Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase

Engineering the Substrate Specificity of a Modular Polyketide Synthase for Installation of Consecutive Non-Natural Extender Units

(2019) Edward Kalkreuter et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The hidden enzymology of bacterial natural product biosynthesis

(2019) Thomas A. Scott et al.   Nature Reviews Chemistry

Structural Basis of a Broadly Selective Acyltransferase from the Polyketide Synthase of Splenocin

(2018) Yuan Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering enzymatic assembly lines for the production of new antimicrobials

(2018) Edward Kalkreuter et al.   CURRENT OPINION IN MICROBIOLOGY

Flexible enzymatic activation of artificial polyketide extender units by Streptomyces cinnamonensis into the monensin biosynthetic pathway

(2018) D. Möller et al.   LETTERS IN APPLIED MICROBIOLOGY

Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups

(2018) Jie-Jie Shen et al.   Frontiers in Microbiology

Extender Unit Promiscuity and Orthogonal Protein Interactions of an Aminomalonyl-ACP Utilizing Trans-Acyltransferase from Zwittermicin Biosynthesis

(2018) Samantha M. Carpenter et al.   ACS Chemical Biology

Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

(2017) Ewa M. Musiol-Kroll et al.   ACS Synthetic Biology

Inversion of Extender Unit Selectivity in the Erythromycin Polyketide Synthase by Acyltransferase Domain Engineering

(2016) Irina Koryakina et al.   ACS Chemical Biology

Natural product discovery: past, present, and future

(2016) Leonard Katz et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units

(2016) Anja Greule et al.   MOLECULES

Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production

(2016) Satoshi Yuzawa et al.   ACS Synthetic Biology

Substrate Flexibility of a Mutated Acyltransferase Domain and Implications for Polyketide Biosynthesis

(2015) Kenny Bravo-Rodriguez et al.   CHEMISTRY & BIOLOGY

ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB

(2015) James A. Maier et al.   Journal of Chemical Theory and Computation

New ways to boost molecular dynamics simulations

(2015) Elmar Krieger et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Evolution of Efficient Modular Polyketide Synthases by Homologous Recombination

(2015) Joseph A. Chemler et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

YASARA View—molecular graphics for all devices—from smartphones to workstations

(2014) Elmar Krieger et al.   BIOINFORMATICS

Predicted Incorporation of Non-native Substrates by a Polyketide Synthase Yields Bioactive Natural Product Derivatives

(2014) Kenny Bravo-Rodriguez et al.   CHEMBIOCHEM

Promiscuity of a modular polyketide synthase towards natural and non-natural extender units

(2013) Irina Koryakina et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Classification of the Adenylation and Acyl-Transferase Activity of NRPS and PKS Systems Using Ensembles of Substrate Specific Hidden Markov Models

(2013) Barzan I. Khayatt et al.   PLoS One

Expanding the Fluorine Chemistry of Living Systems Using Engineered Polyketide Synthase Pathways

(2013) M. C. Walker et al.   SCIENCE

Poly Specific trans-Acyltransferase Machinery Revealed via Engineered Acyl-CoA Synthetases

(2012) Irina Koryakina et al.   ACS Chemical Biology

Enzyme-Directed Mutasynthesis: A Combined Experimental and Theoretical Approach to Substrate Recognition of a Polyketide Synthase

(2012) Uschi Sundermann et al.   ACS Chemical Biology

Mining the Cinnabaramide Biosynthetic Pathway to Generate Novel Proteasome Inhibitors

(2011) Shwan Rachid et al.   CHEMBIOCHEM

I-TASSER: a unified platform for automated protein structure and function prediction

(2010) Ambrish Roy et al.   Nature Protocols

Structures and Mechanisms of Polyketide Synthases

(2009) Chaitan Khosla   JOURNAL OF ORGANIC CHEMISTRY

Synthesis and Biochemical Analysis of Complex Chain-Elongation Intermediates for Interrogation of Molecular Specificity in the Erythromycin and Pikromycin Polyketide Synthases

(2009) Jonathan D. Mortison et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

I-TASSER server for protein 3D structure prediction

(2008) Yang Zhang   BMC BIOINFORMATICS

Biosynthesis of polyketide synthase extender units

(2008) Yolande A. Chan et al.   NATURAL PRODUCT REPORTS

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

(2007) Hrvoje Petković et al.   ORGANIC & BIOMOLECULAR CHEMISTRY