Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 427, Issue 15, Pages 2435-2450Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2015.05.024
Keywords
transcription initiation; regulation; kinetics; mechanism; sigma(70) region 1.1
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Funding
- National Institutes of Health [GM103061, GM067153]
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In transcription initiation by Escherichia coli RNA polymerase (RNAP), initial binding to promoter DNA triggers large conformational changes, bending downstream duplex DNA into the RNAP cleft and opening 13 bp to form a short-lived open intermediate 02). Subsequent conformational changes increase lifetimes of lambda P-R and T7A1 open complexes (OCs) by >10(5)-fold and >10(2)-fold, respectively. OC lifetime is a target for regulation. To characterize late conformational changes, we determine effects on OC dissociation kinetics of deletions in RNAP mobile elements sigma(70) region 1.1 (sigma(1.1)), beta' jaw and beta' sequence insertion 3 (SI3). In very stable OC formed by the wild type WT RNAP with lambda P-R (RPO) and by Delta sigma(1.1) RNAP with lambda P-R or T7A1, we conclude that downstream duplex DNA is bound to the jaw in an assembly with SI3, and bases -4 to +2 of the nontemplate strand discriminator region are stably bound in a positively charged track in the cleft. We deduce that polyanionic sigma(1.1) destabilizes OC by competing for binding sites in the cleft and on the jaw with the polyanionic discriminator strand and downstream duplex, respectively. Examples of sigma(1.1)-destabilized OC are the final T7A1 OC and the lambda P-R I-3 intermediate OC. Deleting sigma(1.1) and either beta' jaw or SI3 equalizes OC lifetimes for lambda P-R and T7A1. DNA closing rates are similar for both promoters and all RNAP variants. We conclude that late conformational changes that stabilize OC, like early ones that bend the duplex into the cleft, are primary targets of regulation, while the intrinsic DNA opening/closing step is not. (C) 2015 Elsevier Ltd. All rights reserved.
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