Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice

Using optogenetics to tackle systems-level questions of multicellular morphogenesis

(2020) Jonas Hartmann et al.   CURRENT OPINION IN CELL BIOLOGY

Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes

(2019) Christopher D. Reinkemeier et al.   SCIENCE

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

(2019) Lindsay B. Case et al.   SCIENCE

Light-based control of metabolic flux through assembly of synthetic organelles

(2019) Evan M. Zhao et al.   Nature Chemical Biology

Principles and applications of optogenetics in developmental biology

(2019) Daniel Krueger et al.   DEVELOPMENT

The liquid nucleome – phase transitions in the nucleus at a glance

(2019) Amy R. Strom et al.   JOURNAL OF CELL SCIENCE

Synthetic Biology Makes Polymer Materials Count

(2018) Hannes M. Beyer et al.   ADVANCED MATERIALS

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

Phase Transitions in the Assembly and Function of Human miRISC

(2018) Jessica Sheu-Gruttadauria et al.   CELL

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

Coactivator condensation at super-enhancers links phase separation and gene control

(2018) Benjamin R. Sabari et al.   SCIENCE

Mediator and RNA polymerase II clusters associate in transcription-dependent condensates

(2018) Won-Ki Cho et al.   SCIENCE

OptoBase: A Web Platform for Molecular Optogenetics

(2018) Katja Kolar et al.   ACS Synthetic Biology

A New Lens for RNA Localization: Liquid-Liquid Phase Separation

(2018) Erin M. Langdon et al.   Annual Review of Microbiology

RNA polymerase II clustering through carboxy-terminal domain phase separation

(2018) Marc Boehning et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

(2018) Benjamin S. Schuster et al.   Nature Communications

Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Intrinsically disordered sequences enable modulation of protein phase separation through distributed tyrosine motifs

(2017) Yuan Lin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Real-time observation of light-controlled transcription in living cells

(2017) Anne Rademacher et al.   JOURNAL OF CELL SCIENCE

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

A bacterial phytochrome-based optogenetic system controllable with near-infrared light

(2016) Andrii A Kaberniuk et al.   NATURE METHODS

Phase separation of signaling molecules promotes T cell receptor signal transduction

(2016) Xiaolei Su et al.   SCIENCE

Transport Selectivity of Nuclear Pores, Phase Separation, and Membraneless Organelles

(2016) H. Broder Schmidt et al.   TRENDS IN BIOCHEMICAL SCIENCES

Data2Dynamics: a modeling environment tailored to parameter estimation in dynamical systems: Fig. 1.

(2015) A. Raue et al.   BIOINFORMATICS

The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells

(2015) Daphne L. Che et al.   ACS Synthetic Biology

AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

(2015) Hannes M. Beyer et al.   PLoS One

Orthogonal Optogenetic Triple-Gene Control in Mammalian Cells

(2014) Konrad Müller et al.   ACS Synthetic Biology

A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

(2013) Konrad Müller et al.   NUCLEIC ACIDS RESEARCH

Multi-chromatic control of mammalian gene expression and signaling

(2013) Konrad Müller et al.   NUCLEIC ACIDS RESEARCH

Real-Time Dynamics of RNA Polymerase II Clustering in Live Human Cells

(2013) I. I. Cisse et al.   SCIENCE

Likelihood based observability analysis and confidence intervals for predictions of dynamic models

(2012) Clemens Kreutz et al.   BMC Systems Biology

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

A Synthetic Optogenetic Transcription Device Enhances Blood-Glucose Homeostasis in Mice

(2011) H. Ye et al.   SCIENCE

Structural and practical identifiability analysis of partially observed dynamical models by exploiting the profile likelihood

(2009) A. Raue et al.   BIOINFORMATICS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE