Adenosine triphosphate energy‐independently controls protein homeostasis with unique structure and diverse mechanisms

Tethering-induced destabilization and ATP-binding for tandem RRM domains of ALS-causing TDP-43 and hnRNPA1

(2021) Mei Dang et al.   Scientific Reports

ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain

(2021) Mei Dang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

ALS-causing D169G mutation disrupts the ATP-binding capacity of TDP-43 RRM1 domain

(2020) Mei Dang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A new coronavirus associated with human respiratory disease in China

(2020) Fan Wu et al.   NATURE

Cataract-causing G18V eliminates the antagonization by ATP against the crowding-induced destabilization of human γS-crystallin

(2020) Yuan He et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ATP antagonizes the crowding-induced destabilization of the human eye-lens protein γS-crystallin

(2020) Yuan He et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Hydroxychloroquine use against SARS-CoV-2 infection in non-human primates

(2020) Pauline Maisonnasse et al.   NATURE

The possible mechanisms of action of 4-aminoquinolines (chloroquine/hydroxychloroquine) against Sars-Cov-2 infection (COVID-19): A role for iron homeostasis?

(2020) Eugenia Quiros Roldan et al.   PHARMACOLOGICAL RESEARCH

ATP differentially antagonizes the crowding-induced destabilization of human γS-crystallin and its four cataract-causing mutants

(2020) Yuan He et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2

(2020) Luca Zinzula et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

SARS‐CoV‐2 nucleocapsid protein phase‐separates with RNA and with human hnRNPs

(2020) Theodora Myrto Perdikari et al.   EMBO JOURNAL

Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions

(2020) Christopher R. Carlson et al.   MOLECULAR CELL

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

(2020) Adriana Savastano et al.   Nature Communications

Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein

(2020) Dhurvas Chandrasekaran Dinesh et al.   PLoS Pathogens

Proteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP

(2019) Sindhuja Sridharan et al.   Nature Communications

Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death

(2019) Fatima Gasset-Rosa et al.   NEURON

The role of liquid-liquid phase separation in aggregation of the TDP-43 low complexity domain

(2019) W. Michael Babinchak et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A novel mechanism for ATP to enhance the functional oligomerization of TDP-43 by specific binding

(2019) Lu Wang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The regulation, functions and clinical relevance of arginine methylation

(2019) Ernesto Guccione et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cryo-EM structures of four polymorphic TDP-43 amyloid cores

(2019) Qin Cao et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A unified mechanism for LLPS of ALS/FTLD-causing FUS as well as its modulation by ATP and oligonucleic acids

(2019) Jian Kang et al.   PLOS BIOLOGY

ATP binds nucleic-acid-binding domains beyond RRM fold

(2019) Yuan He et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ATP is a cryptic binder of TDP-43 RRM domains to enhance stability and inhibit ALS/AD-associated fibrillation

(2019) Mei Dang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The Landscape of Atypical and Eukaryotic Protein Kinases

(2019) Georgi K. Kanev et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Hydrotropic function of ATP in the crystalline lens

(2019) Jack V. Greiner et al.   EXPERIMENTAL EYE RESEARCH

TDP-43 NTD can be induced while CTD is significantly enhanced by ssDNA to undergo liquid-liquid phase separation

(2018) Lu Wang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Physical Principles and Extant Biology Reveal Roles for RNA-Containing Membraneless Compartments in Origins of Life Chemistry

(2018) Raghav R. Poudyal et al.   BIOCHEMISTRY

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

TAR DNA-binding protein 43 (TDP-43) liquid–liquid phase separation is mediated by just a few aromatic residues

(2018) Hao-Ru Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

RGG/RG Motif Regions in RNA Binding and Phase Separation

(2018) P. Andrew Chong et al.   JOURNAL OF MOLECULAR BIOLOGY

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Dual roles for ATP in the regulation of phase separated protein aggregates in Xenopus oocyte nucleoli

(2018) Michael H Hayes et al.   eLife

ATP enhances at low concentrations but dissolves at high concentrations liquid-liquid phase separation (LLPS) of ALS/FTD-causing FUS

(2018) Jian Kang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

ATP controls the crowd

(2017) Allyson M. Rice et al.   SCIENCE

ATP as a biological hydrotrope

(2017) Avinash Patel et al.   SCIENCE

Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation

(2017) Tariq Afroz et al.   Nature Communications

RRM domain of ALS/FTD-causing FUS characteristic of irreversible unfolding spontaneously self-assembles into amyloid fibrils

(2017) Yimei Lu et al.   Scientific Reports

Stability of Protein-Specific Hydration Shell on Crowding

(2016) Kuo-Ying Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Decoding ALS: from genes to mechanism

(2016) J. Paul Taylor et al.   NATURE

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain

(2016) Alexander E. Conicella et al.   STRUCTURE

In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes

(2016) Hermann Broder Schmidt et al.   Cell Reports

ALS-Causing Mutations Significantly Perturb the Self-Assembly and Interaction with Nucleic Acid of the Intrinsically Disordered Prion-Like Domain of TDP-43

(2016) Liangzhong Lim et al.   PLOS BIOLOGY

Distinct Role of Hydration Water in Protein Misfolding and Aggregation Revealed by Fluctuating Thermodynamics Analysis

(2015) Song-Ho Chong et al.   ACCOUNTS OF CHEMICAL RESEARCH

Biochemical Properties and Biological Functions of FET Proteins

(2015) Jacob C. Schwartz et al.   Annual Review of Biochemistry

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

(2015) Kathleen A. Burke et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Classification of Intrinsically Disordered Regions and Proteins

(2014) Robin van der Lee et al.   CHEMICAL REVIEWS

Polyphosphate Is a Primordial Chaperone

(2014) Michael J. Gray et al.   MOLECULAR CELL

Denatured Mammalian Protein Mixtures Exhibit Unusually High Solubility in Nucleic Acid-Free Pure Water

(2014) Junichiro Futami et al.   PLoS One

Dynamic hydration shell restores Kauzmann's 1959 explanation of how the hydrophobic factor drives protein folding

(2014) R. L. Baldwin   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TDP-43 N terminus encodes a novel ubiquitin-like fold and its unfolded form in equilibrium that can be shifted by binding to ssDNA

(2014) Haina Qin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Defining the RGG/RG Motif

(2013) Palaniraja Thandapani et al.   MOLECULAR CELL

Molecular basis of UG-rich RNA recognition by the human splicing factor TDP-43

(2013) Peter J Lukavsky et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Converging Mechanisms in ALS and FTD: Disrupted RNA and Protein Homeostasis

(2013) Shuo-Chien Ling et al.   NEURON

Solution properties of γ-crystallins: Hydration of fish and mammal γ-crystallins

(2013) Huaying Zhao et al.   PROTEIN SCIENCE

Why nature really chose phosphate

(2013) Shina C. L. Kamerlin et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Preferential and Specific Binding of Human αB-Crystallin to a Cataract-Related Variant of γS-Crystallin

(2013) Carolyn N. Kingsley et al.   STRUCTURE

Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies

(2012) Tina W. Han et al.   CELL

RGG motif proteins: Modulators of mRNA functional states

(2012) Purusharth Rajyaguru et al.   CELL CYCLE

Protein Crowding Affects Hydration Structure and Dynamics

(2012) Ryuhei Harada et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective and specific ion binding on proteins at physiologically-relevant concentrations

(2011) Linlin Miao et al.   FEBS LETTERS

More than one dynamic crossover in protein hydration water

(2011) M. G. Mazza et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Basic Helix−Loop−Helix Region of Human Neurogenin 1 Is a Monomeric Natively Unfolded Protein Which Forms a “Fuzzy” Complex upon DNA Binding

(2010) David Aguado-Llera et al.   BIOCHEMISTRY

Hydration properties of adenosine phosphate series as studied by microwave dielectric spectroscopy

(2010) George Mogami et al.   BIOPHYSICAL CHEMISTRY

SnapShot: Key Numbers in Biology

(2010) Uri Moran et al.   CELL

Why did Nature select phosphate for its dominant roles in biology?

(2010) Matthew W. Bowler et al.   NEW JOURNAL OF CHEMISTRY

Principles of protein folding - A perspective from simple exact models

(2010) Ken A. Dill et al.   PROTEIN SCIENCE

Lessons from the lysozyme of phage T4

(2010) Walter A. Baase et al.   PROTEIN SCIENCE

Proinsulin misfolding and diabetes: mutant INS gene-induced diabetes of youth

(2010) Ming Liu et al.   TRENDS IN ENDOCRINOLOGY AND METABOLISM

Inorganic Polyphosphate: Essential for Growth and Survival

(2009) Narayana N. Rao et al.   Annual Review of Biochemistry

The Tooth Enamel Protein, Porcine Amelogenin, Is an Intrinsically Disordered Protein with an Extended Molecular Configuration in the Monomeric Form†

(2009) Katya Delak et al.   BIOCHEMISTRY

Insight into “insoluble proteins” with pure water

(2009) Jianxing Song   FEBS LETTERS

SGX523 is an exquisitely selective, ATP-competitive inhibitor of the MET receptor tyrosine kinase with antitumor activity in vivo

(2009) S. G. Buchanan et al.   MOLECULAR CANCER THERAPEUTICS

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

(2009) Kohsuke Inomata et al.   NATURE

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Intracellular Protein Aggregation Is a Proximal Trigger of Cardiomyocyte Autophagy

(2008) Paul Tannous et al.   CIRCULATION

Water — an enduring mystery

(2008) Philip Ball   NATURE

Protein Phase Behavior in Aqueous Solutions: Crystallization, Liquid-Liquid Phase Separation, Gels, and Aggregates

(2007) André C. Dumetz et al.   BIOPHYSICAL JOURNAL

Water as an Active Constituent in Cell Biology

(2007) Philip Ball   CHEMICAL REVIEWS