A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a

CRISPR/Cas9 editing genome of extremophile Halomonas spp.

(2018) Qin Qin et al.   METABOLIC ENGINEERING

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

(2017) Yu Jiang et al.   Nature Communications

Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in E. coli

(2017) Jacob A. Englaender et al.   ACS Synthetic Biology

A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette

(2017) Hong Zhang et al.   Frontiers in Microbiology

Enhanced integration of large DNA intoE. colichromosome by CRISPR/Cas9

(2016) Mu-En Chung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

(2016) Dongdong Zhao et al.   Microbial Cell Factories

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

(2016) Fillip Port et al.   NATURE METHODS

Consequences of Cas9 cleavage in the chromosome ofEscherichia coli

(2016) Lun Cui et al.   NUCLEIC ACIDS RESEARCH

Microbial Synthesis of 5-Aminolevulinic Acid and Its Coproduction with Polyhydroxybutyrate

(2016) Tian Li et al.   ACS Synthetic Biology

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli

(2015) Chris R. Reisch et al.   Scientific Reports

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Microbial production and applications of 5-aminolevulinic acid

(2014) Shuli Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Generalized bacterial genome editing using mobile group II introns and Cre-lox

(2013) P. J. Enyeart et al.   Molecular Systems Biology

Genome-scale engineering for systems and synthetic biology

(2013) K. M. Esvelt et al.   Molecular Systems Biology

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Comparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships

(2011) Lei Cai et al.   Microbial Cell Factories

Developing an Extended Genomic Engineering Approach Based on Recombineering to Knock-in Heterologous Genes to Escherichia coli Genome

(2011) Karan Sukhija et al.   MOLECULAR BIOTECHNOLOGY

Regulation of transcription by unnatural amino acids

(2011) Chang C Liu et al.   NATURE BIOTECHNOLOGY

An end-joining repair mechanism in Escherichia coli

(2010) R. Chayot et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Recombineering: a homologous recombination-based method of genetic engineering

(2009) Shyam K Sharan et al.   Nature Protocols

Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination

(2008) Ralf Heermann et al.   Microbial Cell Factories

Rapid and efficient construction of markerless deletions in the Escherichia coli genome

(2008) B. J. Yu et al.   NUCLEIC ACIDS RESEARCH