O-Linked-N-Acetylglucosaminylation of the RNA-Binding Protein EWS N-Terminal Low Complexity Region Reduces Phase Separation and Enhances Condensate Dynamics

Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase

(2021) Zebulon G. Levine et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The human O-GlcNAcome database and meta-analysis

(2021) Eugenia Wulff-Fuentes et al.   Scientific Data

O-GlcNAc modification of small heat shock proteins enhances their anti-amyloid chaperone activity

(2021) Aaron T. Balana et al.   Nature Chemistry

Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase

(2021) Tae Hun Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TDP-43 α-helical structure tunes liquid–liquid phase separation and function

(2020) Alexander E. Conicella et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Poly(ADP-ribose): A Dynamic Trigger for Biomolecular Condensate Formation

(2020) Anthony K.L. Leung   TRENDS IN CELL BIOLOGY

α-Synuclein aggregation nucleates through liquid–liquid phase separation

(2020) Soumik Ray et al.   Nature Chemistry

Liquid-liquid phase separation induces pathogenic tau conformations in vitro

(2020) Nicholas M. Kanaan et al.   Nature Communications

Tandem Bioorthogonal Labeling Uncovers Endogenous Cotranslationally O-GlcNAc Modified Nascent Proteins

(2020) Yanping Zhu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

RNA-binding and prion domains: the Yin and Yang of phase separation

(2020) Nieves Lorenzo Gotor et al.   NUCLEIC ACIDS RESEARCH

Phase Separation as a Missing Mechanism for Interpretation of Disease Mutations

(2020) Brian Tsang et al.   CELL

Regulatory mechanisms of tau protein fibrillation under the conditions of liquid–liquid phase separation

(2020) Solomiia Boyko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nutrient Regulation of Signaling & Transcription

(2019) Gerald W. Hart   JOURNAL OF BIOLOGICAL CHEMISTRY

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The synergic effect of water and biomolecules in intracellular phase separation

(2019) Sara S. Ribeiro et al.   Nature Reviews Chemistry

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

(2019) Yang Eric Guo et al.   NATURE

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching

(2019) Nicole O. Taylor et al.   BIOPHYSICAL JOURNAL

First-generation predictors of biological protein phase separation

(2019) Robert M Vernon et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Role of Post-Translational Modifications in the Phase Transitions of Intrinsically Disordered Proteins

(2019) Izzy Owen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

TATA-Box Binding Protein O-GlcNAcylation at T114 Regulates Formation of the B-TFIID Complex and Is Critical for Metabolic Gene Regulation

(2019) Stéphan Hardivillé et al.   MOLECULAR CELL

Theories for Sequence-Dependent Phase Behaviors of Biomolecular Condensates

(2018) Yi-Hsuan Lin et al.   BIOCHEMISTRY

Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation

(2018) Mario Hofweber et al.   CELL

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Nuclear-Import Receptors Reverse Aberrant Phase Transitions of RNA-Binding Proteins with Prion-like Domains

(2018) Lin Guo et al.   CELL

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing

(2018) Ailin Wang et al.   EMBO JOURNAL

Tau protein liquid–liquid phase separation can initiate tau aggregation

(2018) Susanne Wegmann et al.   EMBO JOURNAL

Prion-like low-complexity sequences: Key regulators of protein solubility and phase behavior

(2018) Titus Franzmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior

(2018) Tarique Khan et al.   MOLECULAR CELL

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Phase separation of a yeast prion protein promotes cellular fitness

(2018) Titus M. Franzmann et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery

(2018) Sonja Kroschwald et al.   Cell Reports

Pi-Pi contacts are an overlooked protein feature relevant to phase separation

(2018) Robert McCoy Vernon et al.   eLife

O-GlcNAc: A Sweetheart of the Cell Cycle and DNA Damage Response

(2018) Caifei Liu et al.   Frontiers in Endocrinology

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Friend or foe — post-translational modifications as regulators of phase separation and RNP granule dynamics

(2018) Mario Hofweber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural characterization of the D290V mutation site in hnRNPA2 low-complexity–domain polymers

(2018) Dylan T. Murray et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

O-GlcNAc as an Integrator of Signaling Pathways

(2018) Qunxiang Ong et al.   Frontiers in Endocrinology

Nutrient-driven O -GlcNAc in proteostasis and neurodegeneration

(2017) Ilhan Akan et al.   JOURNAL OF NEUROCHEMISTRY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Protein O-GlcNAcylation: emerging mechanisms and functions

(2017) Xiaoyong Yang et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation

(2017) Tariq Afroz et al.   Nature Communications

A Conserved Splicing Silencer Dynamically Regulates O-GlcNAc Transferase Intron Retention and O-GlcNAc Homeostasis

(2017) Sung-Kyun Park et al.   Cell Reports

The glycosylation stoichiometry of EWS species in neuronal cells

(2016) Kazuo Kamemura et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

O-GlcNAc glycosylation stoichiometry of the FET protein family: only EWS is glycosylated with a high stoichiometry

(2016) Kazuo Kamemura   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Nutrient regulation of gene expression by O-GlcNAcylation of chromatin

(2016) Stéphan Hardivillé et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A Concentration-Dependent Liquid Phase Separation Can Cause Toxicity upon Increased Protein Expression

(2016) Benedetta Bolognesi et al.   Cell Reports

A Small Molecule That Inhibits OGT Activity in Cells

(2015) Rodrigo F. Ortiz-Meoz et al.   ACS Chemical Biology

Biochemical Properties and Biological Functions of FET Proteins

(2015) Jacob C. Schwartz et al.   Annual Review of Biochemistry

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles

(2015) Sven Hennig et al.   JOURNAL OF CELL BIOLOGY

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

O-GlcNAc modification blocks the aggregation and toxicity of the protein α-synuclein associated with Parkinson's disease

(2015) Nicholas P. Marotta et al.   Nature Chemistry

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

PLAAC: a web and command-line application to identify proteins with prion-like amino acid composition

(2014) A. K. Lancaster et al.   BIOINFORMATICS

O-GlcNAcylation Prevents Aggregation of the Polycomb Group Repressor Polyhomeotic

(2014) Maria Cristina Gambetta et al.   DEVELOPMENTAL CELL

Assemblages: Functional units formed by cellular phase separation

(2014) Jeffrey A. Toretsky et al.   JOURNAL OF CELL BIOLOGY

O-GlcNAc Modification of tau Directly Inhibits Its Aggregation without Perturbing the Conformational Properties of tau Monomers

(2014) Scott A. Yuzwa et al.   JOURNAL OF MOLECULAR BIOLOGY

O-GlcNAc Transferase Integrates Metabolic Pathways to Regulate the Stability of c-MYC in Human Prostate Cancer Cells

(2013) H. M. Itkonen et al.   CANCER RESEARCH

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis

(2013) Jennifer A. Groves et al.   CELL STRESS & CHAPERONES

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes

(2012) Aksana A Labokha et al.   EMBO JOURNAL

Alternative 3′-end processing of long noncoding RNA initiates construction of nuclear paraspeckles

(2012) Takao Naganuma et al.   EMBO JOURNAL

Evidence of the Involvement ofO-GlcNAc-modified Human RNA Polymerase II CTD in Transcriptionin Vitroandin Vivo

(2012) Stella M. Ranuncolo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Increasing O-GlcNAc slows neurodegeneration and stabilizes tau against aggregation

(2012) Scott A Yuzwa et al.   Nature Chemical Biology

Cross Talk Between O-GlcNAcylation and Phosphorylation: Roles in Signaling, Transcription, and Chronic Disease

(2011) Gerald W. Hart et al.   Annual Review of Biochemistry

Hijacking a biosynthetic pathway yields a glycosyltransferase inhibitor within cells

(2011) Tracey M Gloster et al.   Nature Chemical Biology

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

O-GlcNAcylation Determines the Solubility, Filament Organization, and Stability of Keratins 8 and 18

(2010) Budnar Srikanth et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

O-GlcNAcylation is involved in the transcriptional activity of EWS-FLI1 in Ewing's sarcoma

(2009) R Bachmaier et al.   ONCOGENE

A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly

(2008) Takbum Ohn et al.   NATURE CELL BIOLOGY