Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells

Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1

(2017) Yue Guan et al.   Environmental Microbiology Reports

Debugging Eukaryotic Genetic Code Expansion for Site-Specific Click-PAINT Super-Resolution Microscopy

(2016) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics

(2016) He Tian et al.   CHEMICAL REVIEWS

On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme

(2015) Claus-D. Kuhn et al.   CELL

Labeling proteins on live mammalian cells using click chemistry

(2015) Ivana Nikić et al.   Nature Protocols

Rationally evolving tRNAPylfor efficient incorporation of noncanonical amino acids

(2015) Chenguang Fan et al.   NUCLEIC ACIDS RESEARCH

Minimal Tags for Rapid Dual-Color Live-Cell Labeling and Super-Resolution Microscopy

(2014) Ivana Nikić et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool

(2014) Wei Wan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins

(2014) Kathrin Lang et al.   CHEMICAL REVIEWS

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

(2014) Wolfgang H. Schmied et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon

(2013) Wei Niu et al.   ACS Chemical Biology

Genetic Incorporation of Multiple Unnatural Amino Acids into Proteins in Mammalian Cells

(2013) Han Xiao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Versatile Platform for Single- and Multiple-Unnatural Amino Acid Mutagenesis in Escherichia coli

(2013) Abhishek Chatterjee et al.   BIOCHEMISTRY

Genetically Encoded Chemical Probes in Cells Reveal the Binding Path of Urocortin-I to CRF Class B GPCR

(2013) Irene Coin et al.   CELL

Transfer RNA Post-Transcriptional Processing, Turnover, and Subcellular Dynamics in the YeastSaccharomyces cerevisiae

(2013) Anita K. Hopper   GENETICS

Nonsense and Sense Suppression Abilities of Original and Derivative Methanosarcina mazei Pyrrolysyl-tRNA Synthetase-tRNAPyl Pairs in the Escherichia coli BL21(DE3) Cell Strain

(2013) Keturah A. Odoi et al.   PLoS One

Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

(2013) A. Chatterjee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Amino Acids for Diels-Alder Reactions in Living Cells

(2012) Tilman Plass et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPylfor genetic code expansion

(2012) Patrick O'Donoghue et al.   FEBS LETTERS

Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions

(2012) Kathrin Lang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction

(2012) Kathrin Lang et al.   Nature Chemistry

Photo-Cross-Linkers Incorporated into G-Protein-Coupled Receptors in Mammalian Cells: A Ligand Comparison

(2011) Irene Coin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Human Mitochondrial tRNAs: Biogenesis, Function, Structural Aspects, and Diseases

(2011) Tsutomu Suzuki et al.   Annual Review of Genetics

Mechanism of protein biosynthesis in mammalian mitochondria

(2011) Brooke E. Christian et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

A Facile System for Genetic Incorporation of Two Different Noncanonical Amino Acids into One Protein inEscherichia coli

(2010) Wei Wan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization

(2010) Heike Betat et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Proteins Containing Nonnatural Amino Acids

(2009) Jiantao Guo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Do all modifications benefit all tRNAs?

(2009) Eric M. Phizicky et al.   FEBS LETTERS

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

(2009) Jeffrey K. Takimoto et al.   Molecular BioSystems

Pyrrolysyl-tRNA synthetase–tRNAPyl structure reveals the molecular basis of orthogonality

(2009) Kayo Nozawa et al.   NATURE

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

(2008) Takahito Mukai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification

(2008) Tatsuo Yanagisawa et al.   CHEMISTRY & BIOLOGY

The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases

(2008) Caroline Köhrer et al.   METHODS

tRNAdb 2009: compilation of tRNA sequences and tRNA genes

(2008) F. Juhling et al.   NUCLEIC ACIDS RESEARCH