The CRISPR-Cas Mechanism for Adaptive Immunity and Alternate Bacterial Functions Fuels Diverse Biotechnologies

Specificity profiling of CRISPR system reveals greatly enhanced off-target gene editing

(2020) Yao Wang et al.   Scientific Reports

CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers

(2020) Franziska Wimmer et al.   Frontiers in Microbiology

CRISPR-Cas12a: Functional overview and applications

(2020) Bijoya Paul et al.   Biomedical Journal

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

(2020) Nicole D. Marino et al.   NATURE METHODS

A short overview of the CRISPR-Cas adaptation stage

(2020) Cas Mosterd et al.   CANADIAN JOURNAL OF MICROBIOLOGY

A scoutRNA Is Required for Some Type V CRISPR-Cas Systems

(2020) Lucas B. Harrington et al.   MOLECULAR CELL

Guide-free Cas9 from pathogenic Campylobacter jejuni bacteria causes severe damage to DNA

(2020) Chinmoy Saha et al.   Science Advances

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation

(2020) Tina Y. Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Campylobacter jejuni Cas9 Modulates the Transcriptome in Caco-2 Intestinal Epithelial Cells

(2020) Chinmoy Saha et al.   Genes

Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits

(2019) Irmantas Mogila et al.   Cell Reports

Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity

(2019) Boyu Tang et al.   ARCHIVES OF ORAL BIOLOGY

Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

(2019) Adam E. Dolan et al.   MOLECULAR CELL

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

(2019) Lucy Chou-Zheng et al.   eLife

Catalytically Active Cas9 Mediates Transcriptional Interference to Facilitate Bacterial Virulence

(2019) Hannah K. Ratner et al.   MOLECULAR CELL

Structural basis of Type IV CRISPR RNA biogenesis by a Cas6 endoribonuclease

(2019) Hannah N. Taylor et al.   RNA Biology

Cas9 Contributes to Group B Streptococcal Colonization and Disease

(2019) Brady L. Spencer et al.   Frontiers in Microbiology

Functional and Proteomic Analysis of Streptococcus pyogenes Virulence Upon Loss of Its Native Cas9 Nuclease

(2019) Nina J. Gao et al.   Frontiers in Microbiology

Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer

(2019) Ying Wang et al.   MICROBIAL PATHOGENESIS

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

(2019) Peter Cameron et al.   NATURE BIOTECHNOLOGY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids

(2019) Rafael Pinilla-Redondo et al.   NUCLEIC ACIDS RESEARCH

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

A CRISPR–dCas Toolbox for Genetic Engineering and Synthetic Biology

(2018) Xiaoshu Xu et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR–Cas Systems

(2018) Mitchell R. O'Connell   JOURNAL OF MOLECULAR BIOLOGY

Base editing with a Cpf1–cytidine deaminase fusion

(2018) Xiaosa Li et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR-Cas-Mediated Phage Resistance Enhances Horizontal Gene Transfer by Transduction

(2018) Bridget N. J. Watson et al.   mBio

RNA-dependent RNA targeting by CRISPR-Cas9

(2018) Steven C Strutt et al.   eLife

CRISPR–Cas: Complex Functional Networks and Multiple Roles beyond Adaptive Immunity

(2018) Guilhem Faure et al.   JOURNAL OF MOLECULAR BIOLOGY

PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures

(2018) Daniel Gleditzsch et al.   RNA Biology

The CRISPR/Cas system in Neisseria meningitidis affects bacterial adhesion to human nasopharyngeal epithelial cells

(2018) Nadja Heidrich et al.   RNA Biology

The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni

(2018) Muhammad A. B. Shabbir et al.   Frontiers in Cellular and Infection Microbiology

Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference

(2018) Lilan You et al.   CELL

Multiplexed orthogonal genome editing and transcriptional activation by Cas12a

(2018) Marco Breinig et al.   NATURE METHODS

Functionally diverse type V CRISPR-Cas systems

(2018) Winston X. Yan et al.   SCIENCE

Type IV CRISPR RNA processing and effector complex formation in Aromatoleum aromaticum

(2018) Ahsen Özcan et al.   Nature Microbiology

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit

(2017) Karthik Murugan et al.   MOLECULAR CELL

Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers

(2017) Ole Niewoehner et al.   NATURE

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems

(2017) Migle Kazlauskiene et al.   SCIENCE

Type III CRISPR-Cas Immunity: Major Differences Brushed Aside

(2017) Gintautas Tamulaitis et al.   TRENDS IN MICROBIOLOGY

The CRISPR Spacer Space Is Dominated by Sequences from Species-Specific Mobilomes

(2017) Sergey A. Shmakov et al.   mBio

RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

(2017) Ramya Sundaresan et al.   Cell Reports

Multiplex gene regulation by CRISPR-ddCpf1

(2017) Xiaochun Zhang et al.   Cell Discovery

Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity

(2016) Rongpeng Li et al.   CELL RESEARCH

RNA-activated DNA cleavage by the Type III-B CRISPR–Cas effector complex

(2016) Michael A. Estrella et al.   GENES & DEVELOPMENT

Adaptation in CRISPR-Cas Systems

(2016) Samuel H. Sternberg et al.   MOLECULAR CELL

Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition

(2016) Migle Kazlauskiene et al.   MOLECULAR CELL

Regulatory gene mutation: a driving force behind group aStreptococcusstrain- and serotype-specific variation

(2016) Poulomee Sarkar et al.   MOLECULAR MICROBIOLOGY

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

New CRISPR–Cas systems from uncultivated microbes

(2016) David Burstein et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins

(2016) Junho K Hur et al.   NATURE BIOTECHNOLOGY

Multidimensional chemical control of CRISPR–Cas9

(2016) Basudeb Maji et al.   Nature Chemical Biology

Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein

(2016) S. Silas et al.   SCIENCE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity

(2015) Poulami Samai et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR adaptation biases explain preference for acquisition of foreign DNA

(2015) Asaf Levy et al.   NATURE

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Harnessing Type I and Type III CRISPR-Cas systems for genome editing

(2015) Yingjun Li et al.   NUCLEIC ACIDS RESEARCH

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning

(2015) D. W. Taylor et al.   SCIENCE

Clustered Regularly Interspaced Short Palindromic Repeat-Dependent, Biofilm-Specific Death of Pseudomonas aeruginosa Mediated by Increased Expression of Phage-Related Genes

(2015) Gary E. Heussler et al.   mBio

A CRISPR with Roles in Myxococcus xanthus Development and Exopolysaccharide Production

(2014) R. A. Wallace et al.   JOURNAL OF BACTERIOLOGY

RNA Targeting by the Type III-A CRISPR-Cas Csm Complex of Thermus thermophilus

(2014) Raymond H.J. Staals et al.   MOLECULAR CELL

Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting

(2014) Gregory W. Goldberg et al.   NATURE

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Efficient programmable gene silencing by Cascade

(2014) Devashish Rath et al.   NUCLEIC ACIDS RESEARCH

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus

(2014) Ziga Zebec et al.   NUCLEIC ACIDS RESEARCH

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Structure and Activity of the RNA-Targeting Type III-B CRISPR-Cas Complex of Thermus thermophilus

(2013) Raymond H.J. Staals et al.   MOLECULAR CELL

Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis

(2013) Yan Zhang et al.   MOLECULAR CELL

A CRISPR/Cas system mediates bacterial innate immune evasion and virulence

(2013) Timothy R. Sampson et al.   NATURE

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

Degeneration of a CRISPR/Cas system and its regulatory target during the evolution of a pathogen

(2013) Timothy R Sampson et al.   RNA Biology

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

The CRISPR-Associated Gene cas2 of Legionella pneumophila Is Required for Intracellular Infection of Amoebae

(2013) F. F. Gunderson et al.   mBio

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

A novel link between Campylobacter jejuni bacteriophage defence, virulence and Guillain–Barré syndrome

(2012) R. Louwen et al.   EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of Pelobacter carbinolicus

(2010) Muktak Aklujkar et al.   BMC EVOLUTIONARY BIOLOGY

A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair

(2010) Mohan Babu et al.   MOLECULAR MICROBIOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE