Temperature effects on RNA polymerase initiation kinetics reveal which open complex initiates and that bubble collapse is stepwise

Structural basis of ribosomal RNA transcription regulation

(2021) Yeonoh Shin et al.   Nature Communications

RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription

(2020) Lingting Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescence-Detected Conformational Changes in Duplex DNA in Open Complex Formation by Escherichia coli RNA Polymerase: Upstream Wrapping and Downstream Bending Precede Clamp Opening and Insertion of the Downstream Duplex

(2020) Raashi Sreenivasan et al.   BIOCHEMISTRY

Stepwise Promoter Melting by Bacterial RNA Polymerase

(2020) James Chen et al.   MOLECULAR CELL

XACT-Seq Comprehensively Defines the Promoter-Position and Promoter-Sequence Determinants for Initial-Transcription Pausing

(2020) Jared T. Winkelman et al.   MOLECULAR CELL

RNA Polymerase: Step-by-Step Kinetics and Mechanism of Transcription Initiation

(2019) Kate L. Henderson et al.   BIOCHEMISTRY

Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis

(2019) Laichuang Han et al.   Microbial Cell Factories

CarD and RbpA modify the kinetics of initial transcription and slow promoter escape of the Mycobacterium tuberculosis RNA polymerase

(2019) Drake Jensen et al.   NUCLEIC ACIDS RESEARCH

Correlating Transcription Initiation and Conformational Changes by a Single-Subunit RNA Polymerase with Near Base-Pair Resolution

(2018) Hye Ran Koh et al.   MOLECULAR CELL

Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation

(2018) Robert Glyde et al.   MOLECULAR CELL

DNA template sequence control of bacterial RNA polymerase escape from the promoter

(2018) Ewa Heyduk et al.   NUCLEIC ACIDS RESEARCH

Mechanism of transcription initiation and promoter escape byE.coliRNA polymerase

(2017) Kate L. Henderson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The mechanism of variability in transcription start site selection

(2017) Libing Yu et al.   eLife

Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA

(2017) Elizabeth A Hubin et al.   eLife

RNA Polymerase Pausing during Initial Transcription

(2016) Diego Duchi et al.   MOLECULAR CELL

Backtracked and paused transcription initiation intermediate ofEscherichia coliRNA polymerase

(2016) Eitan Lerner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

E. coli RNA Polymerase Determinants of Open Complex Lifetime and Structure

(2015) Emily F. Ruff et al.   JOURNAL OF MOLECULAR BIOLOGY

Crosslink Mapping at Amino Acid-Base Resolution Reveals the Path of Scrunched DNA in Initial Transcribing Complexes

(2015) Jared T. Winkelman et al.   MOLECULAR CELL

Crystal Structures of the E. coli Transcription Initiation Complexes with a Complete Bubble

(2015) Yuhong Zuo et al.   MOLECULAR CELL

Kinetics of promoter escape by bacterial RNA polymerase: effects of promoter contacts and transcription bubble collapse

(2014) Je Ko et al.   BIOCHEMICAL JOURNAL

Co-operative roles for DNA supercoiling and nucleoid-associated proteins in the regulation of bacterial transcription

(2013) Charles J. Dorman   BIOCHEMICAL SOCIETY TRANSACTIONS

Next Generation Sequencing-Based Parallel Analysis of Melting Kinetics of 4096 Variants of a Bacterial Promoter

(2013) Ewa Heyduk et al.   BIOCHEMISTRY

Key Roles of the Downstream Mobile Jaw of Escherichia coli RNA Polymerase in Transcription Initiation

(2012) Amanda Drennan et al.   BIOCHEMISTRY

Structural Basis of Transcription Initiation

(2012) Y. Zhang et al.   SCIENCE

Structural Basis for Promoter −10 Element Recognition by the Bacterial RNA Polymerase σ Subunit

(2011) Andrey Feklistov et al.   CELL

Mechanism of Bacterial Transcription Initiation: RNA Polymerase - Promoter Binding, Isomerization to Initiation-Competent Open Complexes, and Initiation of RNA Synthesis

(2011) Ruth M. Saecker et al.   JOURNAL OF MOLECULAR BIOLOGY

Probing DNA Binding, DNA Opening, and Assembly of a Downstream Clamp/Jaw inEscherichia coliRNA Polymerase−λPRPromoter Complexes Using Salt and the Physiological Anion Glutamate

(2010) Wayne S. Kontur et al.   BIOCHEMISTRY

One-step DNA melting in the RNA polymerase cleft opens the initiation bubble to form an unstable open complex

(2010) T. J. Gries et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thermal Probing of E. coli RNA Polymerase Off-Pathway Mechanisms

(2008) Yara X. Mejia et al.   JOURNAL OF MOLECULAR BIOLOGY

Fine structure of the promoter-  region 1.2 interaction

(2008) S. P. Haugen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Late Steps in the Formation of E. coli RNA Polymerase—λPR Promoter Open Complexes: Characterization of Conformational Changes by Rapid [Perturbant] Upshift Experiments

(2007) Wayne S. Kontur et al.   JOURNAL OF MOLECULAR BIOLOGY