One-Pot Conversion of Free Sialoglycans to Functionalized Glycan Oxazolines and Efficient Synthesis of Homogeneous Antibody–Drug Conjugates through Site-Specific Chemoenzymatic Glycan Remodeling

Unlocking the potential of antibody–drug conjugates for cancer therapy

(2021) Joshua Z. Drago et al.   Nature Reviews Clinical Oncology

Antibody Glycoengineering and Homogeneous Antibody‐Drug Conjugate Preparation

(2021) Shino Manabe et al.   CHEMICAL RECORD

The oncology market for antibody–drug conjugates

(2021) Carolina do Pazo et al.   NATURE REVIEWS DRUG DISCOVERY

Recent progress in transglutaminase-mediated assembly of antibody-drug conjugates

(2020) Hendrik Schneider et al.   ANALYTICAL BIOCHEMISTRY

Glycoengineering of Antibodies for Modulating Functions

(2019) Lai-Xi Wang et al.   Annual Review of Biochemistry

Development of α-Gal-Antibody Conjugates to Increase Immune Response by Recruiting Natural Antibodies

(2019) Julinton Sianturi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Characterization of Antibody Products Obtained through Enzymatic and Nonenzymatic Glycosylation Reactions with a Glycan Oxazoline and Preparation of a Homogeneous Antibody–Drug Conjugate via Fc N-Glycan

(2019) Shino Manabe et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Bioconjugation and Multi-Bioorthogonal Labeling via Rapid Formation of a Boron–Nitrogen Heterocycle

(2019) Tak Ian Chio et al.   BIOCONJUGATE CHEMISTRY

Substrate Design Enables Heterobifunctional, Dual “Click” Antibody Modification via Microbial Transglutaminase

(2019) Joshua A. Walker et al.   BIOCONJUGATE CHEMISTRY

Rapid and Efficient Generation of Stable Antibody-Drug Conjugates via an Encoded Cyclopropene and an Inverse-Electron-Demand Diels-Alder Reaction

(2018) Benjamí Oller-Salvia et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Selective Cysteine-Cyclooctyne Conjugation

(2018) Chi Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-specific immobilization of endoglycosidases for streamlined chemoenzymatic glycan remodeling of antibodies

(2018) Tiezheng Li et al.   CARBOHYDRATE RESEARCH

Amide Bond Formation of Sialic Acid in Oligosaccharide without Protecting Group

(2018) Shino Manabe et al.   HETEROCYCLES

Generation of efficient mutants of endoglycosidase from Streptococcus pyogenes and their application in a novel one-pot transglycosylation reaction for antibody modification

(2018) Mitsuhiro Iwamoto et al.   PLoS One

Chemoenzymatic Methods for the Synthesis of Glycoproteins

(2018) Chao Li et al.   CHEMICAL REVIEWS

Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody–drug conjugates

(2017) Feng Tang et al.   Nature Protocols

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Enzymatic conjugation using branched linkers for constructing homogeneous antibody–drug conjugates with high potency

(2017) Yasuaki Anami et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Modulating IgG effector function by Fc glycan engineering

(2017) Tiezheng Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Redox-based reagents for chemoselective methionine bioconjugation

(2017) Shixian Lin et al.   SCIENCE

Optimal Synthetic Glycosylation of a Therapeutic Antibody

(2016) Thomas B. Parsons et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

One-Step Conjugation Method for Site-Specific Antibody–Drug Conjugates through Reactive Cysteine-Engineered Antibodies

(2016) Daisuke Shinmi et al.   BIOCONJUGATE CHEMISTRY

Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling

(2016) Tiezheng Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

One-pot N-glycosylation remodeling of IgG with non-natural sialylglycopeptides enables glycosite-specific and dual-payload antibody–drug conjugates

(2016) Feng Tang et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Chemoenzymatic Conjugation of Toxic Payloads to the Globally Conserved N-Glycan of Native mAbs Provides Homogeneous and Highly Efficacious Antibody–Drug Conjugates

(2015) Remon van Geel et al.   BIOCONJUGATE CHEMISTRY

Genetically Encoded Azide Containing Amino Acid in Mammalian Cells Enables Site-Specific Antibody–Drug Conjugates Using Click Cycloaddition Chemistry

(2015) Michael P. VanBrunt et al.   BIOCONJUGATE CHEMISTRY

Tumor Cells Chronically Treated with a Trastuzumab-Maytansinoid Antibody-Drug Conjugate Develop Varied Resistance Mechanisms but Respond to Alternate Treatments

(2015) F. Loganzo et al.   MOLECULAR CANCER THERAPEUTICS

Site-specific conjugation improves therapeutic index of antibody drug conjugates with high drug loading

(2015) Pavel Strop et al.   NATURE BIOTECHNOLOGY

Antibody-Drug Conjugates: An Emerging Concept in Cancer Therapy

(2014) Ravi V. J. Chari et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Preparation of Well-Defined Antibody-Drug Conjugates through Glycan Remodeling and Strain-Promoted Azide-Alkyne Cycloadditions

(2014) Xiuru Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bridging Disulfides for Stable and Defined Antibody Drug Conjugates

(2014) George Badescu et al.   BIOCONJUGATE CHEMISTRY

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Site-Specific Antibody–Drug Conjugation through Glycoengineering

(2014) Qun Zhou et al.   BIOCONJUGATE CHEMISTRY

Site-specific antibody-drug conjugation through an engineered glycotransferase and a chemically reactive sugar

(2014) Zhongyu Zhu et al.   mAbs

Metabolic Engineering of Monoclonal Antibody Carbohydrates for Antibody–Drug Conjugation

(2013) Nicole M. Okeley et al.   BIOCONJUGATE CHEMISTRY

A dimethoxytriazine type glycosyl donor enables a facile chemo-enzymatic route toward α-linked N-acetylglucosaminyl-galactose disaccharide unit from gastric mucin

(2012) Masato Noguchi et al.   CHEMICAL COMMUNICATIONS

Site-Specific Traceless Coupling of Potent Cytotoxic Drugs to Recombinant Antibodies for Pharmacodelivery

(2012) Giulio Casi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoenzymatic Glycoengineering of Intact IgG Antibodies for Gain of Functions

(2012) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates

(2012) Ben-Quan Shen et al.   NATURE BIOTECHNOLOGY

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient Synthesis of Sugar Oxazolines from UnprotectedN-Acetyl-2-amino Sugars by Using Chloroformamidinium Reagent in Water

(2009) Masato Noguchi et al.   JOURNAL OF ORGANIC CHEMISTRY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY