Targeted CHO cell engineering approaches can reduce HCP‐related enzymatic degradation and improve mAb product quality

A Novel Hydrogen Peroxide Evolved CHO Host can Improve the Expression of Difficult to Express Bispecific Antibodies.

(2021) Rajesh K Mistry et al.   BIOTECHNOLOGY AND BIOENGINEERING

Multiplex secretome engineering enhances recombinant protein production and purity

(2020) Stefan Kol et al.   Nature Communications

CHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant

(2019) Cher H. Goey et al.   BIOCHEMICAL ENGINEERING JOURNAL

Identification and CRISPR/Cas9 Knockout of the Endogenous C1s Protease in CHO Cells Eliminates Aberrant Proteolysis of Recombinantly Expressed Proteins

(2019) Sophia Li et al.   BIOTECHNOLOGY AND BIOENGINEERING

Anxa2 ‐ and Ctsd ‐knockout CHO cell lines to diminish the risk of contamination with host cell proteins

(2019) Nobuo Fukuda et al.   BIOTECHNOLOGY PROGRESS

Identification and characterization of co-purifying CHO host cell proteins in monoclonal antibody purification process

(2019) Xinrong Liu et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production

(2019) Tingting Cui et al.   JOURNAL OF BIOTECHNOLOGY

Host cell protein removal from biopharmaceutical preparations: Towards the implementation of quality by design

(2018) Cher Hui Goey et al.   BIOTECHNOLOGY ADVANCES

Degradation of recombinant proteins by Chinese hamster ovary host cell proteases is prevented by matriptase-1 knockout

(2018) Holger Laux et al.   BIOTECHNOLOGY AND BIOENGINEERING

A reference genome of the Chinese hamster based on a hybrid assembly strategy

(2018) Oliver Rupp et al.   BIOTECHNOLOGY AND BIOENGINEERING

RNA-Seq Highlights High Clonal Variation in Monoclonal Antibody Producing CHO Cells

(2018) Camila A. Orellana et al.   Biotechnology Journal

Experience with host cell protein impurities in biopharmaceuticals

(2018) Martin Vanderlaan et al.   BIOTECHNOLOGY PROGRESS

Characterization of Phenotypic and Genotypic Diversity in Subclones derived from a Clonal Cell Line

(2018) Tharmala Tharmalingam et al.   BIOTECHNOLOGY PROGRESS

RNA-Seq Highlights High Clonal Variation in Monoclonal Antibody Producing CHO Cells

(2018) Camila A. Orellana et al.   Biotechnology Journal

Transcriptome-Based Identification of the Optimal Reference CHO Genes for Normalisation of qPCR Data

(2017) Adam J. Brown et al.   Biotechnology Journal

Lysosomal adaptation: How cells respond to lysosomotropic compounds

(2017) Shuyan Lu et al.   PLoS One

Specific Immune Response to Phospholipase B-Like 2 Protein, a Host Cell Impurity in Lebrikizumab Clinical Material

(2016) Saloumeh Kadkhodayan Fischer et al.   AAPS Journal

Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations

(2016) Josephine Chiu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Characterization of the co-elution of host cell proteins with monoclonal antibodies during protein A purification

(2016) Qingchun Zhang et al.   BIOTECHNOLOGY PROGRESS

Identification of an IgG CDR sequence contributing to co-purification of the host cell protease cathepsin D

(2016) Jared S. Bee et al.   BIOTECHNOLOGY PROGRESS

Expression of difficult-to-remove host cell protein impurities during extended Chinese hamster ovary cell culture and their impact on continuous bioprocessing

(2015) Kristin N. Valente et al.   BIOTECHNOLOGY AND BIOENGINEERING

Trace levels of the CHO host cell protease cathepsin D caused particle formation in a monoclonal antibody product

(2015) Jared S. Bee et al.   BIOTECHNOLOGY PROGRESS

Mammalian cell-produced therapeutic proteins: heterogeneity derived from protein degradation

(2014) Haimanti Dorai et al.   CURRENT OPINION IN BIOTECHNOLOGY

Improved detection of host cell proteins (HCPs) in a mammalian cell-derived antibody drug using liquid chromatography/mass spectrometry in conjunction with an HCP-enrichment strategy

(2014) Jenny Heidbrink Thompson et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing

(2013) Nicholas E. Levy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Functional heterogeneity and heritability in CHO cell populations

(2012) Sarah L. Davies et al.   BIOTECHNOLOGY AND BIOENGINEERING

Degradation of an Fc-fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

(2009) Flavie Robert et al.   BIOTECHNOLOGY AND BIOENGINEERING

Host cell proteins in biologics development: Identification, quantitation and risk assessment

(2009) Xing Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Gene Network Regulating Lysosomal Biogenesis and Function

(2009) Marco Sardiello et al.   SCIENCE