Phosphorylation-dependent regulation of messenger RNA transcription, processing and translation within biomolecular condensates

Modulation of RNA Condensation by the DEAD-Box Protein eIF4A

(2020) Devin Tauber et al.   CELL

Distinct features of stress granule proteins predict localization in membraneless organelle

(2020) Erich R. Kuechler et al.   JOURNAL OF MOLECULAR BIOLOGY

Arginine-Enriched Mixed-Charge Domains Provide Cohesion for Nuclear Speckle Condensation

(2020) Jamie A. Greig et al.   MOLECULAR CELL

Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins

(2020) Chih-Yung S Lee et al.   eLife

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization

(2020) David W. Sanders et al.   CELL

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Regulation of RNA granules by FMRP and implications for neurological diseases

(2020) Austin Lai et al.   TRAFFIC

CDK-Regulated Phase Separation Seeded by Histone Genes Ensures Precise Growth and Function of Histone Locus Bodies

(2020) Woonyung Hur et al.   DEVELOPMENTAL CELL

Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis

(2020) Wei Li et al.   NATURE CELL BIOLOGY

Biomolecular Condensates in the Nucleus

(2020) Benjamin R. Sabari et al.   TRENDS IN BIOCHEMICAL SCIENCES

Liquid- and solid-like RNA granules form through specific scaffold proteins and combine into biphasic granules

(2019) Nobuyuki Shiina   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphoregulated FMRP phase separation models activity-dependent translation through bidirectional control of mRNA granule formation

(2019) Brian Tsang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos

(2019) Andrea Putnam et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The C-Terminal Domain of RNA Polymerase II Is a Multivalent Targeting Sequence that Supports Drosophila Development with Only Consensus Heptads

(2019) Feiyue Lu et al.   MOLECULAR CELL

The Long and the Short of the RNA Polymerase C-Terminal Domain and Phase Separation

(2019) R. Stefan Isaac et al.   MOLECULAR CELL

Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition

(2019) Jiah Kim et al.   JOURNAL OF CELL SCIENCE

Regulation of Transmembrane Signaling by Phase Separation

(2019) Lindsay B. Case et al.   Annual Review of Biophysics

ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97

(2019) Bo Wang et al.   MOLECULAR CELL

C9orf72 Poly(PR) Dipeptide Repeats Disturb Biomolecular Phase Separation and Disrupt Nucleolar Function

(2019) Michael R. White et al.   MOLECULAR CELL

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

U2 AF 65 assemblies drive sequence‐specific splice site recognition

(2019) Manel Tari et al.   EMBO REPORTS

Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells

(2019) Joseph R. Simon et al.   MOLECULAR CELL

Chromatin topology, condensates and gene regulation: shifting paradigms or just a phase?

(2019) Mustafa Mir et al.   DEVELOPMENT

Mediator Condensates Localize Signaling Factors to Key Cell Identity Genes

(2019) Alicia V. Zamudio et al.   MOLECULAR CELL

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

(2019) Yang Eric Guo et al.   NATURE

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

(2019) Tae Hun Kim et al.   SCIENCE

Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching

(2019) Nicole O. Taylor et al.   BIOPHYSICAL JOURNAL

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

Properties of Stress Granule and P-Body Proteomes

(2019) Ji-Young Youn et al.   MOLECULAR CELL

The prion-like protein kinase Sky1 is required for efficient stress granule disassembly

(2019) Jenifer E. Shattuck et al.   Nature Communications

Relationship of Sequence and Phase Separation in Protein Low-Complexity Regions

(2018) Erik W. Martin et al.   BIOCHEMISTRY

Do Cellular Condensates Accelerate Biochemical Reactions? Lessons from Microdroplet Chemistry

(2018) Wylie Stroberg et al.   BIOPHYSICAL JOURNAL

Phase Transitions in the Assembly and Function of Human miRISC

(2018) Jessica Sheu-Gruttadauria et al.   CELL

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus

(2018) Sofia A. Quinodoz et al.   CELL

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

mTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding

(2018) M. Delarue et al.   CELL

A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing

(2018) Ailin Wang et al.   EMBO JOURNAL

Tau protein liquid–liquid phase separation can initiate tau aggregation

(2018) Susanne Wegmann et al.   EMBO JOURNAL

Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules

(2018) Benedikt Niewidok et al.   JOURNAL OF CELL BIOLOGY

Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation

(2018) Tomohiro Yamazaki et al.   MOLECULAR CELL

RNA self-assembly contributes to stress granule formation and defining the stress granule transcriptome

(2018) Briana Van Treeck et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

(2018) Steven Boeynaems et al.   TRENDS IN CELL BIOLOGY

P-Bodies: Cytosolic Droplets for Coordinated mRNA Storage

(2018) Nancy Standart et al.   TRENDS IN GENETICS

Pi-Pi contacts are an overlooked protein feature relevant to phase separation

(2018) Robert McCoy Vernon et al.   eLife

The P Granules of C. elegans: A Genetic Model for the Study of RNA–Protein Condensates

(2018) Geraldine Seydoux   JOURNAL OF MOLECULAR BIOLOGY

Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II

(2018) Huasong Lu et al.   NATURE

Kinase-controlled phase transition of membraneless organelles in mitosis

(2018) Arpan Kumar Rai et al.   NATURE

RNA polymerase II clustering through carboxy-terminal domain phase separation

(2018) Marc Boehning et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Stress Granules and Processing Bodies in Translational Control

(2018) Pavel Ivanov et al.   Cold Spring Harbor Perspectives in Biology

Compartmentalised RNA catalysis in membrane-free coacervate protocells

(2018) Björn Drobot et al.   Nature Communications

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Friend or foe — post-translational modifications as regulators of phase separation and RNP granule dynamics

(2018) Mario Hofweber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain

(2017) Kevin M. Harlen et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping

(2017) Stefan Schütz et al.   NUCLEIC ACIDS RESEARCH

Nuclear speckles: molecular organization, biological function and role in disease

(2017) Lukasz Galganski et al.   NUCLEIC ACIDS RESEARCH

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Dynamic Control of Dendritic mRNA Expression by CNOT7 Regulates Synaptic Efficacy and Higher Cognitive Function

(2017) Rhonda L. McFleder et al.   Cell Reports

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

(2016) Saumya Jain et al.   CELL

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

Sequence Determinants of the Conformational Properties of an Intrinsically Disordered Protein Prior to and upon Multisite Phosphorylation

(2016) Erik W. Martin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Casein Kinase 2 Is Linked to Stress Granule Dynamics through Phosphorylation of the Stress Granule Nucleating Protein G3BP1

(2016) Lucas C. Reineke et al.   MOLECULAR AND CELLULAR BIOLOGY

CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets

(2016) Jordina Guillén-Boixet et al.   eLife

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Remote Control of Gene Function by Local Translation

(2014) Hosung Jung et al.   CELL

Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells

(2014) I. Kwon et al.   SCIENCE

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

(2014) Jennifer T Wang et al.   eLife

Phosphorylation-Regulated Binding of RNA Polymerase II to Fibrous Polymers of Low-Complexity Domains

(2013) Ilmin Kwon et al.   CELL

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Polycation-π Interactions Are a Driving Force for Molecular Recognition by an Intrinsically Disordered Oncoprotein Family

(2013) Jianhui Song et al.   PLoS Computational Biology

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Cell-free Formation of RNA Granules: Bound RNAs Identify Features and Components of Cellular Assemblies

(2012) Tina W. Han et al.   CELL

Evidence of the Involvement ofO-GlcNAc-modified Human RNA Polymerase II CTD in Transcriptionin Vitroandin Vivo

(2012) Stella M. Ranuncolo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

RNA catalysis through compartmentalization

(2012) Christopher A. Strulson et al.   Nature Chemistry

Nuclear Speckles

(2010) D. L. Spector et al.   Cold Spring Harbor Perspectives in Biology

Multifunctional deadenylase complexes diversify mRNA control

(2008) Aaron C. Goldstrohm et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY