Journal
BIORESOURCE TECHNOLOGY
Volume 106, Issue -, Pages 138-146Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.12.019
Keywords
Biomass; Enzymatic hydrolysis; Mixing characteristics; Stirred-tank bioreactor; High-throughput bioreaction system
Funding
- TUM Graduate School
- Federal Ministry of Education and Research, Germany [0315179]
- Sud-Chemie AG, Germany
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Many factors strongly influence the enzymatic hydrolysis of biomass to fermentable sugars (feedstock composition, pretreatment, enzymes and enzyme loading). In order to optimize the reaction conditions for the hydrolysis of biomass, an accurate high-throughput bioprocess development tool is mandatory, which enables a parallelization and an easy scale-up. New S-shaped impellers were developed for magnetically inductive driven stirred-tank bioreactors at a 10 mL-scale. An efficient and reproducible homogenization was shown at 20% w/w solids loading of microcrystalline cellulose and at, 4-10% with wheat straw in 48 parallel operated stirred-tank bioreactors. The scale-up was successfully validated for the enzymatic hydrolysis of wheat straw suspensions and microcrystalline cellulose mixtures by application of a cellulase complex at a milliliter- and liter-scale. As an example, the parallel stirred-tank bioreactor system was applied for the evaluation of enzymatic batch hydrolyses of plant materials with varying pretreatments. (C) 2011 Elsevier Ltd. All rights reserved.
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