Progression and application of CRISPR-Cas genomic editors

DNA Prime Editing: A New CRISPR-Based Method to Correct Most Disease-Causing Mutations

(2020) Tracy Hampton   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors

(2020) Jordan L. Doman et al.   NATURE BIOTECHNOLOGY

Continuous evolution of SpCas9 variants compatible with non-G PAMs

(2020) Shannon M. Miller et al.   NATURE BIOTECHNOLOGY

Programmable base editing of mutated TERT promoter inhibits brain tumour growth

(2020) Xinjian Li et al.   NATURE CELL BIOLOGY

Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses

(2020) Jonathan M. Levy et al.   Nature Biomedical Engineering

Versatile Nucleotides Substitution in Plant Using an Improved Prime Editing System

(2020) Wen Xu et al.   Molecular Plant

Directed evolution of adenine base editors with increased activity and therapeutic application

(2020) Nicole M. Gaudelli et al.   NATURE BIOTECHNOLOGY

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

(2020) Michelle F. Richter et al.   NATURE BIOTECHNOLOGY

Dual base editor catalyzes both cytosine and adenine base conversions in human cells

(2020) Xiaohui Zhang et al.   NATURE BIOTECHNOLOGY

Base editors for simultaneous introduction of C-to-T and A-to-G mutations

(2020) Rina C. Sakata et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain

(2020) Xiaohui Zhang et al.   NATURE CELL BIOLOGY

A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects

(2020) Erwei Zuo et al.   NATURE METHODS

Precision genome engineering in rice using prime editing system

(2020) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplex precise base editing in cynomolgus monkeys

(2020) Wenhui Zhang et al.   Nature Communications

Efficient generation of mouse models with the prime editing system

(2020) Yao Liu et al.   Cell Discovery

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Base editing with high efficiency in allotetraploid oilseed rape by A3A‐PBE system

(2020) Hongtao Cheng et al.   PLANT BIOTECHNOLOGY JOURNAL

In vivo base editing restores sensory transduction and transiently improves auditory function in a mouse model of recessive deafness

(2020) Wei-Hsi Yeh et al.   Science Translational Medicine

Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering

(2020) Yu Wang et al.   TRENDS IN BIOTECHNOLOGY

Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion

(2020) Akira Komatsu et al.   Frontiers in Plant Science

CRISPR/Cas mediated base editing: a practical approach for genome editing in oil palm

(2020) Rajesh Yarra et al.   3 Biotech

Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects

(2020) Sangsin Lee et al.   Science Advances

BE4max and AncBE4max Are Efficient in Germline Conversion of C:G to T:A Base Pairs in Zebrafish

(2020) Blake Carrington et al.   Cells

AcrIIA5 Suppresses Base Editors and Reduces Their Off-Target Effects

(2020) Mingming Liang et al.   Cells

Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

(2020) Zhiquan Liu et al.   BMC BIOLOGY

ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems

(2020) Jingke Xie et al.   BMC BIOLOGY

Highly elevated base excision repair pathway in primordial germ cells causes low base editing activity in chickens

(2020) Kyung Youn Lee et al.   FASEB JOURNAL

Precise Genome Modification in Tomato Using an Improved Prime Editing System

(2020) Yuming Lu et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing

(2020) Minh Thuan Nguyen Tran et al.   Nature Communications

Restoration of visual function in adult mice with an inherited retinal disease via adenine base editing

(2020) Susie Suh et al.   Nature Biomedical Engineering

Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease

(2020) Tao Qi et al.   Frontiers in Cell and Developmental Biology

Genome editing systems across yeast species

(2020) Zhiliang Yang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination

(2020) Shuo Li et al.   Nature Communications

Genome-wide profiling of adenine base editor specificity by EndoV-seq

(2019) Puping Liang et al.   Nature Communications

Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations

(2019) Shiwei Zhou et al.   Frontiers in Genetics

Engineering of high-precision base editors for site-specific single nucleotide replacement

(2019) Junjie Tan et al.   Nature Communications

Adenine base editing in an adult mouse model of tyrosinaemia

(2019) Chun-Qing Song et al.   Nature Biomedical Engineering

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

(2019) Tony P. Huang et al.   NATURE BIOTECHNOLOGY

An adenine base editor with expanded targeting scope using SpCas9‐ NG v1 in rice

(2019) Katsuya Negishi et al.   PLANT BIOTECHNOLOGY JOURNAL

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice

(2019) Ying Wu et al.   Frontiers in Genetics

Analysis and minimization of cellular RNA editing by DNA adenine base editors

(2019) Holly A. Rees et al.   Science Advances

Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7)

(2019) Zhiquan Liu et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Base pair editing in goat: nonsense codon introgression into FGF 5 results in longer hair

(2019) Guanwei Li et al.   FEBS Journal

Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis

(2019) Changyang Zhou et al.   NATURE

Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair

(2019) Wei Chen et al.   NUCLEIC ACIDS RESEARCH

Efficient base editing for multiple genes and loci in pigs using base editors

(2019) Jingke Xie et al.   Nature Communications

Efficient and precise base editing in rabbits using human APOBEC3A-nCas9 fusions

(2019) Zhiquan Liu et al.   Cell Discovery

CRISPR DNA base editors with reduced RNA off-target and self-editing activities

(2019) Julian Grünewald et al.   NATURE BIOTECHNOLOGY

Simplified adenine base editors improve adenine base editing efficiency in rice

(2019) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeted exon skipping with AAV-mediated split adenine base editors

(2019) Jackson Winter et al.   Cell Discovery

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity

(2018) Wen Jiang et al.   CELL RESEARCH

Mycobacterium tuberculosis type III-A CRISPR/Cas system crRNA and its maturation have atypical features

(2018) Wenjing Wei et al.   FASEB JOURNAL

Precise A·T to G·C Base Editing in the Rice Genome

(2018) Kai Hua et al.   Molecular Plant

Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy

(2018) Seuk-Min Ryu et al.   NATURE BIOTECHNOLOGY

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

Highly efficient RNA-guided base editing in rabbit

(2018) Zhiquan Liu et al.   Nature Communications

Progress in the application of CRISPR: From gene to base editing

(2018) Wenyi Wu et al.   MEDICINAL RESEARCH REVIEWS

Expanding the base editing scope in rice by using Cas9 variants

(2018) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

In vivo targeted single-nucleotide editing in zebrafish

(2018) Shingo Tanaka et al.   Scientific Reports

Precise A•T to G•C base editing in the zebrafish genome

(2018) Wei Qin et al.   BMC BIOLOGY

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Synthetic AAV/CRISPR vectors for blocking HIV-1 expression in persistently infected astrocytes

(2017) Christine Kunze et al.   GLIA

APOBEC: From mutator to editor

(2017) Bei Yang et al.   Journal of Genetics and Genomics

Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish

(2017) Shahad Albadri et al.   METHODS

Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum

(2017) Jiao Liu et al.   Microbial Cell Factories

CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

(2017) Pierre Billon et al.   MOLECULAR CELL

Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations

(2017) Cem Kuscu et al.   NATURE METHODS

Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress

(2017) Chantal LeBlanc et al.   PLANT JOURNAL

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

(2017) Yihan Zhang et al.   Nature Communications

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

(2017) Holly A. Rees et al.   Nature Communications

Highly efficient and precise base editing in discarded human tripronuclear embryos

(2017) Guanglei Li et al.   Protein & Cell

Correction of β-thalassemia mutant by base editor in human embryos

(2017) Puping Liang et al.   Protein & Cell

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

(2017) Puping Liang et al.   Protein & Cell

Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity

(2017) Alexis C. Komor et al.   Science Advances

CRISPR-Mediated Base Editing without DNA Double-Strand Breaks

(2016) Brian S. Plosky   MOLECULAR CELL

In Vivo CRISPR/Cas9 Gene Editing Corrects Retinal Dystrophy in the S334ter-3 Rat Model of Autosomal Dominant Retinitis Pigmentosa

(2016) Benjamin Bakondi et al.   MOLECULAR THERAPY

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool

(2016) Cem Kuscu et al.   NATURE METHODS

Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

(2016) Qiupeng Zheng et al.   BIOTECHNIQUES

CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus

(2015) Dipankan Bhattacharya et al.   DEVELOPMENTAL BIOLOGY

ClinVar: public archive of interpretations of clinically relevant variants

(2015) Melissa J. Landrum et al.   NUCLEIC ACIDS RESEARCH

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification

(2014) Syandan Chakraborty et al.   Stem Cell Reports

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus

(2013) Hirotaka Ebina et al.   Scientific Reports

Hydrodynamic Limb Vein Injection of Adeno-Associated Virus Serotype 8 Vector Carrying Canine Myostatin Propeptide Gene into Normal Dogs Enhances Muscle Growth

(2009) Chunping Qiao et al.   HUMAN GENE THERAPY