Synthetic Biology Approaches To Enhance Microalgal Productivity

A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation

(2021) Marieke Scheffen et al.   Nature Catalysis

Growth of E. coli on formate and methanol via the reductive glycine pathway

(2020) Seohyoung Kim et al.   Nature Chemical Biology

Extrachromosomal Genetic Engineering of the Marine Diatom Phaeodactylum tricornutum Enables the Heterologous Production of Monoterpenoids

(2020) Michele Fabris et al.   ACS Synthetic Biology

Overexpression of Chlamydomonas reinhardtii LCIA (CrLCIA) gene increases growth of Nannochloropsis salina CCMP1776

(2020) Jayaraman Vikramathithan et al.   Algal Research-Biomass Biofuels and Bioproducts

Potential and Challenges of Improving Photosynthesis in Algae

(2020) Valeria Vecchi et al.   Plants-Basel

Prospects for Engineering Biophysical CO2 Concentrating Mechanisms into Land Plants to Enhance Yields

(2020) Jessica H. Hennacy et al.   Annual Review of Plant Biology

Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts

(2020) Tarryn E. Miller et al.   SCIENCE

Doubling of Microalgae Productivity by Oxygen Balanced Mixotrophy

(2020) Fabian Abiusi et al.   ACS Sustainable Chemistry & Engineering

Highly active rubiscos discovered by systematic interrogation of natural sequence diversity

(2020) Dan Davidi et al.   EMBO JOURNAL

Genome editing using Cas9-RNA ribonucleoprotein complexes in the high-productivity marine alga Picochlorum celeri

(2020) Anagha Krishnan et al.   Algal Research-Biomass Biofuels and Bioproducts

Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica

(2020) Imke de Grahl et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle

(2020) Nico J. Claassens et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The reductive glycine pathway allows autotrophic growth of Desulfovibrio desulfuricans

(2020) Irene Sánchez-Andrea et al.   Nature Communications

Awakening a latent carbon fixation cycle in Escherichia coli

(2020) Ari Satanowski et al.   Nature Communications

Improving the efficiency of photosynthetic carbon reactions

(2019) Andreas P.M. Weber et al.   PLANT PHYSIOLOGY

Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field

(2019) Paul F. South et al.   SCIENCE

Application of the CRISPR/Cas system for genome editing in microalgae

(2019) Yu-Ting Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Heterologous synthesis of chlorophyll b in Nannochloropsis salina enhances growth and lipid production by increasing photosynthetic efficiency

(2019) Hyun Gi Koh et al.   Biotechnology for Biofuels

Core Catalysis of the Reductive Glycine Pathway Demonstrated in Yeast

(2019) Jorge Gonzalez de la Cruz et al.   ACS Synthetic Biology

Revisiting Trade-offs between Rubisco Kinetic Parameters

(2019) Avi I. Flamholz et al.   BIOCHEMISTRY

Effect of organic carbon to nitrogen ratio in wastewater on growth, nutrient uptake and lipid accumulation of a mixotrophic microalgae Chlorella sp.

(2019) Feng Gao et al.   BIORESOURCE TECHNOLOGY

Photorespiration in an outdoor alkaline open-photobioreactor used for biogas upgrading

(2019) Eliana Isabel Toro-Huertas et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Selection of Cyanobacterial (Synechococcus sp. Strain PCC 6301) RubisCO Variants with Improved Functional Properties That Confer Enhanced CO2-Dependent Growth of Rhodobacter capsulatus, a Photosynthetic Bacterium

(2019) Sriram Satagopan et al.   mBio

Making quantitative sense of electromicrobial production

(2019) Nico J. Claassens et al.   Nature Catalysis

Conversion of Escherichia coli to Generate All Biomass Carbon from CO2

(2019) Shmuel Gleizer et al.   CELL

Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction

(2019) Yu Zhou et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans

(2019) Camilo F. Muñoz et al.   Frontiers in Plant Science

The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2

(2019) Thomas Gassler et al.   NATURE BIOTECHNOLOGY

A high-capacity gene stacking toolkit for the oleaginous microalga, Nannochloropsis oceanica CCMP1779

(2019) Eric Poliner et al.   Algal Research-Biomass Biofuels and Bioproducts

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Towards sustainable feedstocks: A guide to electron donors for microbial carbon fixation

(2018) Nico Joannes Claassens et al.   CURRENT OPINION IN BIOTECHNOLOGY

Reinforcing carbon fixation: CO 2 reduction replacing and supporting carboxylation

(2018) Charles AR Cotton et al.   CURRENT OPINION IN BIOTECHNOLOGY

Energy input assessment for nanosecond pulsed electric field processing and its application in a case study with Chlorella vulgaris

(2018) Leandro Buchmann et al.   Innovative Food Science & Emerging Technologies

Constitutive and Chloroplast Targeted Expression of Acetyl-CoA Carboxylase in Oleaginous Microalgae Elevates Fatty Acid Biosynthesis

(2018) Da-Wei Li et al.   MARINE BIOTECHNOLOGY

Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002

(2018) Alice Jara De Porcellinis et al.   METABOLIC ENGINEERING

Unrestrained markerless trait stacking inNannochloropsis gaditanathrough combined genome editing and marker recycling technologies

(2018) John Verruto et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reversibility of citrate synthase allows autotrophic growth of a thermophilic bacterium

(2018) Achim Mall et al.   SCIENCE

Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway

(2018) Hong Yu et al.   Nature Communications

Directed -in vitro- evolution of Precambrian and extant Rubiscos

(2018) Bernardo J. Gomez-Fernandez et al.   Scientific Reports

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Engineering the Chloroplast Genome of Oleaginous Marine Microalga Nannochloropsis oceanica

(2018) Qinhua Gan et al.   Frontiers in Plant Science

FAMEs production from Scenedesmus obliquus in autotrophic, heterotrophic and mixotrophic cultures under different nitrogen conditions

(2018) Xiao-Fei Shen et al.   Environmental Science-Water Research & Technology

Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae

(2018) Leandro Buchmann et al.   BIORESOURCE TECHNOLOGY

Design and in vitro realization of carbon-conserving photorespiration

(2018) Devin L. Trudeau et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-light selection produces a fast-growing Picochlorum celeri

(2018) Joseph C. Weissman et al.   Algal Research-Biomass Biofuels and Bioproducts

Biological carbon fixation: From natural to synthetic

(2018) Fuyu Gong et al.   Journal of CO2 Utilization

An improvedEscherichia coliscreen for Rubisco identifies a protein–protein interface that can enhance CO2-fixation kinetics

(2017) Robert H. Wilson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis

(2017) Nicky Atkinson et al.   NEW PHYTOLOGIST

A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production

(2017) Eric Poliner et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant RuBisCo assembly inE. coliwith five chloroplast chaperones including BSD2

(2017) H. Aigner et al.   SCIENCE

Extracellular Electron Uptake: Among Autotrophs and Mediated by Surfaces

(2017) Pier-Luc Tremblay et al.   TRENDS IN BIOTECHNOLOGY

A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea

(2017) Takunari Kono et al.   Nature Communications

Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase

(2017) Li Wei et al.   Algal Research-Biomass Biofuels and Bioproducts

A fast and reliable strategy to generate TALEN-mediated gene knockouts in the diatom Phaeodactylum tricornutum

(2017) M. Serif et al.   Algal Research-Biomass Biofuels and Bioproducts

Recent advances in CO2uptake and fixation mechanism of cyanobacteria and microalgae

(2016) Shailendra Kumar Singh et al.   CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY

Towards industrial products from microalgae

(2016) Jesús Ruiz et al.   Energy & Environmental Science

Functional metagenomic selection of ribulose 1, 5-bisphosphate carboxylase/oxygenase from uncultivated bacteria

(2016) Vanessa A. Varaljay et al.   ENVIRONMENTAL MICROBIOLOGY

RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha

(2016) Sriram Satagopan et al.   FEBS Journal

Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity

(2016) Won-Sub Shin et al.   JOURNAL OF APPLIED PHYCOLOGY

Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803

(2016) Feiyan Liang et al.   METABOLIC ENGINEERING

Extracellular electron transfer mechanisms between microorganisms and minerals

(2016) Liang Shi et al.   NATURE REVIEWS MICROBIOLOGY

Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage

(2016) Alexander V. Ruban   PLANT PHYSIOLOGY

Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II

(2016) M.-Y. Ho et al.   SCIENCE

Water splitting–biosynthetic system with CO2reduction efficiencies exceeding photosynthesis

(2016) Chong Liu et al.   SCIENCE

A synthetic pathway for the fixation of carbon dioxide in vitro

(2016) Thomas Schwander et al.   SCIENCE

Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina

(2016) Tracey A. Beacham et al.   Algal Research-Biomass Biofuels and Bioproducts

Overexpression of Calvin cycle enzyme fructose 1,6-bisphosphatase in Chlamydomonas reinhardtii has a detrimental effect on growth

(2016) Wipawee Dejtisakdi et al.   Algal Research-Biomass Biofuels and Bioproducts

Growth of Chlorella sorokiniana on a mixture of volatile fatty acids: The effects of light and temperature

(2015) V. Turon et al.   BIORESOURCE TECHNOLOGY

Neutral red-mediated microbial electrosynthesis by Escherichia coli , Klebsiella pneumoniae , and Zymomonas mobilis

(2015) Timothy D. Harrington et al.   BIORESOURCE TECHNOLOGY

Enhancement of photosynthetic capacity in Euglena gracilis by expression of cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase leads to increases in biomass and wax ester production

(2015) Takahisa Ogawa et al.   Biotechnology for Biofuels

Small subunit of a cold-resistant plant, timothy, does not significantly alter the catalytic properties of Rubisco in transgenic rice

(2015) Hiroshi Fukayama et al.   PHOTOSYNTHESIS RESEARCH

The CO2 concentrating mechanism and photosynthetic carbon assimilation in limiting CO2 : how Chlamydomonas works against the gradient

(2015) Yingjun Wang et al.   PLANT JOURNAL

Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green algaChlamydomonas reinhardtii

(2015) Takashi Yamano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Redesigning photosynthesis to sustainably meet global food and bioenergy demand

(2015) Donald R. Ort et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

(2015) Javier A. Gimpel et al.   ACS Synthetic Biology

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Engineered Photosystem II Reaction Centers Optimize Photochemistry versus Photoprotection at Different Solar Intensities

(2014) David J. Vinyard et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803

(2014) Stefania Viola et al.   Microbial Cell Factories

TALE activation of endogenous genes in Chlamydomonas reinhardtii

(2014) Han Gao et al.   Algal Research-Biomass Biofuels and Bioproducts

High-value products from microalgae—their development and commercialisation

(2013) Michael A. Borowitzka   JOURNAL OF APPLIED PHYCOLOGY

Expression of the sub-pathways of the Chloroflexus aurantiacus 3-hydroxypropionate carbon fixation bicycle in E. coli: Toward horizontal transfer of autotrophic growth

(2013) Matthew d. Mattozzi et al.   METABOLIC ENGINEERING

A reverse glyoxylate shunt to build a non-native route from C4 to C2 in Escherichia coli

(2013) Samuel E. Mainguet et al.   METABOLIC ENGINEERING

Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide

(2013) M. W. Keller et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Potential of proton-pumping rhodopsins: engineering photosystems into microorganisms

(2013) Nico J. Claassens et al.   TRENDS IN BIOTECHNOLOGY

Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation

(2012) Benjamas Cheirsilp et al.   BIORESOURCE TECHNOLOGY

On rate limitations of electron transfer in the photosynthetic cytochrome b6f complex

(2012) S. Saif Hasan et al.   PHYSICAL CHEMISTRY CHEMICAL PHYSICS

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

(2012) A. N.-S. Mak et al.   SCIENCE

Integrated Electromicrobial Conversion of CO2 to Higher Alcohols

(2012) H. Li et al.   SCIENCE

Transgenic Introduction of a Glycolate Oxidative Cycle into A. thaliana Chloroplasts Leads to Growth Improvement

(2012) Alexandra Maier et al.   Frontiers in Plant Science

Electrosynthesis of Organic Compounds from Carbon Dioxide Is Catalyzed by a Diversity of Acetogenic Microorganisms

(2011) Kelly P. Nevin et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ecological Aspects of the Distribution of Different Autotrophic CO2Fixation Pathways

(2011) Ivan A. Berg   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Horizontal or vertical photobioreactors? How to improve microalgae photosynthetic efficiency

(2011) María Cuaresma et al.   BIORESOURCE TECHNOLOGY

Comparing Photosynthetic and Photovoltaic Efficiencies and Recognizing the Potential for Improvement

(2011) R. E. Blankenship et al.   SCIENCE

Functional Hybrid Rubisco Enzymes with Plant Small Subunits and Algal Large Subunits

(2010) Todor Genkov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Optimizing Antenna Size to Maximize Photosynthetic Efficiency

(2010) D. R. Ort et al.   PLANT PHYSIOLOGY

Design and analysis of synthetic carbon fixation pathways

(2010) A. Bar-Even et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Photorespiration: players, partners and origin

(2010) Hermann Bauwe et al.   TRENDS IN PLANT SCIENCE

Biodiesel production by microalgal biotechnology

(2009) GuanHua Huang et al.   APPLIED ENERGY

Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions

(2009) Yanna Liang et al.   BIOTECHNOLOGY LETTERS

Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle

(2008) Murray Ronald Badger et al.   JOURNAL OF EXPERIMENTAL BOTANY

Quantum Mechanics/Molecular Mechanics Study of the Catalytic Cycle of Water Splitting in Photosystem II

(2008) Eduardo M. Sproviero et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis

(2008) H. Huber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants

(2008) M. Eisenhut et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE