012) Ana Rita Brochado et al.   BIOTECHNOLOGY AND BIOENGINEERING

A new generation of human artificial chromosomes for functional genomics and gene therapy

(2012) Natalay Kouprina et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

HACking the centromere chromatin code: insights from human artificial chromosomes

(2012) Jan H. Bergmann et al.   CHROMOSOME RESEARCH

Exclusion of the 750-kb genetically unstable region at Xq27 as a candidate locus for prostate malignancy in HPCX1-linked families

(2012) Natalay Kouprina et al.   GENES CHROMOSOMES & CANCER

Sequence analysis of a complete 1.66 Mb Prochlorococcus marinus MED4 genome cloned in yeast

(2012) Christian Tagwerker et al.   NUCLEIC ACIDS RESEARCH

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

(2012) P. J. Westfall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly of Large, High G+C Bacterial DNA Fragments in Yeast

(2012) Vladimir N. Noskov et al.   ACS Synthetic Biology

Human Artificial Chromosomes for Gene Delivery and the Development of Animal Models

(2011) Yasuhiro Kazuki et al.   MOLECULAR THERAPY

Therapeutic in vivo gene transfer for genetic disease using AAV: progress and challenges

(2011) Federico Mingozzi et al.   NATURE REVIEWS GENETICS

Cloning of the Repertoire of Individual Plasmodium falciparum var Genes Using Transformation Associated Recombination (TAR)

(2011) Annette Gaida et al.   PLoS One

Human artificial chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells

(2011) J.-H. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional analysis of environmental DNA-derived type II polyketide synthases reveals structurally diverse secondary metabolites

(2011) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stem Cell-Mediated Transfer of a Human Artificial Chromosome Ameliorates Muscular Dystrophy

(2011) F. S. Tedesco et al.   Science Translational Medicine

Cloning the Acholeplasma laidlawii PG-8A Genome in Saccharomyces cerevisiae as a Yeast Centromeric Plasmid

(2011) Bogumil J. Karas et al.   ACS Synthetic Biology

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

(2010) Jeffrey H. Kim et al.   BIOPOLYMERS

Retroviral vectors for gene therapy

(2010) Patrick Maier et al.   Future Microbiology

Cloning whole bacterial genomes in yeast

(2010) Gwynedd A. Benders et al.   NUCLEIC ACIDS RESEARCH

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

(2010) D. G. Gibson et al.   SCIENCE

Progress and prospects: Biological properties and technological advances of herpes simplex virus type 1-based amplicon vectors

(2009) A L Epstein   GENE THERAPY

Integration-deficient Lentiviral Vectors: A Slow Coming of Age

(2009) Klaus Wanisch et al.   MOLECULAR THERAPY

Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast

(2009) C. Lartigue et al.   SCIENCE

Lentiviral vectors with measles virus glycoproteins – dream team for gene transfer?

(2009) Christian J. Buchholz et al.   TRENDS IN BIOTECHNOLOGY

Isolation and analysis of the genetic diversity of repertoires of VSG expression site containing telomeres from Trypanosoma brucei gambiense, T. b. brucei and T. equiperdum

(2008) Rosanna Young et al.   BMC GENOMICS

Inactivation of a Human Kinetochore by Specific Targeting of Chromatin Modifiers

(2008) Megumi Nakano et al.   DEVELOPMENTAL CELL

Advances in High-capacity Extrachromosomal Vector Technology: Episomal Maintenance, Vector Delivery, and Transgene Expression

(2008) Michele MP Lufino et al.   MOLECULAR THERAPY

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

(2008) Natalay Kouprina et al.   Nature Protocols

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH

One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome

(2008) D. G. Gibson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome

(2008) D. G. Gibson et al.   SCIENCE