Genetic Code Expansion Through Quadruplet Codon Decoding

Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons

(2021) Jeffery M. Tharp et al.   ACS Chemical Biology

A 68-codon genetic code to incorporate four distinct non-canonical amino acids enabled by automated orthogonal mRNA design

(2021) Daniel L. Dunkelmann et al.   Nature Chemistry

Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids

(2020) Daniel L. Dunkelmann et al.   Nature Chemistry

Mutually orthogonal nonsense-suppression systems and conjugation chemistries for precise protein labeling at up to three distinct sites

(2019) James S Italia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetic Incorporation of Noncanonical Amino Acids Using Two Mutually Orthogonal Quadruplet Codons

(2019) Erome Daniel Hankore et al.   ACS Synthetic Biology

A General Strategy for Engineering Noncanonical Amino Acid Dependent Bacterial Growth

(2019) Minseob Koh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Playing with the Molecules of Life

(2018) Douglas D. Young et al.   ACS Chemical Biology

Functional Replacement of Histidine in Proteins To Generate Noncanonical Amino Acid Dependent Organisms

(2018) Fei Gan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs

(2018) Julian C. W. Willis et al.   Nature Chemistry

Controlling the Replication of a Genomically Recoded HIV-1 with a Functional Quadruplet Codon in Mammalian Cells

(2018) Yan Chen et al.   ACS Synthetic Biology

Expanding and reprogramming the genetic code

(2017) Jason W. Chin   NATURE

Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells

(2017) Robert Serfling et al.   NUCLEIC ACIDS RESEARCH

Controlling Multicycle Replication of Live-Attenuated HIV-1 Using an Unnatural Genetic Switch

(2017) Zhe Yuan et al.   ACS Synthetic Biology

Design, synthesis, and testing toward a 57-codon genome

(2016) N. Ostrov et al.   SCIENCE

Generation of influenza A viruses as live but replication-incompetent virus vaccines

(2016) L. Si et al.   SCIENCE

Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria

(2016) Nanxi Wang et al.   Scientific Reports

Recoded organisms engineered to depend on synthetic amino acids

(2015) Alexis J. Rovner et al.   NATURE

Biocontainment of genetically modified organisms by synthetic protein design

(2015) Daniel J. Mandell et al.   NATURE

Designing logical codon reassignment – Expanding the chemistry in biology

(2015) Anaëlle Dumas et al.   Chemical Science

Construction of a Live-Attenuated HIV-1 Vaccine through Genetic Code Expansion

(2014) Nanxi Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Bacterial Strain with a Unique Quadruplet Codon Specifying Non-native Amino Acids

(2014) Abhishek Chatterjee et al.   CHEMBIOCHEM

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET

(2014) Kaihang Wang et al.   Nature Chemistry

An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon

(2013) Wei Niu et al.   ACS Chemical Biology

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

(2012) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome

(2010) Heinz Neumann et al.   NATURE

Synthesis at the Interface of Chemistry and Biology

(2009) Xu Wu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Gripping Tale of Ribosomal Frameshifting: Extragenic Suppressors of Frameshift Mutations Spotlight P-Site Realignment

(2009) J. F. Atkins et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Evidence from glycine transfer RNA of a frozen accident at the dawn of the genetic code

(2008) Harold S Bernhardt et al.   Biology Direct

Four-base codon-mediated saturation mutagenesis in a cell-free translation system

(2008) Takayoshi Watanabe et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

(2008) Tatsuo Yanagisawa et al.   JOURNAL OF MOLECULAR BIOLOGY

A structural understanding of the dynamic ribosome machine

(2008) Thomas A. Steitz   NATURE REVIEWS MOLECULAR CELL BIOLOGY