A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli

Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

(2020) Buli Su et al.   Microbial Cell Factories

Determinants for Efficient Editing with Cas9-Mediated Recombineering in Escherichia coli

(2020) Alaksh Choudhury et al.   ACS Synthetic Biology

Combining Orthogonal CRISPR and CRISPRi Systems for Genome Engineering and Metabolic Pathway Modulation in E. coli

(2019) Li-Yu Sung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Simple and efficient genome recombineering using kil counter-selection in Escherichia coli

(2019) Wei Chen et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli

(2019) Chaoyong Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli

(2018) Xu Feng et al.   Biotechnology Journal

Escherichia coli as a host for metabolic engineering

(2018) Sammy Pontrelli et al.   METABOLIC ENGINEERING

CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli

(2018) Xu Feng et al.   Biotechnology Journal

Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli

(2018) Yanjun Li et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a

(2018) Xiang Ao et al.   Frontiers in Microbiology

Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

(2017) Francesca Zerbini et al.   Microbial Cell Factories

Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity

(2017) Eirik Adim Moreb et al.   ACS Synthetic Biology

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

(2015) Chris R. Reisch et al.   Scientific Reports

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli

(2011) Colin T Archer et al.   BMC GENOMICS

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols