Importance of the Support Properties for Immobilization or Purification of Enzymes

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

(2015) Oveimar Barbosa et al.   BIOTECHNOLOGY ADVANCES

Selective biomineralization of Co3(PO4)2-sponges triggered by His-tagged proteins: efficient heterogeneous biocatalysts for redox processes

(2015) Fernando López-Gallego et al.   CHEMICAL COMMUNICATIONS

Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

(2015) Evelin A. Manoel et al.   ENZYME AND MICROBIAL TECHNOLOGY

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment

(2015) Jose C.S. dos Santos et al.   ENZYME AND MICROBIAL TECHNOLOGY

Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachment

(2015) Jose C.S. dos Santos et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Immobilization of lipases on glyoxyl–octyl supports: Improved stability and reactivation strategies

(2015) Angélica Suescun et al.   PROCESS BIOCHEMISTRY

Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties

(2015) Jose C.S. dos Santos et al.   PROCESS BIOCHEMISTRY

Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization

(2015) Jose C. S. dos Santos et al.   RSC Advances

Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

(2015) Jose C. S. dos Santos et al.   RSC Advances

Biosensors Based on Porous Cellulose Nanocrystal–Poly(vinyl Alcohol) Scaffolds

(2014) Bastien Schyrr et al.   ACS Applied Materials & Interfaces

Selective oxidation of glycerol to 1,3-dihydroxyacetone by covalently immobilized glycerol dehydrogenases with higher stability and lower product inhibition

(2014) Javier Rocha-Martin et al.   BIORESOURCE TECHNOLOGY

Biocatalysis as a Strategic Green Technology for the Chemical Industry

(2014) Andy Wells et al.   ChemCatChem

Immobilized Hydroxynitrile Lyase: A Comparative Study of Recyclability

(2014) Guzman Torrelo et al.   ChemCatChem

Dye decolorisation by laccase immobilised in lens-shaped poly(vinyl alcohol) hydrogel capsules

(2014) Radek Stloukal et al.   CHEMICAL PAPERS

Combining the ‘two worlds’ of chemocatalysis and biocatalysis towards multi-step one-pot processes in aqueous media

(2014) Harald Gröger et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering aldolases as biocatalysts

(2014) Claire L Windle et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Immobilized phospholipase A1-catalyzed modification of phosphatidylcholine with n−3 polyunsaturated fatty acid

(2014) TingTing Zhao et al.   FOOD CHEMISTRY

Pore Size – a Key Property for Selective Toxin Removal in Blood Purification

(2014) Stephan Harm et al.   INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS

Evaluation of kinetic parameters of immobilized penicillin G acylase subject to an inactivation and reactivation process

(2014) Oscar Romero et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Reversible, selective immobilization of nuclease P1 from a crude enzyme solution on a weak base anion resin activated by polyethylenimine

(2014) Bingbing Li et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Synthesis of Novel Magnetic Cellulose-Chitosan Composite Microspheres and Their Application in Laccase Immobilization

(2014) Shuai Peng et al.   JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY

Heterofunctional Hydrophilic–Hydrophobic Porous Silica as Support for Multipoint Covalent Immobilization of Lipases: Application to Lactulose Palmitate Synthesis

(2014) Claudia Bernal et al.   LANGMUIR

Inorganic Materials as Supports for Covalent Enzyme Immobilization: Methods and Mechanisms

(2014) Paolo Zucca et al.   MOLECULES

Additives Enhancing the Catalytic Properties of Lipase from Burkholderia cepacia Immobilized on Mixed-Function-Grafted Mesoporous Silica Gel

(2014) Emese Abaházi et al.   MOLECULES

Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases

(2014) Cristina Garcia-Galan et al.   MOLECULES

Negative chromatography: Progress, applications and future perspectives

(2014) Micky Fu Xiang Lee et al.   PROCESS BIOCHEMISTRY

Biocatalysis combined with physical technologies for development of a green biodiesel process

(2014) Camilo Barroso Teixeira et al.   RENEWABLE & SUSTAINABLE ENERGY REVIEWS

Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

(2014) Rafael C. Rodrigues et al.   RSC Advances

Rapid Flow-Through Biocatalysis with High Surface Area, Enzyme-Loaded Carbon and Gold-Bearing Diatom Frustule Replicas

(2013) Stan C. Davis et al.   ADVANCED FUNCTIONAL MATERIALS

Enzymes immobilized in mesoporous silica: A physical–chemical perspective

(2013) Nils Carlsson et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Internal and external mass transfer limitations on the activity of immobilised acid urease derivatives differing in enzyme loading

(2013) Nadia Bortone et al.   BIOCHEMICAL ENGINEERING JOURNAL

Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

(2013) Oveimar Barbosa et al.   BIOMACROMOLECULES

Vinyl sulfone silica: application of an open preactivated support to the study of transnitrosylation of plant proteins by S-nitrosoglutathione

(2013) Juan C Begara-Morales et al.   BMC PLANT BIOLOGY

Enhanced activity toward PET by site-directed mutagenesis of Thermobifida fusca cutinase–CBM fusion protein

(2013) Yao Zhang et al.   CARBOHYDRATE POLYMERS

Microreactors and CFD as Tools for Biocatalysis Reactor Design: A case study

(2013) V. K. Bodla et al.   CHEMICAL ENGINEERING & TECHNOLOGY

Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification

(2013) Jian Luo et al.   CHEMICAL ENGINEERING SCIENCE

Industrial use of immobilized enzymes

(2013) Robert DiCosimo et al.   CHEMICAL SOCIETY REVIEWS

Immobilization of enzymes on porous silicas – benefits and challenges

(2013) Martin Hartmann et al.   CHEMICAL SOCIETY REVIEWS

Protein engineering of enzymes for process applications

(2013) John M Woodley   CURRENT OPINION IN CHEMICAL BIOLOGY

Using site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes

(2013) Forest H. Andrews et al.   FEBS Journal

The study on effective immobilization of lipase on functionalized bentonites and their properties

(2013) Huaping Dong et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Immobilization of pectinase on polyethyleneimine-coated pulp fiber for treatment of whitewater from papermaking

(2013) Rina Wu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Current status and trends in enzymatic nanoimmobilization

(2013) Eliane P. Cipolatti et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Modulation of the Microenvironment Surrounding the Active Site of Penicillin G Acylase Immobilized on Acrylic Carriers Improves the Enzymatic Synthesis of Cephalosporins

(2013) Paolo Bonomi et al.   MOLECULES

Strategies for Stabilization of Enzymes in Organic Solvents

(2013) Veronika Stepankova et al.   ACS Catalysis

Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

(2013) Oveimar Barbosa et al.   RSC Advances

Magnetic Bead Cellulose as a Suitable Support for Immobilization of α-Chymotrypsin

(2012) Petr Prikryl et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Immobilized metal ion affinity chromatography: a review on its applications

(2012) Randy Chi Fai Cheung et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Vinyl Sulfone Functionalization: A Feasible Approach for the Study of the Lectin–Carbohydrate Interactions

(2012) Francisco Javier Lopez-Jaramillo et al.   BIOCONJUGATE CHEMISTRY

Immobilization of Escherichia coli containing ω-transaminase activity in LentiKats®

(2012) Max Cárdenas-Fernández et al.   BIOTECHNOLOGY PROGRESS

Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts

(2012) Caterina Boniello et al.   BMC BIOTECHNOLOGY

Modifying enzyme activity and selectivity by immobilization

(2012) Rafael C. Rodrigues et al.   CHEMICAL SOCIETY REVIEWS

Enabling the utilization of wool as an enzyme support: Enhancing the activity and stability of lipase immobilized onto woolen cloth

(2012) Xudong Feng et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Directed evolution: tailoring biocatalysts for industrial applications

(2012) Ashwani Kumar et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

(2012) Karel Hernandez et al.   CURRENT ORGANIC CHEMISTRY

Enzyme stabilization by nano/microsized hybrid materials

(2012) Ee Taek Hwang et al.   ENGINEERING IN LIFE SCIENCES

Immobilization of horseradish peroxidase onto clay minerals using soil organic matter for phenol removal

(2012) Hyo Jun Kim et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Novel synthetic route for covalent coupling of biomolecules on super-paramagnetic hybrid nanoparticles

(2012) Antonio L. Medina-Castillo et al.   JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica

(2012) Oveimar Barbosa et al.   PROCESS BIOCHEMISTRY

Lipase promiscuity and its biochemical applications

(2012) Manali Kapoor et al.   PROCESS BIOCHEMISTRY

Applications of functionalized and nanoparticle-modified nanocrystalline cellulose

(2012) Edmond Lam et al.   TRENDS IN BIOTECHNOLOGY

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

(2011) Cristina Garcia-Galan et al.   ADVANCED SYNTHESIS & CATALYSIS

Oils and Fats as Renewable Raw Materials in Chemistry

(2011) Ursula Biermann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Effect of Internal Diffusional Restrictions on the Hydrolysis of Penicillin G: Reactor Performance and Specific Productivity of 6-APA with Immobilized Penicillin Acylase

(2011) Pedro Valencia et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers

(2011) Karolina Labus et al.   BIOCHEMICAL ENGINEERING JOURNAL

Poly(styrene–divinylbenzene) beads surface functionalized with di-block polymer grafting and multi-modal ligand attachment: performance of reversibly immobilized lipase in ester synthesis

(2011) Gulay Bayramoglu et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Immobilization of lipase from Candida rugosa on Sepabeads®: the effect of lipase oxidation by periodates

(2011) Nevena Ž. Prlainović et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Potential applications of enzymes immobilized on/in nano materials: A review

(2011) Shakeel Ahmed Ansari et al.   BIOTECHNOLOGY ADVANCES

Soy protein recovery in a solvent-free process using continuous liquid-solid circulating fluidized bed ion exchanger

(2011) Andrew Prince et al.   BIOTECHNOLOGY PROGRESS

Facile One-Step Catalytic Grafting of N-Hydroxysuccinimidyl-Ester-Functionalized Methallylsilane onto Silica for Enzyme Immobilization

(2011) Un-Young Jung et al.   Chemistry-An Asian Journal

Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene–divinylbenzene beads

(2011) Karel Hernandez et al.   ENZYME AND MICROBIAL TECHNOLOGY

Immobilization and stabilization of α-galactosidase on Sepabeads EC-EA and EC-HA

(2011) Hasan Bayraktar et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Immobilization of β-galactosidase from Bacillus circulans onto epoxy-activated acrylic supports

(2011) Pedro Torres et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Integrated One-Pot Enrichment and Immobilization of Styrene Monooxygenase (StyA) Using SEPABEAD EC-EA and EC-Q1A Anion-Exchange Carriers

(2011) Reto Ruinatscha et al.   MOLECULES

Improvement of Enzyme Properties with a Two-Step Immobilizaton Process on Novel Heterofunctional Supports

(2010) Cesar Mateo et al.   BIOMACROMOLECULES

Intraparticle concentration gradients for substrate and acidic product in immobilized cephalosporin C amidase and their dependencies on carrier characteristics and reaction parameters

(2010) Caterina Boniello et al.   BIOTECHNOLOGY AND BIOENGINEERING

Sub-100 nm TiO2mesocrystalline assemblies with mesopores: preparation, characterization, enzyme immobilization and photocatalytic properties

(2010) Pedro Tartaj   CHEMICAL COMMUNICATIONS

Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support

(2010) Jun-Kun Lai et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Evaluation of the incidence of diffusional restrictions on the enzymatic reactions of hydrolysis of penicillin G and synthesis of cephalexin

(2010) Pedro Valencia et al.   ENZYME AND MICROBIAL TECHNOLOGY

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

(2010) Karel Hernandez et al.   ENZYME AND MICROBIAL TECHNOLOGY

Vinyl sulfone functionalized silica: a “ready to use” pre-activated material for immobilization of biomolecules

(2010) Mariano Ortega-Muñoz et al.   JOURNAL OF MATERIALS CHEMISTRY

Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators

(2010) Valeria Miranda et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Cross-Linking of Lipases Adsorbed on Hydrophobic Supports: Highly Selective Hydrolysis of Fish Oil Catalyzed by RML

(2010) Gloria Fernandez-Lorente et al.   JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY

Using of silica particles as porogen for preparation of macroporous chitosan macrospheres suitable for enzyme immobilization

(2010) Sufang Sun et al.   KINETICS AND CATALYSIS

Two step ethanolysis: A simple and efficient way to improve the enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from Thermomyces lanuginosus

(2010) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

Heterofunctional supports for the one-step purification, immobilization and stabilization of large multimeric enzymes: Amino-glyoxyl versus amino-epoxy supports

(2010) Juan M. Bolivar et al.   PROCESS BIOCHEMISTRY

Lipase B from Candida antarctica immobilized on octadecyl Sepabeads: A very stable biocatalyst in the presence of hydrogen peroxide

(2010) Karel Hernandez et al.   PROCESS BIOCHEMISTRY

Recent advances in polymer monoliths for ion-exchange chromatography

(2009) Anna Nordborg et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β-lactam antibiotics

(2009) Oscar Romero et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advances in enzyme immobilisation

(2009) Dean Brady et al.   BIOTECHNOLOGY LETTERS

Magnetically separable bimodal mesoporous carbons with a large capacity for the immobilization of biomolecules

(2009) Marta Sevilla et al.   CARBON

The presence of thiolated compounds allows the immobilization of enzymes on glyoxyl agarose at mild pH values: New strategies of stabilization by multipoint covalent attachment

(2009) Juan M. Bolivar et al.   ENZYME AND MICROBIAL TECHNOLOGY

Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus

(2009) Rafael C. Rodrigues et al.   ENZYME AND MICROBIAL TECHNOLOGY

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

(2009) Roberto Fernandez-Lafuente   ENZYME AND MICROBIAL TECHNOLOGY

Site-directed mutation at residues near the catalytic site of histamine dehydrogenase from Nocardioides simplex and its effects on substrate inhibition

(2009) Maiko Tsutsumi et al.   JOURNAL OF BIOCHEMISTRY

Ligands for mixed-mode protein chromatography: Principles, characteristics and design

(2009) Guofeng Zhao et al.   JOURNAL OF BIOTECHNOLOGY

Review: Microscale methods for high-throughput chromatography development in the pharmaceutical industry

(2009) Sunil Chhatre et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Vinyl sulfone: a versatile function for simple bioconjugation and immobilization

(2009) Julia Morales-Sanfrutos et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Immobilization–stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

(2009) Rafael C. Rodrigues et al.   PROCESS BIOCHEMISTRY

The co-operative effect of physical and covalent protein adsorption on heterofunctional supports

(2009) Juan M. Bolivar et al.   PROCESS BIOCHEMISTRY

Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli

(2009) Valeria Grazú et al.   PROCESS BIOCHEMISTRY

Membrane-based techniques for the separation and purification of proteins: An overview

(2008) Arunima Saxena et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Proteins in Mesoporous Silicates

(2008) Sarah Hudson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

(2008) Juan M. Bolivar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose

(2008) Gloria Fernandez-Lorente et al.   BIOMACROMOLECULES

Practical application of different enzymes immobilized on sepabeads

(2008) L. Hilterhaus et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Understanding enzyme immobilisation

(2008) Ulf Hanefeld et al.   CHEMICAL SOCIETY REVIEWS

Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization

(2008) Juan M. Bolivar et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Mixed-mode hydrophilic interaction/cation-exchange chromatography (HILIC/CEX) of peptides and proteins

(2008) Colin T. Mant et al.   JOURNAL OF SEPARATION SCIENCE

Enzyme stability and stabilization—Aqueous and non-aqueous environment

(2008) Padma V. Iyer et al.   PROCESS BIOCHEMISTRY

Application and properties of butyl acrylate/pentaerythrite triacrylate copolymers and cellulose-based Granocel as carriers for trypsin immobilization

(2007) Jolanta Bryjak et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

One-dimensional crosslinked enzyme aggregates in SBA-15: Superior catalytic behavior to conventional enzyme immobilization

(2007) Moon Il Kim et al.   MICROPOROUS AND MESOPOROUS MATERIALS

New Cationic Exchanger Support for Reversible Immobilization of Proteins

(2004) Manuel Fuentes et al.   BIOTECHNOLOGY PROGRESS