Methodologies in visualizing the activation of CRISPR/Cas: The last mile in developing CRISPR-Based diagnostics and biosensing – A review

Holographic Optical Tweezers and Boosting Upconversion Luminescent Resonance Energy Transfer Combined Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a Biosensors

(2021) Cheng-Yu Li et al.   ACS Nano

Reprogrammable Gel Electrophoresis Detection Assay Using CRISPR-Cas12a and Hybridization Chain Reaction

(2021) Mahera J. Kachwala et al.   ANALYTICAL CHEMISTRY

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a for the Development of a Mxene Based Electrochemiluminescence Biosensor for the Detection of Siglec-5

(2021) Kai Zhang et al.   BIOSENSORS & BIOELECTRONICS

A CRISPR‐Cas12a‐Derived Biosensor Enabling Portable Personal Glucose Meter Readout for Quantitative Detection of SARS‐CoV‐2

(2021) Di Huang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Exploring the Trans-Cleavage Activity of CRISPR/Cas12a on Gold Nanoparticles for Stable and Sensitive Biosensing

(2021) Xiaoyi Fu et al.   ANALYTICAL CHEMISTRY

Signal-Amplified Detection of the Tumor Biomarker FEN1 Based on Cleavage-Induced Ligation of a Dumbbell DNA Probe and Rolling Circle Amplification

(2021) Bingzhi Li et al.   ANALYTICAL CHEMISTRY

MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

(2021) Anzhi Sheng et al.   ANALYTICAL CHEMISTRY

Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection

(2021) Peng-Fei Liu et al.   BIOSENSORS & BIOELECTRONICS

CRISPR‐Cas12a System for Biosensing and Gene Regulation

(2021) Yuyan Shi et al.   Chemistry-An Asian Journal

Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/Nanotechnologies

(2021) Huahua Yue et al.   ACS Nano

Application of CRISPR/Cas System in the Metabolic Engineering of Small Molecules

(2021) Rajveer Singh et al.   MOLECULAR BIOTECHNOLOGY

CRISPR technology for abiotic stress resistant crop breeding

(2021) Lingling Ma et al.   PLANT GROWTH REGULATION

CRISPR-Cas systems: From gene scissors to programmable biosensors

(2021) Yuxin Zhang et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Nanotechnology-based delivery of CRISPR/Cas9 for cancer treatment

(2021) Xiaoyu Xu et al.   ADVANCED DRUG DELIVERY REVIEWS

An exonuclease protection and CRISPR/Cas12a integrated biosensor for the turn-on detection of transcription factors in cancer cells

(2021) Bingzhi Li et al.   ANALYTICA CHIMICA ACTA

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

(2021) Si-Yuan Wang et al.   ANALYTICA CHIMICA ACTA

Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes – A review

(2021) Siying Xie et al.   ANALYTICA CHIMICA ACTA

G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection

(2021) Tao Li et al.   BIOSENSORS & BIOELECTRONICS

Recent advancements in CRISPR-Cas toolbox for imaging applications

(2021) Vikram Singh et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

CRISPR/Cas9: Principle, Applications, and Delivery through Extracellular Vesicles

(2021) Katarzyna Horodecka et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Versatile modification of the CRISPR/Cas9 ribonucleoprotein system to facilitate in vivo application

(2021) Bixi Sun et al.   JOURNAL OF CONTROLLED RELEASE

CRISPR/Cas Advancement in Molecular Diagnostics and Signal Readout Approaches

(2021) Md Z. Ahmed et al.   JOURNAL OF MOLECULAR DIAGNOSTICS

Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

(2021) Huahua Yue et al.   NANO LETTERS

Wearable materials with embedded synthetic biology sensors for biomolecule detection

(2021) Peter Q. Nguyen et al.   NATURE BIOTECHNOLOGY

Accelerated RNA detection using tandem CRISPR nucleases

(2021) Tina Y. Liu et al.   Nature Chemical Biology

Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter

(2021) Christopher W. Smith et al.   ACS Synthetic Biology

Challenges and Opportunities for Clustered Regularly Interspaced Short Palindromic Repeats Based Molecular Biosensing

(2021) Mengdi Bao et al.   ACS Sensors

CRISPR-based diagnostics

(2021) Michael M. Kaminski et al.   Nature Biomedical Engineering

Applications of CRISPR as a potential therapeutic

(2021) Ritika Luthra et al.   LIFE SCIENCES

Single-stranded circular DNA theranostics

(2021) Tingting Shen et al.   Theranostics

Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

(2020) Jinjin Shen et al.   Nature Communications

Surpassing the detection limit and accuracy of the electrochemical DNA sensor through the application of CRISPR Cas systems

(2020) Wei Xu et al.   BIOSENSORS & BIOELECTRONICS

Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics

(2020) Menglu Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

(2020) Carmela Garcia-Doval et al.   Nature Communications

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

(2020) Shuhan Lu et al.   Cell Discovery

CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point‐of‐Care Diagnosis

(2020) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPR-Cas9 Applications in Cardiovascular Disease

(2020) John Paul S. Khouzam et al.   CURRENT PROBLEMS IN CARDIOLOGY

Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains

(2020) Dalia Smalakyte et al.   NUCLEIC ACIDS RESEARCH

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

(2020) Chantal B. F. Vogels et al.   Nature Microbiology

An Integrated Multi‐Function Heterogeneous Biochemical Circuit for High‐Resolution Electrochemistry‐Based Genetic Analysis

(2020) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A CRISPR-derived biosensor for the sensitive detection of transcription factors based on the target-induced inhibition of Cas12a activation

(2020) Bingzhi Li et al.   BIOSENSORS & BIOELECTRONICS

Genome Editing: Small-Size CasΦ Is Shining

(2020) Xingliang Ma et al.   Molecular Plant

Simultaneous Dual‐Gene Diagnosis of SARS‐CoV‐2 Based on CRISPR/Cas9‐Mediated Lateral Flow Assay

(2020) Erhu Xiong et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A CRISPR-driven colorimetric code platform for highly accurate telomerase activity assay

(2020) Meng Cheng et al.   BIOSENSORS & BIOELECTRONICS

Terminal deoxynucleotidyl transferase combined CRISPR-Cas12a amplification strategy for ultrasensitive detection of uracil-DNA glycosylase with zero background

(2020) Yi-Chen Du et al.   BIOSENSORS & BIOELECTRONICS

Structure of the miniature type V-F CRISPR-Cas effector enzyme

(2020) Satoru N. Takeda et al.   MOLECULAR CELL

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

(2020) Jin-Ha Choi et al.   NANO LETTERS

Digital CRISPR/Cas‐Assisted Assay for Rapid and Sensitive Detection of SARS‐CoV‐2

(2020) Joon Soo Park et al.   Advanced Science

Rational design, synthesis, and applications of carbon dots@metal–organic frameworks (CD@MOF) based sensors

(2020) Bingzhi Li et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor

(2020) Ruijin Zeng et al.   Nano Energy

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification

(2019) Weidan Chang et al.   MICROCHIMICA ACTA

Cas14: Big Advances from Small CRISPR Proteins

(2019) David F. Savage   BIOCHEMISTRY

CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic

(2019) Guillermo Aquino-Jarquin   Nanomedicine-Nanotechnology Biology and Medicine

The Exploration of a New Stable G-Triplex DNA and its Novel Function in Electrochemical Biosensing

(2019) Ling-Li Zhao et al.   ANALYTICAL CHEMISTRY

Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay

(2019) Yongya Li et al.   ANALYTICAL CHEMISTRY

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rolling Circular Amplification (RCA)-Assisted CRISPR/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs

(2019) Ruixuan Wang et al.   ANALYTICAL CHEMISTRY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Luminescence resonance energy transfer spectroscopy of ATP-binding cassette proteins

(2018) Maria E. Zoghbi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases

(2018) Bonhan Koo et al.   SENSORS AND ACTUATORS B-CHEMICAL

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Discovery of widespread Type I and Type V CRISPR-Cas inhibitors

(2018) Nicole D. Marino et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme

(2017) Gavin J Knott et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme

(2017) Gavin J Knott et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The chemistry of Cas9 and its CRISPR colleagues

(2017) Janice S. Chen et al.   Nature Reviews Chemistry

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition

(2016) Pu Gao et al.   CELL RESEARCH

731. High-Fidelity CRISPR-Cas9 Nucleases with No Detectable Genome-Wide Off-Target Effects

(2016) Benjamin P. Kleinstiver et al.   MOLECULAR THERAPY

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

(2014) Y. Lin et al.   NUCLEIC ACIDS RESEARCH

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE