Phase Separation in Genome Organization across Evolution

Chromatin mechanics dictates subdiffusion and coarsening dynamics of embedded condensates

(2021) Daniel S. W. Lee et al.   Nature Physics

Biomolecular condensation of NUP98 fusion proteins drives leukemogenic gene expression

(2021) Stefan Terlecki-Zaniewicz et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Self‐assembly of multi‐component mitochondrial nucleoids via phase separation

(2021) Marina Feric et al.   EMBO JOURNAL

HP1 proteins compact DNA into mechanically and positionally stable phase separated domains

(2021) Madeline M Keenen et al.   eLife

Bridging-induced phase separation induced by cohesin SMC protein complexes

(2021) Je-Kyung Ryu et al.   Science Advances

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Phase separation of Epstein-Barr Virus EBNA2 and Its Coactivator EBNALP controls gene expression

(2020) Qiu Peng et al.   JOURNAL OF VIROLOGY

Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation

(2020) Fabian Erdel et al.   MOLECULAR CELL

Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria

(2020) Abul K. Tarafder et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

What Is a Transcriptional Burst?

(2020) Edward Tunnacliffe et al.   TRENDS IN GENETICS

Biomolecular Phase Separation: From Molecular Driving Forces to Macroscopic Properties

(2020) Gregory L. Dignon et al.   Annual Review of Physical Chemistry

Making the Case for Disordered Proteins and Biomolecular Condensates in Bacteria

(2020) Megan C. Cohan et al.   TRENDS IN BIOCHEMICAL SCIENCES

Architecture of the herpesvirus genome-packaging complex and implications for DNA translocation

(2020) Yunxiang Yang et al.   Protein & Cell

Liquid-like interactions in heterochromatin: Implications for mechanism and regulation

(2020) Serena Sanulli et al.   CURRENT OPINION IN CELL BIOLOGY

CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies

(2020) Woonyung Hur et al.   DEVELOPMENTAL CELL

Nuclear body phase separation drives telomere clustering in ALT cancer cells

(2020) Huaiying Zhang et al.   MOLECULAR BIOLOGY OF THE CELL

Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

(2020) Anne-Marie Ladouceur et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Partitioning of cancer therapeutics in nuclear condensates

(2020) Isaac A. Klein et al.   SCIENCE

Biomolecular Condensates in the Nucleus

(2020) Benjamin R. Sabari et al.   TRENDS IN BIOCHEMICAL SCIENCES

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling

(2020) Soumya G. Remesh et al.   Nature Communications

The Self-Organizing Genome: Principles of Genome Architecture and Function

(2020) Tom Misteli   CELL

Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly

(2020) Sara Cuylen-Haering et al.   NATURE

A protein assembly mediates Xist localization and gene silencing

(2020) Amy Pandya-Jones et al.   NATURE

Nucleated transcriptional condensates amplify gene expression

(2020) Ming-Tzo Wei et al.   NATURE CELL BIOLOGY

Phase separation by ssDNA binding protein controlled via protein−protein and protein−DNA interactions

(2020) Gábor M. Harami et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chromatin arranges in chains of mesoscale domains with nanoscale functional topography independent of cohesin

(2020) Ezequiel Miron et al.   Science Advances

Condensed Chromatin Behaves like a Solid on the Mesoscale In Vitro and in Living Cells

(2020) Hilmar Strickfaden et al.   CELL

RNA-Mediated Feedback Control of Transcriptional Condensates

(2020) Jonathan E. Henninger et al.   CELL

Crystal engineering with DNA

(2019) Christine R. Laramy et al.   Nature Reviews Materials

The C-Terminal Domain of RNA Polymerase II Is a Multivalent Targeting Sequence that Supports Drosophila Development with Only Consensus Heptads

(2019) Feiyue Lu et al.   MOLECULAR CELL

Extensive Heterogeneity and Intrinsic Variation in Spatial Genome Organization

(2019) Elizabeth H. Finn et al.   CELL

3D genome organization: a role for phase separation and loop extrusion?

(2019) Maike Stam et al.   CURRENT OPINION IN PLANT BIOLOGY

Cryo-EM structures of herpes simplex virus type 1 portal vertex and packaged genome

(2019) Yun-Tao Liu et al.   NATURE

Phase separation drives X-chromosome inactivation: a hypothesis

(2019) Andrea Cerase et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

Implications of liquid–liquid phase separation in plant chromatin organization and transcriptional control

(2019) Nan Wang et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Phase separation of 53 BP 1 determines liquid‐like behavior of DNA repair compartments

(2019) Sinan Kilic et al.   EMBO JOURNAL

Heterochromatin drives compartmentalization of inverted and conventional nuclei

(2019) Martin Falk et al.   NATURE

Microstructure arrays of DNA using topographic control

(2019) Yun Jeong Cha et al.   Nature Communications

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

Histone Modifications Regulate Chromatin Compartmentalization by Contributing to a Phase Separation Mechanism

(2019) Liang Wang et al.   MOLECULAR CELL

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

(2019) Yang Eric Guo et al.   NATURE

The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex

(2019) Prasad Trivedi et al.   NATURE CELL BIOLOGY

Molecular basis and biological function of variability in spatial genome organization

(2019) Elizabeth H. Finn et al.   SCIENCE

Phase Separation as a Melting Pot for DNA Repeats

(2019) Amanda C. Hall et al.   TRENDS IN GENETICS

A new class of disordered elements controls DNA replication through initiator self-assembly

(2019) Matthew W Parker et al.   eLife

Organization of Chromatin by Intrinsic and Regulated Phase Separation

(2019) Bryan A. Gibson et al.   CELL

HP1 reshapes nucleosome core to promote phase separation of heterochromatin

(2019) S. Sanulli et al.   NATURE

Chromosome organization in bacteria: mechanistic insights into genome structure and function

(2019) Remus T. Dame et al.   NATURE REVIEWS GENETICS

Molecular structure in biomolecular condensates

(2019) Ivan Peran et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Phase separation of YAP reorganizes genome topology for long-term YAP target gene expression

(2019) Danfeng Cai et al.   NATURE CELL BIOLOGY

Chromatin domains in space and their functional implications

(2019) Frédéric Pontvianne et al.   CURRENT OPINION IN PLANT BIOLOGY

Formation of Chromatin Subcompartments by Phase Separation

(2018) Fabian Erdel et al.   BIOPHYSICAL JOURNAL

Chromatin organization by an interplay of loop extrusion and compartmental segregation

(2018) Johannes Nuebler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

Global DNA Compaction in Stationary-Phase Bacteria Does Not Affect Transcription

(2018) Richard Janissen et al.   CELL

Transcription Elongation Can Affect Genome 3D Structure

(2018) Sven Heinz et al.   CELL

Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments

(2018) Jill J. Bouchard et al.   MOLECULAR CELL

Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II

(2018) Huasong Lu et al.   NATURE

Kinase-controlled phase transition of membraneless organelles in mitosis

(2018) Arpan Kumar Rai et al.   NATURE

RNA polymerase II clustering through carboxy-terminal domain phase separation

(2018) Marc Boehning et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A segregative phase separation scenario of the formation of the bacterial nucleoid

(2018) Marc Joyeux   Soft Matter

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome

(2018) Yongdae Shin et al.   CELL

Plant HP1 protein ADCP1 links multivalent H3K9 methylation readout to heterochromatin formation

(2018) Shuai Zhao et al.   CELL RESEARCH

Nuclear condensates of the Polycomb protein chromobox 2 (CBX2) assemble through phase separation

(2018) Roubina Tatavosian et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Highly disordered histone H1−DNA model complexes and their condensates

(2018) Abigail L. Turner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging

(2017) Tadasu Nozaki et al.   MOLECULAR CELL

Phase separation drives heterochromatin domain formation

(2017) Amy R. Strom et al.   NATURE

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin

(2017) Adam G. Larson et al.   NATURE

Molecular engineering of chiral colloidal liquid crystals using DNA origami

(2017) Mahsa Siavashpouri et al.   NATURE MATERIALS

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Potential Role of Phase Separation of Repetitive DNA in Chromosomal Organization

(2017) Shao-Jun Tang   Genes

Mechanisms of ATP-Dependent Chromatin Remodeling Motors

(2016) Coral Y. Zhou et al.   Annual Review of Biophysics

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Structure of transcribing mammalian RNA polymerase II

(2016) Carrie Bernecky et al.   NATURE

Ki-67 acts as a biological surfactant to disperse mitotic chromosomes

(2016) Sara Cuylen et al.   NATURE

The molecular hallmarks of epigenetic control

(2016) C. David Allis et al.   NATURE REVIEWS GENETICS

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation

(2015) Avinash Patel et al.   CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion

(2015) Jens B. Bosse et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Live-cell superresolution microscopy reveals the organization of RNA polymerase in the bacterial nucleoid

(2015) Mathew Stracy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Evolutionary diversity and taxon-specific modifications of the RNA polymerase II C-terminal domain

(2014) C. Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

Organization and segregation of bacterial chromosomes

(2013) Xindan Wang et al.   NATURE REVIEWS GENETICS

Aqueous Phase Separation as a Possible Route to Compartmentalization of Biological Molecules

(2012) Christine D. Keating   ACCOUNTS OF CHEMICAL RESEARCH

Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter

(2011) Anna Rubio-Cosials et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The mitochondrial transcription and packaging factor Tfam imposes a U-turn on mitochondrial DNA

(2011) Huu B Ngo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mechanisms of ATP-dependent nucleosome sliding

(2010) Gregory D Bowman   CURRENT OPINION IN STRUCTURAL BIOLOGY

Bacterial nucleoid-associated proteins, nucleoid structure and gene expression

(2010) Shane C. Dillon et al.   NATURE REVIEWS MICROBIOLOGY

Biophysical characterizations of human mitochondrial transcription factor A and its binding to tumor suppressor p53

(2009) Tuck Seng Wong et al.   NUCLEIC ACIDS RESEARCH

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

The X-ray crystal structure of RNA polymerase from Archaea

(2008) Akira Hirata et al.   NATURE