An Overview of Cytochrome P450 Immobilization Strategies for Drug Metabolism Studies, Biosensing, and Biocatalytic Applications: Challenges and Opportunities

Materials and Methods of Biosensor Interfaces With Stability

(2021) Min Song et al.   Frontiers in Materials

Faster Surface Ligation Reactions Improve Immobilized Enzyme Structure and Activity

(2021) Andres F. Chaparro Sosa et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Process intensification for cytochrome P450 BM3‐catalyzed oxy‐functionalization of dodecanoic acid

(2020) Moritz B. Buergler et al.   BIOTECHNOLOGY AND BIOENGINEERING

Surface charge-controlled electron transfer and catalytic behavior of immobilized cytochrome P450 BM3 inside dendritic mesoporous silica nanoparticles

(2020) Qumei Dai et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?

(2020) Sara Arana-Peña et al.   BIOTECHNOLOGY ADVANCES

On the taught new tricks of enzymes immobilization: An all-inclusive overview

(2020) Roswanira Abdul Wahab et al.   REACTIVE & FUNCTIONAL POLYMERS

MicroGelzymes: pH-Independent Immobilization of Cytochrome P450 BM3 in Microgels

(2020) Maximilian Nöth et al.   BIOMACROMOLECULES

Immobilized Enzymes in Biosensor Applications

(2019) Hoang Nguyen et al.   Materials

Cytochrome P450 Monooxygenases in Biotechnology and Synthetic Biology

(2019) Vlada B. Urlacher et al.   TRENDS IN BIOTECHNOLOGY

Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization

(2019) Ee Taek Hwang et al.   ACS Catalysis

Preparation of cytochrome P450 enzyme-cobalt phosphate hybrid nano-flowers for oxidative coupling of benzylamine

(2019) Kui Wu et al.   ENZYME AND MICROBIAL TECHNOLOGY

One-pot Two-Step Chemoenzymatic Cascade for the Synthesis of a Bis-benzofuran Derivative

(2019) M.A. Stephanie Mertens et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Directed Evolution of P450 BM3 towards Functionalization of Aromatic O-Heterocycles

(2019) Gustavo de Almeida Santos et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Immobilization of P450 BM3 monooxygenase on hollow nanosphere composite: Application for degradation of organic gases pollutants under solar radiation lamp

(2019) Gamal Awad et al.   APPLIED CATALYSIS B-ENVIRONMENTAL

The Microenvironment in Immobilized Enzymes: Methods of Characterization and Its Role in Determining Enzyme Performance

(2019) Juan M. Bolivar et al.   MOLECULES

Engineered enzymes that retain and regenerate their cofactors enable continuous-flow biocatalysis

(2019) Carol J. Hartley et al.   Nature Catalysis

Paper-based sol-gel thin films immobilized cytochrome P450 for enzyme activity measurement

(2019) Nantana Nuchtavorn et al.   ANALYTICA CHIMICA ACTA

Selective Functionalization of Microgels with Enzymes by Sortagging

(2019) Zhi Zou et al.   BIOCONJUGATE CHEMISTRY

Recent progress in multienzymes co-immobilization and multienzyme system applications

(2019) Sizhu Ren et al.   CHEMICAL ENGINEERING JOURNAL

On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

(2019) Juan M. Bolivar et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Industrial applications of immobilized enzymes—A review

(2019) Alessandra Basso et al.   Molecular Catalysis

Covalent immobilization of cytochrome P450 BM3 (R966D/W1046S) on glutaraldehyde activated SPIONs

(2018) Atieh Bahrami et al.   CANADIAN JOURNAL OF CHEMICAL ENGINEERING

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

(2018) Ana I. Benítez-Mateos et al.   ChemCatChem

Surface-Binding Peptide Facilitates Electricity-Driven NADPH-Free Cytochrome P450 Catalysis

(2018) Sarah Zernia et al.   ChemCatChem

Recent developments in the application of P450 based biocatalysts

(2018) Yifeng Wei et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Improved electrocatalytic metabolite production and drug biosensing by human liver microsomes immobilized on amine-functionalized magnetic nanoparticles

(2018) Rajasekhara Nerimetla et al.   ELECTROCHIMICA ACTA

Liver ‘organ on a chip’

(2018) Colin H. Beckwitt et al.   EXPERIMENTAL CELL RESEARCH

Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag™ fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol

(2018) J. Döbber et al.   GREEN CHEMISTRY

Co-immobilization of P450 BM3 and Glucose Dehydrogenase on different supports for application as a self-sufficient oxidative biocatalyst

(2018) Jordi Solé et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Membrane-attached mammalian cytochromes P450: An overview of the membrane's effects on structure, drug binding, and interactions with redox partners

(2018) Martin Šrejber et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Cross-linked cytochrome P450 BM3 aggregates promoted by Ru(II)-diimine complexes bearing aldehyde groups

(2018) Minh Quan Do et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Effect of immobilization site on the orientation and activity of surface-tethered enzymes

(2018) Yaoxin Li et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

(2018) Jakub Zdarta et al.   Catalysts

Digital microfluidic immobilized cytochrome P450 reactors with integrated inkjet-printed microheaters for droplet-based drug metabolism research

(2018) Gowtham Sathyanarayanan et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Polydopamine functionalized nanoporous graphene foam as nanoreactor for efficient electrode-driven metabolism of steroid hormones

(2018) Xuan Xu et al.   BIOSENSORS & BIOELECTRONICS

From electrochemistry to enzyme kinetics of cytochrome P450

(2018) Victoria V. Shumyantseva et al.   BIOSENSORS & BIOELECTRONICS

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

(2018) Donya Valikhani et al.   BIOTECHNOLOGY AND BIOENGINEERING

Magnetic nanoparticles as versatile carriers for enzymes immobilization: A review

(2018) Muhammad Bilal et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Influence of inter-domain dynamics and surrounding environment flexibility on the direct electrochemistry and electrocatalysis of self-sufficient cytochrome P450 3A4-BMR chimeras

(2018) Silvia Castrignanò et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Development of quantum dot-based biosensors: principles and applications

(2018) Fei Ma et al.   Journal of Materials Chemistry B

A genetically-encoded synthetic self-assembled multienzyme complex of lipase and P450 fatty acid decarboxylase for efficient bioproduction of fatty alkenes

(2018) Fei Li et al.   BIORESOURCE TECHNOLOGY

Immobilized Enzyme Reactors: an Overview of Applications in Drug Discovery from 2008 to 2018

(2018) Angela De Simone et al.   CHROMATOGRAPHIA

Multi-point enzyme immobilization, surface chemistry, and novel platforms: a paradigm shift in biocatalyst design

(2018) Muhammad Bilal et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

A generic platform for the immobilisation of engineered biocatalysts

(2018) Matthew P. Thompson et al.   TETRAHEDRON

Mechanisms of Cytochrome P450-Catalyzed Oxidations

(2018) F. Peter Guengerich   ACS Catalysis

Noncovalent Immobilization of Optimized Bacterial Cytochrome P450 BM3 on Functionalized Magnetic Nanoparticles

(2017) Atieh Bahrami et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Immobilization of Enzymes in Mesoporous Silica Particles: Protein Concentration and Rotational Mobility in the Pores

(2017) Pegah S. Nabavi Zadeh et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality

(2017) Ross D. Milton et al.   Journal of the Royal Society Interface

Oriented Coimmobilization of Oxidase and Catalase on Tailor-Made Ordered Mesoporous Silica

(2017) Juan M. Bolivar et al.   LANGMUIR

Expansion of chemical space for natural products by uncommon P450 reactions

(2017) Xingwang Zhang et al.   NATURAL PRODUCT REPORTS

Cofactor NAD(P)H Regeneration Inspired by Heterogeneous Pathways

(2017) Xiaodong Wang et al.   Chem

Polydopamine Grafted Porous Graphene as Biocompatible Nanoreactor for Efficient Identification of Membrane Proteins

(2016) Xiaoni Fang et al.   ACS Applied Materials & Interfaces

Immobilization of a Bacterial Cytochrome P450 Monooxygenase System on a Solid Support

(2016) Cheau Yuaan Tan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes

(2016) Susana Velasco-Lozano et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Phasins, Multifaceted Polyhydroxyalkanoate Granule-Associated Proteins

(2016) Mariela P. Mezzina et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Peptide-Mediated Specific Immobilization of Catalytically Active Cytochrome P450 BM3 Variant

(2016) Sarah Zernia et al.   BIOCONJUGATE CHEMISTRY

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

(2016) Juan M. Bolivar et al.   CATALYSIS TODAY

Expanding an Efficient, Electrically Driven and CNT-Tagged P450 System into the Third Dimension: A Nanowired CNT-Containing and Enzyme-Stabilising 3 D Sol-Gel Electrode

(2016) Enrique C. Llaudet et al.   CHEMBIOCHEM

Liver and Kidney on Chips: Microphysiological Models to Understand Transporter Function

(2016) SY Chang et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

(2016) Jochen Wachtmeister et al.   CURRENT OPINION IN BIOTECHNOLOGY

Process development for oxidations of hydrophobic compounds applying cytochrome P450 monooxygenases in-vitro

(2016) Jan Brummund et al.   JOURNAL OF BIOTECHNOLOGY

Direct electrochemistry and electrocatalysis of cytochrome P450s immobilized on gold/graphene-based nanocomposites

(2016) Li Shangguan et al.   JOURNAL OF ELECTROANALYTICAL CHEMISTRY

Confining nanohybrid of CdTe quantum dots and cytochrome P450 2D6 in macroporous ordered siliceous foam for drug metabolism

(2016) Ling Jiang et al.   JOURNAL OF ELECTROANALYTICAL CHEMISTRY

Solar-to-chemical conversion platform by Robust Cytochrome P450-P(3HB) complex

(2016) Jae Hyung Lee et al.   JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY

Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli

(2016) Paul Quehl et al.   Microbial Cell Factories

Enabling Selective and Sustainable P450 Oxygenation Technology. Production of 4-Hydroxy-α-isophorone on Kilogram Scale

(2016) Iwona Kaluzna et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Immobilized multienzymatic systems for catalysis of cascade reactions

(2016) Qingzhi Ji et al.   PROCESS BIOCHEMISTRY

“Cells-on-Beads”: A novel immobilization approach for the construction of whole-cell amperometric biosensors

(2016) Tal Yoetz-Kopelman et al.   SENSORS AND ACTUATORS B-CHEMICAL

Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

(2015) Xu-Dong Wang et al.   ADVANCED MATERIALS

HaloTag Technology: A Versatile Platform for Biomedical Applications

(2015) Christopher G. England et al.   BIOCONJUGATE CHEMISTRY

Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation

(2015) Elena Cambria et al.   BIOMACROMOLECULES

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

(2015) Oveimar Barbosa et al.   BIOTECHNOLOGY ADVANCES

Importance of the Support Properties for Immobilization or Purification of Enzymes

(2015) Jose Cleiton S. dos Santos et al.   ChemCatChem

Electrocatalysis by subcellular liver fractions bound to carbon nanostructures for stereoselective green drug metabolite synthesis

(2015) Rajasekhara Nerimetla et al.   CHEMICAL COMMUNICATIONS

Cytochrome P450 bienzymes assembled on Au/chitosan/reduced graphene oxide nanosheets for electrochemically-driven drug cascade metabolism

(2015) Jusheng Lu et al.   ELECTROCHIMICA ACTA

Oriented immobilization of proteins on solid supports for use in biosensors and biochips: a review

(2015) Yingshuai Liu et al.   MICROCHIMICA ACTA

Solid-binding peptides: smart tools for nanobiotechnology

(2015) Andrew Care et al.   TRENDS IN BIOTECHNOLOGY

Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst

(2015) Juan M. Bolivar et al.   ACS Catalysis

Supramolecular protein assembly supports immobilization of a cytochrome P450 monooxygenase system as water-insoluble gel

(2015) Cheau Yuaan Tan et al.   Scientific Reports

Enzyme immobilization by adsorption: a review

(2014) Teofil Jesionowski et al.   ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY

Cytochromes P450 as promising catalysts for biotechnological application: chances and limitations

(2014) Rita Bernhardt et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

New Platform for Cytochrome P450 Reaction Combining in Situ Immobilization on Biopolymer

(2014) Jae Hyung Lee et al.   BIOCONJUGATE CHEMISTRY

Survey of Human Oxidoreductases and Cytochrome P450 Enzymes Involved in the Metabolism of Xenobiotic and Natural Chemicals

(2014) Slobodan Rendic et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy

(2014) Markus Ritzefeld   CHEMISTRY-A EUROPEAN JOURNAL

Expanding P450 catalytic reaction space through evolution and engineering

(2014) John A McIntosh et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Cytochrome P450 Modified Polycrystalline Indium Tin Oxide Film as a Drug Metabolizing Electrochemical Biosensor with a Simple Configuration

(2013) Kyoko Yoshioka et al.   ANALYTICAL CHEMISTRY

The Use of Immobilized Cytochrome P4502C9 in PMMA-Based Plug Flow Bioreactors for the Production of Drug Metabolites

(2013) Lance A. Wollenberg et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Streptavidin–biotin technology: improvements and innovations in chemical and biological applications

(2013) Christopher M. Dundas et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Encapsulation of Enzymes in Layer-by-Layer (LbL) Structures: Latest Advances and Applications

(2013) Omar. S. Sakr et al.   BIOMACROMOLECULES

Co-immobilized coupled enzyme systems in biotechnology

(2013) Lorena Betancor et al.   Biotechnology & Genetic Engineering Reviews

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods

(2013) Sara Cantone et al.   CHEMICAL SOCIETY REVIEWS

Evaluation of immobilized enzymes for industrial applications

(2013) Andreas Liese et al.   CHEMICAL SOCIETY REVIEWS

Conformational changes of enzymes upon immobilisation

(2013) Francesco Secundo   CHEMICAL SOCIETY REVIEWS

Coupling Photocatalysis and Redox Biocatalysis Toward Biocatalyzed Artificial Photosynthesis

(2013) Sahng Ha Lee et al.   CHEMISTRY-A EUROPEAN JOURNAL

Sensors based on cytochrome P450 and CYP mimicking systems

(2013) Aysu Yarman et al.   ELECTROCHIMICA ACTA

Fabrication and characterization of microfluidic liver-on-a-chip using microsomal enzymes

(2013) Jungwoo Lee et al.   ENZYME AND MICROBIAL TECHNOLOGY

Benzylic hydroxylation of aromatic compounds by P450 BM3

(2013) Katharina Neufeld et al.   GREEN CHEMISTRY

Cytochrome P450 enzymes in drug metabolism: Regulation of gene expression, enzyme activities, and impact of genetic variation

(2013) Ulrich M. Zanger et al.   PHARMACOLOGY & THERAPEUTICS

Cytochrome P450 (CYP) enzymes and the development of CYP biosensors

(2012) Elizabeth Schneider et al.   BIOSENSORS & BIOELECTRONICS

Modifying enzyme activity and selectivity by immobilization

(2012) Rafael C. Rodrigues et al.   CHEMICAL SOCIETY REVIEWS

Electrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes

(2012) Camilla Baj-Rossi et al.   SENSORS

Effects of Microenvironment on Supported Enzymes

(2012) Fei Gao et al.   TOPICS IN CATALYSIS

Enzyme immobilization: an overview on techniques and support materials

(2012) Sumitra Datta et al.   3 Biotech

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Cell-based electrochemical biosensors for water quality assessment

(2011) Florence Lagarde et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Enzyme-Based Amperometric Platform to Determine the Polymorphic Response in Drug Metabolism by Cytochromes P450

(2011) Paola Panicco et al.   ANALYTICAL CHEMISTRY

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

(2011) Roger A. Sheldon   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

First steps towards a Zn/Co(III)sep-driven P450 BM3 reactor

(2011) Liqing Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enzymes immobilized on carbon nanotubes

(2011) Wei Feng et al.   BIOTECHNOLOGY ADVANCES

Oriented Immobilization of Enzymes Made Fit for Applied Biocatalysis: Non-Covalent Attachment to Anionic Supports usingZbasic2Module

(2011) Johanna Wiesbauer et al.   ChemCatChem

Cytochromes P450 as useful biocatalysts: addressing the limitations

(2011) Elaine O'Reilly et al.   CHEMICAL COMMUNICATIONS

P450BM3(CYP102A1): connecting the dots

(2011) Christopher J. C. Whitehouse et al.   CHEMICAL SOCIETY REVIEWS

3-Mercaptopropionic acid capped ZnSe quantum dot-cytochrome P450 3A4 enzyme biotransducer for 17β-estradiol

(2011) Peter M. Ndangili et al.   JOURNAL OF ELECTROANALYTICAL CHEMISTRY

Evaluation of an in-capillary approach for performing quantitative cytochrome P450 activity studies

(2010) R. Curcio et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Control of Human Cytochrome P450 2E1 Electrocatalytic Response as a Result of Unique Orientation on Gold Electrodes

(2010) Lok Hang Mak et al.   ANALYTICAL CHEMISTRY

Breakthrough in P450 bioelectrochemistry and future perspectives

(2010) Sheila J. Sadeghi et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Molecular Assembly of P450 with Ferredoxin and Ferredoxin Reductase by Fusion to PCNA

(2010) Hidehiko Hirakawa et al.   CHEMBIOCHEM

Engineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediation

(2010) Santosh Kumar   Expert Opinion on Drug Metabolism & Toxicology

Immobilization of P450 BM-3 monooxygenase on mesoporous molecular sieves with different pore diameters

(2010) Evelyne Weber et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Cross-Linked Enzyme Aggregates as Industrial Biocatalysts

(2010) Roger A. Sheldon   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering

(2009) Shinya Tsukiji et al.   CHEMBIOCHEM

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Understanding enzyme immobilisation

(2008) Ulf Hanefeld et al.   CHEMICAL SOCIETY REVIEWS