CarD and RbpA modify the kinetics of initial transcription and slow promoter escape of the Mycobacterium tuberculosis RNA polymerase

Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding

(2019) Hande Boyaci et al.   NATURE

RNA Polymerase: Step-by-Step Kinetics and Mechanism of Transcription Initiation

(2019) Kate L. Henderson et al.   BIOCHEMISTRY

Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets

(2018) Jerome Prusa et al.   JOURNAL OF BACTERIOLOGY

DNA template sequence control of bacterial RNA polymerase escape from the promoter

(2018) Ewa Heyduk et al.   NUCLEIC ACIDS RESEARCH

Pausing controls branching between productive and non-productive pathways during initial transcription in bacteria

(2018) David Dulin et al.   Nature Communications

Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts

(2018) Hande Boyaci et al.   eLife

The calculation of transcript flux ratios reveals single regulatory mechanisms capable of activation and repression

(2018) Eric A. Galburt   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanism of transcription initiation and promoter escape byE.coliRNA polymerase

(2017) Kate L. Henderson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural insights into the mycobacteria transcription initiation complex from analysis of X-ray crystal structures

(2017) Elizabeth A. Hubin et al.   Nature Communications

Structure and function of the mycobacterial transcription initiation complex with the essential regulator RbpA

(2017) Elizabeth A Hubin et al.   eLife

Real-Time Observation of Backtracking by Bacterial RNA Polymerase

(2016) Agnieszka Lass-Napiorkowska et al.   BIOCHEMISTRY

Bacterial Transcription as a Target for Antibacterial Drug Development

(2016) Cong Ma et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

RNA Polymerase Pausing during Initial Transcription

(2016) Diego Duchi et al.   MOLECULAR CELL

Local and global regulation of transcription initiation in bacteria

(2016) Douglas F. Browning et al.   NATURE REVIEWS MICROBIOLOGY

Backtracked and paused transcription initiation intermediate ofEscherichia coliRNA polymerase

(2016) Eitan Lerner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CarD stabilizes mycobacterial open complexes via a two-tiered kinetic mechanism

(2015) Jayan Rammohan et al.   NUCLEIC ACIDS RESEARCH

Structural, functional, and genetic analyses of the actinobacterial transcription factor RbpA

(2015) Elizabeth A. Hubin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex

(2015) Brian Bae et al.   eLife

Bacterial Sigma Factors: A Historical, Structural, and Genomic Perspective

(2014) Andrey Feklístov et al.   Annual Review of Microbiology

Kinetics of promoter escape by bacterial RNA polymerase: effects of promoter contacts and transcription bubble collapse

(2014) Je Ko et al.   BIOCHEMICAL JOURNAL

CarD integrates three functional modules to promote efficient transcription, antibiotic tolerance, and pathogenesis in mycobacteria

(2014) Ashley L. Garner et al.   MOLECULAR MICROBIOLOGY

Distinct functions of the RNA polymerase σ subunit region 3.2 in RNA priming and promoter escape

(2014) Danil Pupov et al.   NUCLEIC ACIDS RESEARCH

Mycobacterial RNA polymerase forms unstable open promoter complexes that are stabilized by CarD

(2014) Elizabeth Davis et al.   NUCLEIC ACIDS RESEARCH

Optimization of recombinant Mycobacterium tuberculosis RNA polymerase expression and purification

(2014) Rajdeep Banerjee et al.   TUBERCULOSIS

Mycobacterium tuberculosisRNA Polymerase-binding Protein A (RbpA) and Its Interactions with Sigma Factors

(2013) Alessio Bortoluzzi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and function of CarD, an essential mycobacterial transcription factor

(2013) D. B. Srivastava et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Mtb CarD/RNAP β-Lobes Complex Reveals the Molecular Basis of Interaction and Presents a Distinct DNA-Binding Domain for Mtb CarD

(2013) Gulcin Gulten et al.   STRUCTURE

Interaction of CarD with RNA Polymerase Mediates Mycobacterium tuberculosis Viability, Rifampin Resistance, and Pathogenesis

(2012) L. A. Weiss et al.   JOURNAL OF BACTERIOLOGY

Mycobacterium tuberculosis RbpA protein is a new type of transcriptional activator that stabilizes the σ A -containing RNA polymerase holoenzyme

(2012) Yangbo Hu et al.   NUCLEIC ACIDS RESEARCH

Distinct and Contrasting Transcription Initiation Patterns at Mycobacterium tuberculosis Promoters

(2012) Priyanka Tare et al.   PLoS One

Mechanism of Bacterial Transcription Initiation: RNA Polymerase - Promoter Binding, Isomerization to Initiation-Competent Open Complexes, and Initiation of RNA Synthesis

(2011) Ruth M. Saecker et al.   JOURNAL OF MOLECULAR BIOLOGY

A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival

(2011) Arnab China et al.   PLoS One

Isolation of conditional expression mutants in Mycobacterium tuberculosis by transposon mutagenesis

(2011) Francesca Forti et al.   TUBERCULOSIS

Comparison of promoter-specific events during transcription initiation in mycobacteria

(2010) A. China et al.   MICROBIOLOGY-SGM

Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction

(2010) Lars F. Westblade et al.   NUCLEIC ACIDS RESEARCH

CarD Is an Essential Regulator of rRNA Transcription Required for Mycobacterium tuberculosis Persistence

(2009) Christina L. Stallings et al.   CELL

Global Kinetic Explorer: A new computer program for dynamic simulation and fitting of kinetic data

(2008) Kenneth A. Johnson et al.   ANALYTICAL BIOCHEMISTRY

Analysis of RNA polymerase-promoter complex formation

(2008) Wilma Ross et al.   METHODS

RNA polymerase: the vehicle of transcription

(2008) Sergei Borukhov et al.   TRENDS IN MICROBIOLOGY

The Generation of Promoter-Mediated Transcriptional Noise in Bacteria

(2008) Namiko Mitarai et al.   PLoS Computational Biology