Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation

Phase separation directs ubiquitination of gene-body nucleosomes

(2020) Laura D. Gallego et al.   NATURE

Composition-dependent thermodynamics of intracellular phase separation

(2020) Joshua A. Riback et al.   NATURE

A quantitative inventory of yeast P body proteins reveals principles of composition and specificity

(2020) Wenmin Xing et al.   eLife

Enzymatic Reactions inside Biological Condensates

(2020) Yi Zhang et al.   JOURNAL OF MOLECULAR BIOLOGY

Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality

(2019) Sethuramasundaram Pitchiaya et al.   MOLECULAR CELL

DEAD-box ATPases are global regulators of phase-separated organelles

(2019) Maria Hondele et al.   NATURE

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

Pat1 activates late steps in mRNA decay by multiple mechanisms

(2019) Joseph H. Lobel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Atomic-level insight into mRNA processing bodies by combining solid and solution-state NMR spectroscopy

(2019) Reinier Damman et al.   Nature Communications

P-Bodies: Composition, Properties, and Functions

(2018) Yang Luo et al.   BIOCHEMISTRY

Phase Transitions in the Assembly and Function of Human miRISC

(2018) Jessica Sheu-Gruttadauria et al.   CELL

Control of mRNA decapping by autoinhibition

(2018) David R Paquette et al.   NUCLEIC ACIDS RESEARCH

mRNA structure determines specificity of a polyQ-driven phase separation

(2018) Erin M. Langdon et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis

(2018) Jeffrey S. Mugridge et al.   Nature Communications

Context-dependent deposition and regulation of mRNAs in P-bodies

(2018) Congwei Wang et al.   eLife

Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability

(2018) Leon Y Chan et al.   eLife

Structural and molecular mechanisms for the control of eukaryotic 5′–3′ mRNA decay

(2018) Jeffrey S. Mugridge et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

General decapping activators target different subsets of inefficiently translated mRNAs

(2018) Feng He et al.   eLife

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

(2017) Arnaud Hubstenberger et al.   MOLECULAR CELL

The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells

(2017) Ivana Horvathova et al.   MOLECULAR CELL

An improved MS2 system for accurate reporting of the mRNA life cycle

(2017) Evelina Tutucci et al.   NATURE METHODS

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping

(2017) Stefan Schütz et al.   NUCLEIC ACIDS RESEARCH

Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme

(2017) Jan Philip Wurm et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Phase separation in biology; functional organization of a higher order

(2016) Diana M. Mitrea et al.   Cell Communication and Signaling

TheS. pombemRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface

(2016) Jan Philip Wurm et al.   RNA

ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

(2016) Christopher Frederick Mugler et al.   eLife

Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain

(2015) Feng He et al.   RNA

In Vitro Reconstitution of a Cellular Phase-Transition Process that Involves the mRNA Decapping Machinery

(2014) Simon A. Fromm et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

NMRFAM-SPARKY: enhanced software for biomolecular NMR spectroscopy

(2014) Woonghee Lee et al.   BIOINFORMATICS

Quantifying mRNA targeting to P-bodies in living human cells reveals their dual role in mRNA decay and storage

(2014) Adva Aizer et al.   JOURNAL OF CELL SCIENCE

The role of disordered protein regions in the assembly of decapping complexes and RNP granules

(2013) S. Jonas et al.   GENES & DEVELOPMENT

Synthesis of nucleoside phosphosulfates

(2012) Joanna Kowalska et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Interdomain dynamics and coactivation of the mRNA decapping enzyme Dcp2 are mediated by a gatekeeper tryptophan

(2012) S. N. Floor et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex

(2011) Simon A Fromm et al.   EMBO JOURNAL

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy

(2010) Mary Anne Refaei et al.   JOURNAL OF BIOMOLECULAR NMR

Identification and Analysis of the Interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae

(2010) Y. Harigaya et al.   MOLECULAR AND CELLULAR BIOLOGY

mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2

(2008) Mandar V. Deshmukh et al.   MOLECULAR CELL

Structural Basis of Dcp2 Recognition and Activation by Dcp1

(2008) Meipei She et al.   MOLECULAR CELL