Biophysical studies of phase separation integrating experimental and computational methods

Alteration of Microstructure in Biopolymeric Hydrogels via Compositional Modification of Resilin-Like Polypeptides

(2021) Cristobal Garcia Garcia et al.   ACS Biomaterials-Science & Engineering

A predictive coarse-grained model for position-specific effects of post-translational modifications

(2021) Theodora Myrto Perdikari et al.   BIOPHYSICAL JOURNAL

Real-time observation of structure and dynamics during the liquid-to-solid transition of FUS LC

(2021) Raymond F. Berkeley et al.   BIOPHYSICAL JOURNAL

NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation

(2021) Leonidas Emmanouilidis et al.   Nature Chemical Biology

Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions

(2021) Georg Krainer et al.   Nature Communications

Physics-based computational and theoretical approaches to intrinsically disordered proteins

(2021) Joan-Emma Shea et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation

(2021) Ryan W. Tibble et al.   Nature Chemical Biology

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

The (un)structural biology of biomolecular liquid-liquid phase separation using NMR spectroscopy

(2020) Anastasia C. Murthy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

Biomolecular Phase Separation: From Molecular Driving Forces to Macroscopic Properties

(2020) Gregory L. Dignon et al.   Annual Review of Physical Chemistry

Identifying sequence perturbations to an intrinsically disordered protein that determine its phase-separation behavior

(2020) Benjamin S. Schuster et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

Biomolecular condensates undergo a generic shear-mediated liquid-to-solid transition

(2020) Yi Shen et al.   Nature Nanotechnology

Phase transition of RNA−protein complexes into ordered hollow condensates

(2020) Ibraheem Alshareedah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Unraveling Molecular Interactions in Liquid–Liquid Phase Separation of Disordered Proteins by Atomistic Simulations

(2020) Matteo Paloni et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Refining All-Atom Protein Force Fields for Polar-Rich, Prion-like, Low-Complexity Intrinsically Disordered Proteins

(2020) Wai Shing Tang et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Conformational Ensembles of an Intrinsically Disordered Protein Consistent with NMR, SAXS, and Single-Molecule FRET

(2020) Gregory-Neal W. Gomes et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The effects of cosolutes and crowding on the kinetics of protein condensate formation based on liquid–liquid phase separation: a pressure-jump relaxation study

(2020) Hasan Cinar et al.   Scientific Reports

Simulation of FUS Protein Condensates with an Adapted Coarse-Grained Model

(2020) Zakarya Benayad et al.   Journal of Chemical Theory and Computation

Molecular Details of Protein Condensates Probed by Microsecond Long Atomistic Simulations

(2020) Wenwei Zheng et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation

(2019) Brian Tsang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Simulation methods for liquid–liquid phase separation of disordered proteins

(2019) Gregory L Dignon et al.   Current Opinion in Chemical Engineering

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Lysine/RNA-interactions drive and regulate biomolecular condensation

(2019) Tina Ukmar-Godec et al.   Nature Communications

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

Atomic-level insight into mRNA processing bodies by combining solid and solution-state NMR spectroscopy

(2019) Reinier Damman et al.   Nature Communications

NPM1 exhibits structural and dynamic heterogeneity upon phase separation with the p14ARF tumor suppressor

(2019) Eric Gibbs et al.   JOURNAL OF MAGNETIC RESONANCE

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

Probing Conformational Exchange in Weakly Interacting, Slowly Exchanging Protein Systems via Off-Resonance R1ρ Experiments: Application to Studies of Protein Phase Separation

(2018) Tairan Yuwen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Genetically encoded lipid–polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly

(2018) Davoud Mozhdehi et al.   Nature Chemistry

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Sequence determinants of protein phase behavior from a coarse-grained model

(2018) Gregory L. Dignon et al.   PLoS Computational Biology

Microstructured Elastomer-PEG Hydrogels via Kinetic Capture of Aqueous Liquid-Liquid Phase Separation

(2018) Hang Kuen Lau et al.   Advanced Science

Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles

(2018) Diana M. Mitrea et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular Dynamics Simulation for All

(2018) Scott A. Hollingsworth et al.   NEURON

Improvements to the AMOEBA Force Field by Introducing Anisotropic Atomic Polarizability of the Water Molecule

(2018) Akshaya K. Das et al.   Journal of Chemical Theory and Computation

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles

(2017) Ming-Tzo Wei et al.   Nature Chemistry

A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping

(2017) Stefan Schütz et al.   NUCLEIC ACIDS RESEARCH

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

The liquid structure of elastin

(2017) Sarah Rauscher et al.   eLife

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins

(2017) Tyler S Harmon et al.   eLife

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA

(2016) Diana M Mitrea et al.   eLife

Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors

(2015) Sigrid Milles et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

The tip of the iceberg: RNA-binding proteins with prion-like domains in neurodegenerative disease

(2012) Oliver D. King et al.   BRAIN RESEARCH

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL