Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq
Published 2021 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Identifying genome-wide off-target sites of CRISPR RNA–guided nucleases and deaminases with Digenome-seq
Authors
Keywords
-
Journal
Nature Protocols
Volume -, Issue -, Pages -
Publisher
Springer Science and Business Media LLC
Online
2021-01-19
DOI
10.1038/s41596-020-00453-6
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Genome-wide profiling of adenine base editor specificity by EndoV-seq
- (2019) Puping Liang et al. Nature Communications
- Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
- (2019) Benjamin P. Kleinstiver et al. NATURE BIOTECHNOLOGY
- Genome-wide target specificity of CRISPR RNA-guided adenine base editors
- (2019) Daesik Kim et al. NATURE BIOTECHNOLOGY
- Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases
- (2019) Daesik Kim et al. Annual Review of Biochemistry
- Base editing with a Cpf1–cytidine deaminase fusion
- (2018) Xiaosa Li et al. NATURE BIOTECHNOLOGY
- Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion
- (2018) Xiao Wang et al. NATURE BIOTECHNOLOGY
- An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities
- (2018) Jason M Gehrke et al. NATURE BIOTECHNOLOGY
- DIG-seq: a genome-wide CRISPR off-target profiling method using chromatin DNA
- (2018) Daesik Kim et al. GENOME RESEARCH
- Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
- (2017) Nicole M. Gaudelli et al. NATURE
- Genome-wide target specificities of CRISPR RNA-guided programmable deaminases
- (2017) Daesik Kim et al. NATURE BIOTECHNOLOGY
- Digenome-seq web tool for profiling CRISPR specificity
- (2017) Jeongbin Park et al. NATURE METHODS
- Mapping the genomic landscape of CRISPR–Cas9 cleavage
- (2017) Peter Cameron et al. NATURE METHODS
- CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
- (2017) Shengdar Q Tsai et al. NATURE METHODS
- In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni
- (2017) Eunji Kim et al. Nature Communications
- Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery
- (2017) Holly A. Rees et al. Nature Communications
- BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks
- (2017) Winston X. Yan et al. Nature Communications
- Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
- (2017) David W. Morgens et al. Nature Communications
- Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
- (2016) Daesik Kim et al. GENOME RESEARCH
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
- (2016) Alexis C. Komor et al. NATURE
- Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells
- (2016) Daesik Kim et al. NATURE BIOTECHNOLOGY
- Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
- (2016) Shengdar Q. Tsai et al. NATURE REVIEWS GENETICS
- Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
- (2016) K. Nishida et al. SCIENCE
- Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
- (2015) Bernd Zetsche et al. CELL
- Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9
- (2015) Taeyoung Koo et al. MOLECULES AND CELLS
- In vivo genome editing using Staphylococcus aureus Cas9
- (2015) F. Ann Ran et al. NATURE
- Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
- (2015) Xiaoling Wang et al. NATURE BIOTECHNOLOGY
- Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
- (2015) Daesik Kim et al. NATURE METHODS
- Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
- (2015) Ritambhara Singh et al. NUCLEIC ACIDS RESEARCH
- CasOT: a genome-wide Cas9/gRNA off-target searching tool
- (2014) A. Xiao et al. BIOINFORMATICS
- Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
- (2014) S. Bae et al. BIOINFORMATICS
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
- (2014) Richard L Frock et al. NATURE BIOTECHNOLOGY
- A guide to genome engineering with programmable nucleases
- (2014) Hyongbum Kim et al. NATURE REVIEWS GENETICS
- CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
- (2014) Tessa G. Montague et al. NUCLEIC ACIDS RESEARCH
- Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
- (2013) S. W. Cho et al. GENOME RESEARCH
- DNA targeting specificity of RNA-guided Cas9 nucleases
- (2013) Patrick D Hsu et al. NATURE BIOTECHNOLOGY
- Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
- (2013) Seung Woo Cho et al. NATURE BIOTECHNOLOGY
- High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
- (2013) Vikram Pattanayak et al. NATURE BIOTECHNOLOGY
- High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- (2013) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
- (2013) Thomas J. Cradick et al. NUCLEIC ACIDS RESEARCH
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- (2013) L. Cong et al. SCIENCE
- RNA-programmed genome editing in human cells
- (2013) Martin Jinek et al. eLife
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases
- (2011) H. J. Lee et al. GENOME RESEARCH
- An unbiased genome-wide analysis of zinc-finger nuclease specificity
- (2011) Richard Gabriel et al. NATURE BIOTECHNOLOGY
- Integrative genomics viewer
- (2011) James T Robinson et al. NATURE BIOTECHNOLOGY
- Targeted chromosomal deletions in human cells using zinc finger nucleases
- (2009) H. J. Lee et al. GENOME RESEARCH
- Chromosomal translocations induced at specified loci in human stem cells
- (2009) E. Brunet et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Analyzing real-time PCR data by the comparative CT method
- (2008) Thomas D Schmittgen et al. Nature Protocols
Discover Peeref hubs
Discuss science. Find collaborators. Network.
Join a conversationBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started