Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

The influence of mixed salts on the capacity of HIC adsorbers: A predictive correlation to the surface tension and the aggregation temperature

(2016) Kai Baumgartner et al.   BIOTECHNOLOGY PROGRESS

High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli

(2016) Christopher Ladd Effio et al.   JOURNAL OF BIOTECHNOLOGY

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles

(2016) Christopher Ladd Effio et al.   JOURNAL OF CHROMATOGRAPHY A

High-cell-density cultivation and recombinant protein production with Komagataella pastoris in stirred-tank bioreactors from milliliter to cubic meter scale

(2016) Andreas Schmideder et al.   PROCESS BIOCHEMISTRY

High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development

(2015) Pascal Baumann et al.   BIOTECHNOLOGY AND BIOENGINEERING

Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues

(2015) Nina Brestrich et al.   BIOTECHNOLOGY AND BIOENGINEERING

Squeeze flow rheometry as a novel tool for the characterization of highly concentrated protein solutions

(2015) Marie-Therese Schermeyer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improving Cell-Free Protein Synthesis through Genome Engineering ofEscherichia coliLacking Release Factor 1

(2015) Seok Hoon Hong et al.   CHEMBIOCHEM

Predicting recombinant protein expression experiments using molecular dynamics simulation

(2015) Andrea Schaller et al.   CHEMICAL ENGINEERING SCIENCE

Microscale and miniscale fermentation and screening

(2015) Clemens Lattermann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Deconvolution of high-throughput multicomponent isotherms using multivariate data analysis of protein spectra

(2015) Pascal Baumann et al.   ENGINEERING IN LIFE SCIENCES

Rapid automated detergent screening for the solubilization and purification of membrane proteins and complexes

(2015) Violaine Lantez et al.   ENGINEERING IN LIFE SCIENCES

Systematic purification of salt-intolerant proteins by ion-exchange chromatography: The example of human α-galactosidase A

(2015) Pascal Baumann et al.   ENGINEERING IN LIFE SCIENCES

UV absorption-based inverse modeling of protein chromatography

(2015) Tobias Hahn et al.   ENGINEERING IN LIFE SCIENCES

High throughput development of a non protein A monoclonal antibody purification process using mini-columns and bio-layer interferometry

(2015) Virginie Brenac Brochier et al.   ENGINEERING IN LIFE SCIENCES

Determination of protein phase diagrams by microbatch experiments: Exploring the influence of precipitants and pH

(2015) Kai Baumgartner et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

Integrated development of up- and downstream processes supported by the Cherry-Tag™ for real-time tracking of stability and solubility of proteins

(2015) Pascal Baumann et al.   JOURNAL OF BIOTECHNOLOGY

Simulating and Optimizing Preparative Protein Chromatography with ChromX

(2015) Tobias Hahn et al.   JOURNAL OF CHEMICAL EDUCATION

Influence of binding pH and protein solubility on the dynamic binding capacity in hydrophobic interaction chromatography

(2015) Pascal Baumann et al.   JOURNAL OF CHROMATOGRAPHY A

Downstream processing of virus-like particles: Single-stage and multi-stage aqueous two-phase extraction

(2015) Christopher Ladd Effio et al.   JOURNAL OF CHROMATOGRAPHY A

Cardiac differentiation of human pluripotent stem cells in scalable suspension culture

(2015) Henning Kempf et al.   Nature Protocols

Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp

(2014) Supattra Treerattrakool et al.   AQUACULTURE

A tool for selective inline quantification of co-eluting proteins in chromatography using spectral analysis and partial least squares regression

(2014) Nina Brestrich et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput miniaturized bioreactors for cell culture process development: Reproducibility, scalability, and control

(2014) Shahid Rameez et al.   BIOTECHNOLOGY PROGRESS

Moving through three-dimensional phase diagrams of monoclonal antibodies

(2014) Natalie Rakel et al.   BIOTECHNOLOGY PROGRESS

A model based approach for identifying robust operating conditions for industrial chromatography with process variability

(2014) Edward J. Close et al.   CHEMICAL ENGINEERING SCIENCE

A new ultrasonic high-throughput instrument for rapid DNA release from microorganisms

(2014) Marisa Hohnadel et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Three-dimensional, sharp-tipped electrodes concentrate applied fields to enable direct electrical release of intact biomarkers from cells

(2014) Mahla Poudineh et al.   LAB ON A CHIP

Cation-exchange chromatography of monoclonal antibodies: Characterisation of a novel stationary phase designed for production-scale purification

(2014) Marina Urmann et al.   mAbs

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography

(2014) Thiemo C. Huuk et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Purifying biopharmaceuticals: knowledge-based chromatographic process development

(2014) Alexander T. Hanke et al.   TRENDS IN BIOTECHNOLOGY

High throughput process development in biomanufacturing

(2014) Karol M Łącki   Current Opinion in Chemical Engineering

Mining for hemicellulases in the fungus-growing termite Pseudacanthotermes militaris using functional metagenomics

(2013) Géraldine Bastien et al.   Biotechnology for Biofuels

Quantification of protein mixture in chromatographic separation using multi-wavelength UV spectra

(2013) Mark-Henry Kamga et al.   BIOTECHNOLOGY PROGRESS

Modelling of industrial biopharmaceutical multicomponent chromatography

(2013) Edward J. Close et al.   CHEMICAL ENGINEERING RESEARCH & DESIGN

Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning

(2013) Florian Dismer et al.   CHEMICAL ENGINEERING SCIENCE

Simulated Moving Bed Chromatography: Separation and Recovery of Sugars and Ionic Liquid from Biomass Hydrolysates

(2013) Benjamin R. Caes et al.   ChemSusChem

Fast and accurate parameter sensitivities for the general rate model of column liquid chromatography

(2013) Andreas Püttmann et al.   COMPUTERS & CHEMICAL ENGINEERING

Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway

(2013) Zhen-Yu Yang et al.   CYTOTECHNOLOGY

Analytical characterization of complex, biotechnological feedstocks by pH gradient ion exchange chromatography for purification process development

(2013) Frieder Kröner et al.   JOURNAL OF CHROMATOGRAPHY A

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography

(2013) Frieder Kröner et al.   JOURNAL OF CHROMATOGRAPHY A

High throughput process development (HTPD) platform for membrane chromatography

(2013) S. Muthukumar et al.   JOURNAL OF MEMBRANE SCIENCE

Mixed electrolytes in hydrophobic interaction chromatography†

(2013) Egbert Müller et al.   JOURNAL OF SEPARATION SCIENCE

Large-Scale Functional Purification of Recombinant HIV-1 Capsid

(2013) Magdeleine Hung et al.   PLoS One

Comparison of DNA Extraction Methods in Analysis of Salivary Bacterial Communities

(2013) Vladimir Lazarevic et al.   PLoS One

Editorial: High-throughput process development

(2012) Jürgen Hubbuch   Biotechnology Journal

High-throughput methods for miniaturization and automation of monoclonal antibody purification processes

(2012) Katrin Treier et al.   BIOTECHNOLOGY PROGRESS

Hochparallele Bioprozessentwicklung in geschüttelten Mikrobioreaktoren

(2012) Pamela Wenk et al.   CHEMIE INGENIEUR TECHNIK

Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors

(2012) Wei-Ting Hsu et al.   CYTOTECHNOLOGY

Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein

(2012) Kirill Tsirulnikov et al.   FEBS LETTERS

A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture

(2012) E. Ratcliffe et al.   JOURNAL OF BIOTECHNOLOGY

Optimizing a chromatographic three component separation: A comparison of mechanistic and empiric modeling approaches

(2012) A. Osberghaus et al.   JOURNAL OF CHROMATOGRAPHY A

Experimental design in chromatography: A tutorial review

(2012) D. Brynn Hibbert   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Development and characterization of an automated high throughput screening method for optimization of protein refolding processes

(2012) Annette Berg et al.   JOURNAL OF SEPARATION SCIENCE

Molecular docking, molecular dynamics simulation, and QSAR model on potent thiazolidine-4-carboxylic acid inhibitors of influenza neuraminidase

(2012) M. Asadollahi-Baboli et al.   MEDICINAL CHEMISTRY RESEARCH

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

(2012) Peter Rohe et al.   Microbial Cell Factories

Expression, purification and crystallization of the Cmi immunity protein fromEscherichia coli

(2011) Christin Römer et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis

(2011) Peter Riedlberger et al.   BIORESOURCE TECHNOLOGY

A label-free methodology for selective protein quantification by means of absorption measurements

(2011) Sigrid K. Hansen et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optimisation of bacteriocin production of Lactococcus lactis subsp. lactis MA23, a strain isolated from Boza

(2011) NEFISE AKKOÇ et al.   INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY

Separation science is the key to successful biopharmaceuticals

(2011) Georges Guiochon et al.   JOURNAL OF CHROMATOGRAPHY A

Effects of resin ligand density on yield and impurity clearance in preparative cation exchange chromatography. II. Process characterization

(2011) Jace Fogle et al.   JOURNAL OF CHROMATOGRAPHY A

Designing new monoclonal antibody purification processes using mixed-mode chromatography sorbents

(2011) Magali Toueille et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale

(2011) Yuguo Zang et al.   PLoS One

High-throughput process development for biopharmaceutical drug substances

(2011) Rahul Bhambure et al.   TRENDS IN BIOTECHNOLOGY

Overexpression of alpha-ketoglutarate dehydrogenase in Yarrowia lipolytica and its effect on production of organic acids

(2010) Martina Holz et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Microfluidic biolector-microfluidic bioprocess control in microtiter plates

(2010) Matthias Funke et al.   BIOTECHNOLOGY AND BIOENGINEERING

Application of an aqueous two-phase systems high-throughput screening method to evaluate mAb HCP separation

(2010) Stefan A. Oelmeier et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry

(2010) Haruhito Tsutsui et al.   CLINICA CHIMICA ACTA

New method for selection of hydrogen peroxide adapted bifidobacteria cells using continuous culture and immobilized cell technology

(2010) Valeria Mozzetti et al.   Microbial Cell Factories

A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions

(2010) Vincent Dechavanne et al.   PROTEIN EXPRESSION AND PURIFICATION

Recent advances in large-scale production of monoclonal antibodies and related proteins

(2010) Abhinav A. Shukla et al.   TRENDS IN BIOTECHNOLOGY

Refolding of the human dihydrolipoamide dehydrogenase

(2009) Attila Ambrus et al.   BIOCHEMICAL ENGINEERING JOURNAL

Hydrophobic interaction chromatography in dual salt system increases protein binding capacity

(2009) Anna M. Senczuk et al.   BIOTECHNOLOGY AND BIOENGINEERING

The baffled microtiter plate: Increased oxygen transfer and improved online monitoring in small scale fermentations

(2009) Matthias Funke et al.   BIOTECHNOLOGY AND BIOENGINEERING

Insights into the modeling of adsorption isotherm systems

(2009) K.Y. Foo et al.   CHEMICAL ENGINEERING JOURNAL

A novel method to evaluate protein solubility using a high throughput screening approach

(2009) Matthias Wiendahl et al.   CHEMICAL ENGINEERING SCIENCE

Cutinase-like proteins of Mycobacterium tuberculosis: characterization of their variable enzymatic functions and active site identification

(2009) Nicholas P. West et al.   FASEB JOURNAL

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

(2009) Robert Huber et al.   Microbial Cell Factories

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

(2009) Frank Kensy et al.   Microbial Cell Factories

Rational and systematic protein purification process development: the next generation

(2009) Beckley K. Nfor et al.   TRENDS IN BIOTECHNOLOGY

Quantification of power consumption and oxygen transfer characteristics of a stirred miniature bioreactor for predictive fermentation scale-up

(2008) N.K. Gill et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening of chromatographic separations: II. Hydrophobic interaction

(2008) Jack F. Kramarczyk et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening of chromatographic separations: IV. Ion-exchange

(2008) Brian D. Kelley et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening of chromatographic separations: I. Method development and column modeling

(2008) Jonathan L. Coffman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Application of Multivariate Data Analysis for Identification and Successful Resolution of a Root Cause for a Bioprocessing Application

(2008) A.O. Kirdar et al.   BIOTECHNOLOGY PROGRESS

High-Throughput Process Development: Determination of Dynamic Binding Capacity Using Microtiter Filter Plates Filled with Chromatography Resin

(2008) T. Bergander et al.   BIOTECHNOLOGY PROGRESS

Bioprocess optimization using design-of-experiments methodology

(2008) Carl-Fredrik Mandenius et al.   BIOTECHNOLOGY PROGRESS

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

(2008) M. Wiendahl et al.   CHEMICAL ENGINEERING & TECHNOLOGY

Ibuprofen Racemate Separation by Simulated Moving Bed

(2008) Joon Sup Park et al.   JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

High-speed solubility screening assay using ultra-performance liquid chromatography/mass spectrometry in drug discovery

(2008) Taro Yamashita et al.   JOURNAL OF CHROMATOGRAPHY A

Predicting protein retention time in ion-exchange chromatography based on three-dimensional protein characterization

(2008) Li Xu et al.   JOURNAL OF CHROMATOGRAPHY A

Response surface methodology study of laccase production in Panus tigrinus liquid cultures

(2007) Daniele Quaratino et al.   BIOCHEMICAL ENGINEERING JOURNAL

Design strategies for integrated protein purification processes: challenges, progress and outlook

(2007) Beckley K Nfor et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations

(2007) Tangir Ahamed et al.   JOURNAL OF CHROMATOGRAPHY A

Fast purification process optimization using mixed-mode chromatography sorbents in pre-packed mini-columns

(2007) Virginie Brenac Brochier et al.   JOURNAL OF CHROMATOGRAPHY A

Anion-exchange membrane chromatography for purification of rotavirus-like particles

(2007) Tiago Vicente et al.   JOURNAL OF MEMBRANE SCIENCE

High-throughput automated refolding screening of inclusion bodies

(2004) Renaud Vincentelli et al.   PROTEIN SCIENCE