Combining protein and metabolic engineering to construct efficient microbial cell factories

Protein Engineering for Improving and Diversifying Natural Product Biosynthesis

(2020) Chenyi Li et al.   TRENDS IN BIOTECHNOLOGY

High-Throughput Screening Technology in Industrial Biotechnology

(2020) Weizhu Zeng et al.   TRENDS IN BIOTECHNOLOGY

Systems Metabolic Engineering Strategies: Integrating Systems and Synthetic Biology with Metabolic Engineering

(2019) Kyeong Rok Choi et al.   TRENDS IN BIOTECHNOLOGY

Enzyme annotation for orphan and novel reactions using knowledge of substrate reactive sites

(2019) Noushin Hadadi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vivo Protein Evolution, Next Generation Protein Engineering Strategy: from Random Approach to Target-specific Approach

(2019) Jin Young Kim et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Activity-stability trade-off in random mutant proteins

(2019) Ryo Kurahashi et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Machine learning-assisted directed protein evolution with combinatorial libraries

(2019) Zachary Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo protein design by citizen scientists

(2019) Brian Koepnick et al.   NATURE

Synthetic evolution

(2019) Anna J. Simon et al.   NATURE BIOTECHNOLOGY

Machine-learning-guided directed evolution for protein engineering

(2019) Kevin K. Yang et al.   NATURE METHODS

Advances in protein structure prediction and design

(2019) Brian Kuhlman et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

From directed evolution to computational enzyme engineering—A review

(2019) Ratul Chowdhury et al.   AICHE JOURNAL

Recent trends in metabolic engineering of microbial chemical factories

(2019) Yi Liu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Dynamic control of toxic natural product biosynthesis by an artificial regulatory circuit

(2019) Chaoning Liang et al.   METABOLIC ENGINEERING

Exploiting the synthetic potential of sesquiterpene cyclases for generating unnatural terpenoids

(2018) Andreas Kirschning et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

(2018) Ioannis Mougiakos et al.   CURRENT OPINION IN BIOTECHNOLOGY

Molecular dynamics study of taxadiene synthase catalysis

(2018) Andrés M. Escorcia et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

A Processive Protein Chimera Introduces Mutations across Defined DNA Regions In Vivo

(2018) Christopher L. Moore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Iterative genome editing of Escherichia coli for 3-hydroxypropionic acid production

(2018) Rongming Liu et al.   METABOLIC ENGINEERING

Multiplexed precision genome editing with trackable genomic barcodes in yeast

(2018) Kevin R Roy et al.   NATURE BIOTECHNOLOGY

Burden-driven feedback control of gene expression

(2018) Francesca Ceroni et al.   NATURE METHODS

Directed evolution of multiple genomic loci allows the prediction of antibiotic resistance

(2018) Ákos Nyerges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Advances in Industrial Biotechnology Using CRISPR-Cas Systems

(2018) Paul D. Donohoue et al.   TRENDS IN BIOTECHNOLOGY

Precise control of SCRaMbLE in synthetic haploid and diploid yeast

(2018) Bin Jia et al.   Nature Communications

CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window

(2018) Shakked O. Halperin et al.   NATURE

Current trends in protein engineering: updates and progress

(2018) Rajeshwari Sinha et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

(2018) Codruta Ignea et al.   Nature Chemical Biology

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

(2018) Haoran Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Two-step pathway for isoprenoid synthesis

(2018) Alkiviadis Orfefs Chatzivasileiou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

(2018) Satoshi Yuzawa et al.   Nature Communications

Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme

(2017) Yu Zhuang et al.   METABOLIC ENGINEERING

Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins

(2017) Claire N. Bedbrook et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering fungal de novo fatty acid synthesis for short chain fatty acid production

(2017) Jan Gajewski et al.   Nature Communications

Combinatorial pathway optimization inEscherichia coliby directed co-evolution of rate-limiting enzymes and modular pathway engineering

(2016) Xiaomei Lv et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein engineering approaches to chemical biotechnology

(2016) Zhen Chen et al.   CURRENT OPINION IN BIOTECHNOLOGY

The coming of age of de novo protein design

(2016) Po-Ssu Huang et al.   NATURE

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

In vivo continuous evolution of genes and pathways in yeast

(2016) Nathan Crook et al.   Nature Communications

Prospects of microbial cell factories developed through systems metabolic engineering

(2016) Martin Gustavsson et al.   Microbial Biotechnology

Transcription factor-based biosensors in biotechnology: current state and future prospects

(2015) Regina Mahr et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications

(2015) Yuvaraj Ravikumar et al.   Biotechnology Journal

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

(2015) Wei Chen et al.   METABOLIC ENGINEERING

Computational protein design enables a novel one-carbon assimilation pathway

(2015) Justin B. Siegel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Incorporating unnatural amino acids to engineer biocatalysts for industrial bioprocess applications

(2015) Yuvaraj Ravikumar et al.   Biotechnology Journal

Computational tools for designing and engineering enzymes

(2014) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Integrating the Protein and Metabolic Engineering Toolkits for Next-Generation Chemical Biosynthesis

(2013) Christopher M. Pirie et al.   ACS Chemical Biology

Deregulation of Feedback Inhibition of Phosphoenolpyruvate Carboxylase for Improved Lysine Production in Corynebacterium glutamicum

(2013) Zhen Chen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Rational Design of Allosteric Regulation of Homoserine Dehydrogenase by a Nonnatural Inhibitor l-Lysine

(2013) Zhen Chen et al.   ACS Synthetic Biology

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

De Novo Enzyme Design Using Rosetta3

(2011) Florian Richter et al.   PLoS One

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control

(2010) E. Leonard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE