Preclinical tumor organoid models in personalized cancer therapy: Not everyone fits the mold

Evolution of organoid technology: Lessons learnt in Co-Culture systems from developmental biology

(2021) Ensieh Zahmatkesh et al.   DEVELOPMENTAL BIOLOGY

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

(2020) Hongwei Shao et al.   Jove-Journal of Visualized Experiments

Challenges in Establishing Pure Lung Cancer Organoids Limit Their Utility for Personalized Medicine

(2020) Krijn K. Dijkstra et al.   Cell Reports

Organoid Models of Tumor Immunology

(2020) Kanako Yuki et al.   TRENDS IN IMMUNOLOGY

Patient-Derived Ovarian Cancer Organoids Mimic Clinical Response and Exhibit Heterogeneous Inter- and Intrapatient Drug Responses

(2020) Chris Jenske de Witte et al.   Cell Reports

Cancer-Associated Fibroblasts Provide a Stromal Niche for Liver Cancer Organoids That Confers Trophic Effects and Therapy Resistance

(2020) Jiaye Liu et al.   Cellular and Molecular Gastroenterology and Hepatology

Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

(2019) Tristan Courau et al.   Journal for ImmunoTherapy of Cancer

3D model for CAR‐mediated cytotoxicity using patient‐derived colorectal cancer organoids

(2019) Theresa E Schnalzger et al.   EMBO JOURNAL

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity

(2019) Oded Kopper et al.   NATURE MEDICINE

Oral mucosal organoids as a potential platform for personalized cancer therapy

(2019) Else Driehuis et al.   Cancer Discovery

Patient-derived organoids from endometrial disease capture clinical heterogeneity and are amenable to drug screening

(2019) Matteo Boretto et al.   NATURE CELL BIOLOGY

A rectal cancer organoid platform to study individual responses to chemoradiation

(2019) Karuna Ganesh et al.   NATURE MEDICINE

Patient-derived organoids can predict response to chemotherapy in metastatic colorectal cancer patients

(2019) Salo N. Ooft et al.   Science Translational Medicine

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity

(2019) Fadi Jacob et al.   CELL

Tumor organoid–T-cell coculture systems

(2019) Chiara M. Cattaneo et al.   Nature Protocols

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

(2018) Takashi Seino et al.   Cell Stem Cell

Development of a new patient-derived xenograft humanised mouse model to study human-specific tumour microenvironment and immunotherapy

(2018) Yue Zhao et al.   GUT

Emerging tumor spheroids technologies for 3D in vitro cancer modeling

(2018) Tânia Rodrigues et al.   PHARMACOLOGY & THERAPEUTICS

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Personalized Cancer Medicine: An Organoid Approach

(2018) Hamidreza Aboulkheyr Es et al.   TRENDS IN BIOTECHNOLOGY

Organoid profiling identifies common responders to chemotherapy in pancreatic cancer

(2018) Hervé Tiriac et al.   Cancer Discovery

Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity

(2018) Gianpiero Lazzari et al.   Acta Biomaterialia

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

(2018) Krijn K. Dijkstra et al.   CELL

3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade

(2018) Amir R. Aref et al.   LAB ON A CHIP

A Comprehensive Human Gastric Cancer Organoid Biobank Captures Tumor Subtype Heterogeneity and Enables Therapeutic Screening

(2018) Helen H.N. Yan et al.   Cell Stem Cell

Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition

(2018) Masayuki Fujii et al.   Cell Stem Cell

Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer

(2018) Shunsuke Kitajima et al.   Cancer Discovery

In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis

(2017) Jatin Roper et al.   NATURE BIOTECHNOLOGY

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

(2017) Laura Broutier et al.   NATURE MEDICINE

Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer

(2017) Jarno Drost et al.   SCIENCE

Clinical responses to adoptive T-cell transfer can be modeled in an autologous immune-humanized mouse model

(2017) Henrik Jespersen et al.   Nature Communications

Personalized In Vitro and In Vivo Cancer Models to Guide Precision Medicine

(2017) Chantal Pauli et al.   Cancer Discovery

Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids

(2017) Russell W. Jenkins et al.   Cancer Discovery

A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo

(2016) Christopher G. Hubert et al.   CANCER RESEARCH

Engineering Stem Cell Organoids

(2016) Xiaolei Yin et al.   Cell Stem Cell

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Organoid culture systems for prostate epithelial and cancer tissue

(2016) Jarno Drost et al.   Nature Protocols

Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research

(2016) Paul W. Tetteh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis

(2016) Johanna F. Dekkers et al.   Science Translational Medicine

DNA-Guided Precision Medicine for Cancer: A Case of Irrational Exuberance?

(2016) E. E. Voest et al.   Cancer Discovery

Drug response in organoids generated from frozen primary tumor tissues

(2016) Alex J. Walsh et al.   Scientific Reports

Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

(2016) Carla S Verissimo et al.   eLife

Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices

(2015) Skarphedinn Halldorsson et al.   BIOSENSORS & BIOELECTRONICS

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Organoid Models of Human and Mouse Ductal Pancreatic Cancer

(2015) Sylvia F. Boj et al.   CELL

Organoid development in cancer genome discovery

(2015) Dong Gao et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection

(2015) Sina Bartfeld et al.   GASTROENTEROLOGY

Tumor-Derived Cell Lines as Molecular Models of Cancer Pharmacogenomics

(2015) A. Goodspeed et al.   MOLECULAR CANCER RESEARCH

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Three Dimensional Culture of Human Renal Cell Carcinoma Organoids

(2015) Cynthia A. Batchelder et al.   PLoS One

Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases

(2015) Fleur Weeber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microenvironmental regulation of therapeutic response in cancer

(2015) Florian Klemm et al.   TRENDS IN CELL BIOLOGY

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

(2015) Benjamin S. Freedman et al.   Nature Communications

A bladder cancer microenvironment simulation system based on a microfluidic co-culture model

(2015) Peng-fei Liu et al.   Oncotarget

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

Critical analysis of 3-D organoid in vitro cell culture models for high-throughput drug candidate toxicity assessments

(2014) Anna Astashkina et al.   ADVANCED DRUG DELIVERY REVIEWS

“In vitro” 3D models of tumor-immune system interaction

(2014) Christian Hirt et al.   ADVANCED DRUG DELIVERY REVIEWS

Properties of AdeABC and AdeIJK Efflux Systems of Acinetobacter baumannii Compared with Those of the AcrAB-TolC System of Escherichia coli

(2014) Etsuko Sugawara et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Cancer Cell Lines for Drug Discovery and Development

(2014) J. L. Wilding et al.   CANCER RESEARCH

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

Identification of Multipotent Luminal Progenitor Cells in Human Prostate Organoid Cultures

(2014) Wouter R. Karthaus et al.   CELL

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Whole-genome sequencing and comprehensive molecular profiling identify new driver mutations in gastric cancer

(2014) Kai Wang et al.   NATURE GENETICS

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture

(2014) Xingnan Li et al.   NATURE MEDICINE

Personalized In Vitro Cancer Modeling — Fantasy or Reality?

(2014) Richard Bartlett et al.   Translational Oncology

Patient-Derived Xenograft Models: An Emerging Platform for Translational Cancer Research

(2014) M. Hidalgo et al.   Cancer Discovery

Establishment of a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells within the stem cell niche

(2013) Takahito Katano et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A Renewable Tissue Resource of Phenotypically Stable, Biologically and Ethnically Diverse, Patient-Derived Human Breast Cancer Xenograft Models

(2013) X. Zhang et al.   CANCER RESEARCH

Collective Invasion in Breast Cancer Requires a Conserved Basal Epithelial Program

(2013) Kevin J. Cheung et al.   CELL

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

A functional CFTR assay using primary cystic fibrosis intestinal organoids

(2013) Johanna F Dekkers et al.   NATURE MEDICINE

The role of tumour–stromal interactions in modifying drug response: challenges and opportunities

(2013) Douglas W. McMillin et al.   NATURE REVIEWS DRUG DISCOVERY

Effect of homotypic and heterotypic interaction in 3D on the E-selectin mediated adhesive properties of breast cancer cell lines

(2012) Siddarth Chandrasekaran et al.   BIOMATERIALS

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Cross talk between cancer and immune cells: exploring complex dynamics in a microfluidic environment

(2012) Luca Businaro et al.   LAB ON A CHIP

Patient-derived tumour xenografts as models for oncology drug development

(2012) John J. Tentler et al.   Nature Reviews Clinical Oncology

Mouse Models of Cancer

(2011) Dong-Joo Cheon et al.   Annual Review of Pathology-Mechanisms of Disease

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Tumor grafts derived from women with breast cancer authentically reflect tumor pathology, growth, metastasis and disease outcomes

(2011) Yoko S DeRose et al.   NATURE MEDICINE

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

(2010) Nick Barker et al.   Cell Stem Cell

Distant metastasis occurs late during the genetic evolution of pancreatic cancer

(2010) Shinichi Yachida et al.   NATURE

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Small molecule–mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

(2009) Baozhi Chen et al.   Nature Chemical Biology

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE

New dimensions in tumor immunology: what does 3D culture reveal?

(2008) Chantal Feder-Mengus et al.   TRENDS IN MOLECULAR MEDICINE