The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites

(2015) Irene M. Brockman et al.   METABOLIC ENGINEERING

A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

(2014) Juhan Kim et al.   BMC BIOTECHNOLOGY

Harnessing CRISPR-Cas9 immunity for genetic engineering

(2014) Emmanuelle Charpentier et al.   CURRENT OPINION IN MICROBIOLOGY

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Bacterial DNA polymerases participate in oligonucleotide recombination

(2013) Xin-tian Li et al.   MOLECULAR MICROBIOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

(2013) Xin-tian Li et al.   NUCLEIC ACIDS RESEARCH

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

Genome-scale promoter engineering by coselection MAGE

(2012) Harris H Wang et al.   NATURE METHODS

Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection

(2012) Peter A. Carr et al.   NUCLEIC ACIDS RESEARCH

Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering

(2012) M. J. Lajoie et al.   NUCLEIC ACIDS RESEARCH

EcoCyc: fusing model organism databases with systems biology

(2012) Ingrid M. Keseler et al.   NUCLEIC ACIDS RESEARCH

FRUIT, a Scar-Free System for Targeted Chromosomal Mutagenesis, Epitope Tagging, and Promoter Replacement in Escherichia coli and Salmonella enterica

(2012) Anne M. Stringer et al.   PLoS One

Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

(2012) Joshua A. Mosberg et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Preparation of highly efficient electrocompetent Escherichia coli using glycerol/mannitol density step centrifugation

(2011) David J. Warren   ANALYTICAL BIOCHEMISTRY

Probing Cellular Processes with Oligo-Mediated Recombination and Using the Knowledge Gained to Optimize Recombineering

(2011) James A. Sawitzke et al.   JOURNAL OF MOLECULAR BIOLOGY

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Single-stranded heteroduplex intermediates in λ Red homologous recombination

(2010) Marcello Maresca et al.   BMC MOLECULAR BIOLOGY

Lambda Red Recombineering in Escherichia coli Occurs Through a Fully Single-Stranded Intermediate

(2010) J. A. Mosberg et al.   GENETICS

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

A simple and fast method for determining colony forming units

(2008) S. Sieuwerts et al.   LETTERS IN APPLIED MICROBIOLOGY

Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination

(2008) Ralf Heermann et al.   Microbial Cell Factories

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

(2008) B. J. Yu et al.   NUCLEIC ACIDS RESEARCH