CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Dynamics of RecA-mediated repair of replication-dependent DNA breaks

(2018) Vincent Amarh et al.   JOURNAL OF CELL BIOLOGY

Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-Mediated Nucleotide Insertion

(2018) Jia Shou et al.   MOLECULAR CELL

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

(2018) Suhyung Cho et al.   ACS Synthetic Biology

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis

(2018) Yongzhen Xia et al.   NUCLEIC ACIDS RESEARCH

A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette

(2017) Hong Zhang et al.   Frontiers in Microbiology

CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency

(2017) Dongdong Zhao et al.   Scientific Reports

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

(2016) Dongdong Zhao et al.   Microbial Cell Factories

Mycobacterium tuberculosisandMycobacterium marinumnon-homologous end-joining proteins can function together to join DNA ends inEscherichia coli

(2016) Douglas G. Wright et al.   MUTAGENESIS

Synthetic biology to access and expand nature's chemical diversity

(2016) Michael J. Smanski et al.   NATURE REVIEWS MICROBIOLOGY

Consequences of Cas9 cleavage in the chromosome ofEscherichia coli

(2016) Lun Cui et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

CRMAGE: CRISPR Optimized MAGE Recombineering

(2016) Carlotta Ronda et al.   Scientific Reports

A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

(2016) Tianyuan Su et al.   Scientific Reports

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Bacterial Recombineering: Genome Engineering via Phage-Based Homologous Recombination

(2015) Gur Pines et al.   ACS Synthetic Biology

A rapid and reliable strategy for chromosomal integration of gene(s) with multiple copies

(2015) Pengfei Gu et al.   Scientific Reports

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Codon Compression Algorithms for Saturation Mutagenesis

(2014) Gur Pines et al.   ACS Synthetic Biology

Genome-scale engineering for systems and synthetic biology

(2013) K. M. Esvelt et al.   Molecular Systems Biology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Improved seamless mutagenesis by recombineering using ccdB for counterselection

(2013) Hailong Wang et al.   NUCLEIC ACIDS RESEARCH

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

(2013) Christine Nicole S. Santos et al.   Nature Communications

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

An end-joining repair mechanism in Escherichia coli

(2010) R. Chayot et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE