Biological strategies for oligo/polysaccharide synthesis: biocatalyst and microbial cell factory

Development of slowly digestible starch derived alpha-glucans with 4,6-α-glucanotransferase and branching sucrase enzymes

(2020) Evelien te Poele et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Structures, physico-chemical properties, production and (potential) applications of sucrose-derived α-D-glucans synthesized by glucansucrases

(2020) Xiaodan Li et al.   CARBOHYDRATE POLYMERS

Enzymatic Synthesis of Artificial Polysaccharides

(2020) Peter J. Smith et al.   ACS Sustainable Chemistry & Engineering

Enzymatic Building‐Block Synthesis for Solid‐Phase Automated Glycan Assembly

(2020) Andrea Marchesi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

UDP-Sugar Producing Pyrophosphorylases: Distinct and Essential Enzymes With Overlapping Substrate Specificities, Providing de novo Precursors for Glycosylation Reactions

(2019) Daniel Decker et al.   Frontiers in Plant Science

Synthesis and Characterization of Sialylated Lactose- and Lactulose-Derived Oligosaccharides by Trypanosoma cruzi Trans-sialidase

(2019) Hien T. T. Pham et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Futile encounter engineering of the DSR-M dextransucrase modifies the resulting polymer length

(2019) Marion Claverie et al.   BIOCHEMISTRY

Production of 3‐Fucosyllactose in Engineered Escherichia coli with α‐1,3‐Fucosyltransferase from Helicobacter pylori

(2019) Sang‐Min Jung et al.   Biotechnology Journal

Chemoenzymatic Synthesis of C6-Modified Sugar Nucleotides To Probe the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa

(2019) Sanaz Ahmadipour et al.   ORGANIC LETTERS

Development of Semisynthetic, Regioselective Pathways for Accessing the Missing Sulfation Patterns of Chondroitin Sulfate

(2019) Giulia Vessella et al.   BIOMACROMOLECULES

Enhanced production of 2’‐fucosyllactose from fucose by elimination of rhamnose isomerase and arabinose isomerase in engineered Escherichia coli

(2019) Sang‐Min Jung et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

(2019) Dan-Hua Meng et al.   Microbial Cell Factories

Reprogramming the enzymatic assembly line for site-specific fucosylation

(2019) Jinfeng Ye et al.   Nature Catalysis

Biosynthesis of the human milk oligosaccharide 3‐fucosyllactose in metabolically engineered Escherichia coli via the salvage pathway through increasing GTP synthesis and β‐galactosidase modification

(2019) Yun Hee Choi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Modular pathway engineering of key precursor supply pathways for lacto-N-neotetraose production in Bacillus subtilis

(2019) Xiaomin Dong et al.   Biotechnology for Biofuels

Characterization of the biosynthetic pathway of nucleotide sugar precursor UDP-glucose during sphingan WL gum production in Sphingomonas sp. WG

(2019) Hui Li et al.   JOURNAL OF BIOTECHNOLOGY

Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2

(2019) Asher Williams et al.   Microbial Cell Factories

A Study for the Access to a Semi-synthetic Regioisomer of Natural Fucosylated Chondroitin Sulfate with Fucosyl Branches on N-acetyl-Galactosamine Units

(2019) Giulia Vessella et al.   Marine Drugs

Exploring the Synthetic Application of Helicobacter pylori α1,3/4-Fucosyltransferase FucTIII toward the Syntheses of Fucosylated Human Milk Glycans and Lewis Antigens

(2019) Teng-Wei Tsai et al.   ACS Catalysis

Substrate Characterization of Bacteroides fragilis α1,3/4-Fucosyltransferase Enabling Access to Programmable One-Pot Enzymatic Synthesis of KH-1 Antigen

(2019) Hsin-Hui Huang et al.   ACS Catalysis

Chemoenzymatic Synthesis of Heparan Sulfate Mimetic Glycopolymers and Their Interactions with the Receptor for Advanced Glycation End-Product

(2019) Jun Li et al.   ACS Macro Letters

Engineering the Substrate Transport and Cofactor Regeneration Systems for Enhancing 2′-Fucosyllactose Synthesis in Bacillus subtilis

(2019) Jieying Deng et al.   ACS Synthetic Biology

Chemoenzymatic synthesis of homogeneous chondroitin polymers and its derivatives

(2019) Yaqian Wang et al.   CARBOHYDRATE POLYMERS

Chemoenzymatic Assembly of Mammalian O-Mannose Glycans

(2018) Caicai Meng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Real-time monitoring of hyaluronic acid fermentation by in situ transflectance spectroscopy

(2018) Kirubhakaran Puvendran et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis

(2018) Christian Toonstra et al.   BIOCONJUGATE CHEMISTRY

A microbial-enzymatic strategy for producing chondroitin sulfate glycosaminoglycans

(2018) Zhengxiong Zhou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biosynthesis of superparamagnetic polymer microbeads via simple precipitation of enzymatically synthesized short-chain amylose

(2018) Ke Luo et al.   CARBOHYDRATE POLYMERS

A dextran with unique rheological properties produced by the dextransucrase from Oenococcus kitaharae DSM 17330

(2018) Marlène Vuillemin et al.   CARBOHYDRATE POLYMERS

Toward Automated Enzymatic Synthesis of Oligosaccharides

(2018) Liuqing Wen et al.   CHEMICAL REVIEWS

Chemoenzymatic Synthesis of Asymmetrical Multi-Antennary N -Glycans to Dissect Glycan-Mediated Interactions between Human Sperm and Oocytes

(2018) Zoeisha S. Chinoy et al.   CHEMISTRY-A EUROPEAN JOURNAL

Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies

(2018) Jochen Schmid   CURRENT OPINION IN BIOTECHNOLOGY

Loop engineering of an α-1,3/4- l -fucosidase for improved synthesis of human milk oligosaccharides

(2018) Birgitte Zeuner et al.   ENZYME AND MICROBIAL TECHNOLOGY

Synthesis of rebaudioside D, using glycosyltransferase UGTSL2 and in situ UDP-glucose regeneration

(2018) Liangliang Chen et al.   FOOD CHEMISTRY

Enhancing fructosylated chondroitin production in Escherichia coli K4 by balancing the UDP-precursors

(2018) Quan Zhang et al.   METABOLIC ENGINEERING

Engineering of α-1,3-fucosyltransferases for production of 3-fucosyllactose in Escherichia coli

(2018) Jiwon Yu et al.   METABOLIC ENGINEERING

Production of a human milk oligosaccharide 2′-fucosyllactose by metabolically engineered Saccharomyces cerevisiae

(2018) Sora Yu et al.   Microbial Cell Factories

Substrate specificity and transfucosylation activity of GH29 α- l -fucosidases for enzymatic production of human milk oligosaccharides

(2018) Birgitte Zeuner et al.   New Biotechnology

Hyaluronic Acid in the Third Millennium

(2018) Arianna Fallacara et al.   Polymers

Facile and Stereo-Selective Synthesis of UDP-α-D-xylose and UDP-β-L-arabinose Using UDP-Sugar Pyrophosphorylase

(2018) JiaJia Wang et al.   Frontiers in Chemistry

Human Milk Oligosaccharides and Associations With Immune-Mediated Disease and Infection in Childhood: A Systematic Review

(2018) Alice M. Doherty et al.   Frontiers in Pediatrics

Enzymatic Synthesis of Human Milk Fucosides α1,2-Fucosyl para -Lacto-N -Hexaose and its Isomeric Derivatives

(2018) Jia-Lin Fang et al.   ADVANCED SYNTHESIS & CATALYSIS

Enzymatic Synthesis of 6′-Sialyllactose, a Dominant Sialylated Human Milk Oligosaccharide, by a Novel exo -α-Sialidase from Bacteroides fragilis NCTC9343

(2018) Longcheng Guo et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Construction of a novel UDP-rhamnose regeneration system by a two-enzyme reaction system and application in glycosylation of flavonoid

(2018) Jianjun Pei et al.   BIOCHEMICAL ENGINEERING JOURNAL

Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine

(2018) Reza Mahour et al.   JOURNAL OF BIOTECHNOLOGY

Synthesis of modular building blocks using glycosyl phosphate donors for the construction of asymmetric N-glycans

(2018) Supriya Dey et al.   TETRAHEDRON

Neutral Genetic Drift-Based Engineering of a Sucrose-Utilizing Enzyme toward Glycodiversification

(2018) David Daudé et al.   ACS Catalysis

Biosynthesis of a Functional Human Milk Oligosaccharide, 2′-Fucosyllactose, and l-Fucose Using Engineered Saccharomyces cerevisiae

(2018) Jing-Jing Liu et al.   ACS Synthetic Biology

α-Glucan Phosphorylase-Catalyzed Enzymatic Reactions Using Analog Substrates to Synthesize Non-Natural Oligo- and Polysaccharides

(2018) Jun-ichi Kadokawa   Catalysts

Engineering two species of yeast as cell factories for 2′-fucosyllactose

(2018) Kerry Hollands et al.   METABOLIC ENGINEERING

Immobilization-stabilization of a complex multimeric sucrose synthase from Nitrosomonas europaea. Synthesis of UDP-glucose

(2017) Alejandro H. Orrego et al.   ENZYME AND MICROBIAL TECHNOLOGY

A Chemoenzymatic Route to a Class of Sucrose Esters

(2017) Christian Possiel et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

One-pot synthesis of GDP- l -fucose by a four-enzyme cascade expressed in Lactococcus lactis

(2017) Ling Li et al.   JOURNAL OF BIOTECHNOLOGY

Production of human milk oligosaccharides by enzymatic and whole-cell microbial biotransformations

(2017) Georg A. Sprenger et al.   JOURNAL OF BIOTECHNOLOGY

Substrate Preference and Interplay of Fucosyltransferase 8 and N-Acetylglucosaminyltransferases

(2017) Tzu-Hao Tseng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoenzymatic Approach for the Preparation of Asymmetric Bi-, Tri-, and Tetra-Antennary N-Glycans from a Common Precursor

(2017) Ivan A. Gagarinov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins

(2017) Chao Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metabolic engineering of Escherichia coli for the production of 2′-fucosyllactose and 3-fucosyllactose through modular pathway enhancement

(2017) Di Huang et al.   METABOLIC ENGINEERING

Synthesis of asymmetrical multiantennary human milk oligosaccharides

(2017) Anthony R. Prudden et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Chemoenzymatic synthesis of heparan sulfate and heparin oligosaccharides and NMR analysis: paving the way to a diverse library for glycobiologists

(2017) Xing Zhang et al.   Chemical Science

Enhanced Biosynthesis of Hyaluronic Acid Using Engineered Corynebacterium glutamicum Via Metabolic Pathway Regulation

(2017) Fangyu Cheng et al.   Biotechnology Journal

Substrate Specificity and Inhibitor Sensitivity of Plant UDP-Sugar Producing Pyrophosphorylases

(2017) Daniel Decker et al.   Frontiers in Plant Science

Engineering S. equi subsp. zooepidemicus towards concurrent production of hyaluronic acid and chondroitin biopolymers of biomedical interest

(2017) Donatella Cimini et al.   AMB Express

Advances in Enzymatic Glycoside Synthesis

(2016) Phillip M. Danby et al.   ACS Chemical Biology

Bioengineered heparins and heparan sulfates

(2016) Li Fu et al.   ADVANCED DRUG DELIVERY REVIEWS

Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

(2016) Martin Lemmerer et al.   ADVANCED SYNTHESIS & CATALYSIS

Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′-Diphosphate-Glucose by Sucrose Synthase

(2016) Alexander Gutmann et al.   ADVANCED SYNTHESIS & CATALYSIS

A Kinase-Independent One-Pot Multienzyme Cascade for an Expedient Synthesis of Guanosine 5′-Diphospho-d-mannose

(2016) Martin Pfeiffer et al.   ADVANCED SYNTHESIS & CATALYSIS

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

(2016) Katharina Schmölzer et al.   BIOTECHNOLOGY ADVANCES

Solubilization and Iterative Saturation Mutagenesis of α1,3-fucosyltransferase fromHelicobacter pylorito enhance its catalytic efficiency

(2016) Yun Hee Choi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Integrated process design for biocatalytic synthesis by a Leloir Glycosyltransferase: UDP-glucose production with sucrose synthase

(2016) Katharina Schmölzer et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-titer biosynthesis of hyaluronic acid by recombinantCorynebacterium glutamicum

(2016) Fangyu Cheng et al.   Biotechnology Journal

Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass in Bacillus subtilis

(2016) Linpei Zhang et al.   BIOTECHNOLOGY LETTERS

Efficient biosynthesis of polysaccharides chondroitin and heparosan by metabolically engineered Bacillus subtilis

(2016) Peng Jin et al.   CARBOHYDRATE POLYMERS

A Modular Approach to a Library of Semi-Synthetic Fucosylated Chondroitin Sulfate Polysaccharides with Different Sulfation and Fucosylation Patterns

(2016) Antonio Laezza et al.   CHEMISTRY-A EUROPEAN JOURNAL

Divergent Chemoenzymatic Synthesis of Asymmetrical-Core-Fucosylated and Core-UnmodifiedN-Glycans

(2016) Tiehai Li et al.   CHEMISTRY-A EUROPEAN JOURNAL

Characterization of UDP-glucose dehydrogenase from Pasteurella multocida CVCC 408 and its application in hyaluronic acid biosynthesis

(2016) Xin Chu et al.   ENZYME AND MICROBIAL TECHNOLOGY

Biological roles of glycans

(2016) Ajit Varki   GLYCOBIOLOGY

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems

(2016) Barbara Petschacher et al.   JOURNAL OF BIOTECHNOLOGY

Chemoenzymatic Synthesis of a Library of Human Milk Oligosaccharides

(2016) Zhongying Xiao et al.   JOURNAL OF ORGANIC CHEMISTRY

Modular optimization of multi-gene pathways for fumarate production

(2016) Xiulai Chen et al.   METABOLIC ENGINEERING

Production of specific-molecular-weight hyaluronan by metabolically engineered Bacillus subtilis 168

(2016) Peng Jin et al.   METABOLIC ENGINEERING

Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies

(2016) Sachin S. Shivatare et al.   Nature Chemistry

Precision Synthesis of Functional Polysaccharide Materials by Phosphorylase-Catalyzed Enzymatic Reactions

(2016) Jun-ichi Kadokawa   Polymers

High-titer biosynthesis of hyaluronic acid by recombinantCorynebacterium glutamicum

(2016) Fangyu Cheng et al.   Biotechnology Journal

Novel α-L-Fucosidases from a Soil Metagenome for Production of Fucosylated Human Milk Oligosaccharides

(2016) Mateusz Lezyk et al.   PLoS One

Enzyme Module Systems for the Synthesis of Uridine 5′-Diphospho-α-D -glucuronic Acid and Non-Sulfated Human Natural Killer Cell-1 (HNK-1) Epitope

(2015) Leonie Engels et al.   ADVANCED SYNTHESIS & CATALYSIS

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

(2015) Patricia Wildberger et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enhanced production of leech hyaluronidase by optimizing secretion and cultivation in Pichia pastoris

(2015) Zhen Kang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code

(2015) Dolores Solís et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Chemical Fucosylation of a Polysaccharide: A Semisynthetic Access to Fucosylated Chondroitin Sulfate

(2015) Antonio Laezza et al.   BIOMACROMOLECULES

Glycoside phosphorylases: Structure, catalytic properties and biotechnological potential

(2015) Vladimír Puchart   BIOTECHNOLOGY ADVANCES

Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin

(2015) Jong Youn Baik et al.   Biotechnology Journal

Engineering a branch of the UDP-precursor biosynthesis pathway enhances the production of capsular polysaccharide inEscherichia coliO5:K4:H4

(2015) Donatella Cimini et al.   Biotechnology Journal

Enzymatic production of specifically distributed hyaluronan oligosaccharides

(2015) Panhong Yuan et al.   CARBOHYDRATE POLYMERS

Chemoenzymatic synthesis of α-dystroglycan core M1 O-mannose glycans

(2015) Yan Zhang et al.   CHEMICAL COMMUNICATIONS

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-d-galactoside sialyltransferase by active-site redesign

(2015) Katharina Schmölzer et al.   CHEMICAL COMMUNICATIONS

Improved one-pot multienzyme (OPME) systems for synthesizing UDP-uronic acids and glucuronides

(2015) Musleh M. Muthana et al.   CHEMICAL COMMUNICATIONS

Approaches toward High-Mannose-Type Glycan Libraries

(2015) Kohki Fujikawa et al.   CHEMICAL RECORD

Construction of a High-Mannose-Type Glycan Library by a Renewed Top-Down Chemo-Enzymatic Approach

(2015) Kohki Fujikawa et al.   CHEMISTRY-A EUROPEAN JOURNAL

Enhancing GDP-fucose production in recombinant Escherichia coli by metabolic pathway engineering

(2015) Yafei Zhai et al.   ENZYME AND MICROBIAL TECHNOLOGY

Modulating the regioselectivity of a Pasteurella multocida sialyltransferase for biocatalytic production of 3′- and 6′-sialyllactose

(2015) Yao Guo et al.   ENZYME AND MICROBIAL TECHNOLOGY

Sugar-dependent modulation of neuronal development, regeneration, and plasticity by chondroitin sulfate proteoglycans

(2015) Gregory M. Miller et al.   EXPERIMENTAL NEUROLOGY

The Crystal Structure of Nitrosomonas europaea Sucrose Synthase Reveals Critical Conformational Changes and Insights into Sucrose Metabolism in Prokaryotes

(2015) Rui Wu et al.   JOURNAL OF BACTERIOLOGY

Enhanced production of 2′-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase

(2015) Young-Wook Chin et al.   JOURNAL OF BIOTECHNOLOGY

Production of chondroitin in metabolically engineered E. coli

(2015) Wenqin He et al.   METABOLIC ENGINEERING

Efficient chemoenzymatic synthesis of an N-glycan isomer library

(2015) Lei Li et al.   Chemical Science

Optimization of bioprocess conditions improves production of a CHO cell-derived, bioengineered heparin

(2015) Jong Youn Baik et al.   Biotechnology Journal

Engineering a branch of the UDP-precursor biosynthesis pathway enhances the production of capsular polysaccharide inEscherichia coliO5:K4:H4

(2015) Donatella Cimini et al.   Biotechnology Journal

Enzymatic Redox Cascade for One-Pot Synthesis of Uridine 5′-Diphosphate Xylose from Uridine 5′-Diphosphate Glucose

(2014) Thomas Eixelsberger et al.   ADVANCED SYNTHESIS & CATALYSIS

Galactosyl-Lactose Sialylation Using Trypanosoma cruzi trans-Sialidase as the Biocatalyst and Bovine κ-Casein-Derived Glycomacropeptide as the Donor Substrate

(2014) Maarten H. Wilbrink et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The P170 expression system enhances hyaluronan molecular weight and production in metabolically-engineered Lactococcus lactis

(2014) Ashish Singh Chauhan et al.   BIOCHEMICAL ENGINEERING JOURNAL

Ratio of intracellular precursors concentration and their flux influences hyaluronic acid molecular weight in Streptococcus zooepidemicus and recombinant Lactococcus lactis

(2014) Sneh Sanjay Badle et al.   BIORESOURCE TECHNOLOGY

Production of lycopene by metabolically-engineered Escherichia coli

(2014) Tao Sun et al.   BIOTECHNOLOGY LETTERS

A one-pot approach to bio-synthesize globotriose and its derivatives from simpler substrates

(2014) Xueer Zhao et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Constructing a recombinant hyaluronic acid biosynthesis operon and producing food-grade hyaluronic acid in Lactococcus lactis

(2014) Juzheng Sheng et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

One-Pot Enzymatic Conversion of Sucrose to Synthetic Amylose by using Enzyme Cascades

(2014) Peng Qi et al.   ACS Catalysis

Top-Down Chemoenzymatic Approach to a High-Mannose-Type Glycan Library: Synthesis of a Common Precursor and Its Enzymatic Trimming

(2013) Akihiko Koizumi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Tailored Design and Synthesis of Heparan Sulfate Oligosaccharide Analogues Using Sequential One-Pot Multienzyme Systems

(2013) Yi Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Biosynthesis of nucleotide sugars by a promiscuous UDP-sugar pyrophosphorylase from Arabidopsis thaliana (AtUSP)

(2013) Jun Liu et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Metabolic engineering of Corynebacterium glutamicum to produce GDP-l-fucose from glucose and mannose

(2013) Young-Wook Chin et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

WbgL: a novel bacterial α1,2-fucosyltransferase for the synthesis of 2′-fucosyllactose

(2013) Leonie Engels et al.   GLYCOBIOLOGY

Enhanced production of l-phenylalanine in Corynebacterium glutamicum due to the introduction of Escherichia coli wild-type gene aroH

(2013) Chuanzhi Zhang et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Effective Sugar Nucleotide Regeneration for the Large-Scale Enzymatic Synthesis of Globo H and SSEA4

(2013) Tsung-I Tsai et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Construction of Escherichia coli strains with chromosomally integrated expression cassettes for the synthesis of 2′-fucosyllactose

(2013) Florian Baumgärtner et al.   Microbial Cell Factories

Enzyme catalysed production of sialylated human milk oligosaccharides and galactooligosaccharides by Trypanosoma cruzi trans-sialidase

(2013) Jesper Holck et al.   New Biotechnology

Efficient Enzymatic Synthesis of Guanosine 5′-Diphosphate-Sugars and Derivatives

(2013) Lei Li et al.   ORGANIC LETTERS

A General Strategy for the Chemoenzymatic Synthesis of Asymmetrically Branched N-Glycans

(2013) Z. Wang et al.   SCIENCE

A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Directly Sialylating Lewisx

(2012) Go Sugiarto et al.   ACS Chemical Biology

Synthesis of β(1,3) oligoglucans exhibiting a Dectin-1 binding affinity and their biological evaluation

(2012) Hiroshi Tanaka et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4 towards galactose, glucose, and their derivatives

(2012) Yang Zou et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP)

(2012) Musleh M. Muthana et al.   CHEMICAL COMMUNICATIONS

Identification of Amino Acid Residues Required for the Substrate Specificity of Human and Mouse Chondroitin Sulfate Hydrolase (Conventional Hyaluronidase-4)

(2012) Tomoyuki Kaneiwa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Modular Pathway Engineering of Diterpenoid Synthases and the Mevalonic Acid Pathway for Miltiradiene Production

(2012) Yongjin J. Zhou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin

(2012) Jong Youn Baik et al.   METABOLIC ENGINEERING

Glycosynthases as tools for the production of glycan analogs of natural products

(2012) Beatrice Cobucci-Ponzano et al.   NATURAL PRODUCT REPORTS

The Predominance of Type I Oligosaccharides Is a Feature Specific to Human Breast Milk

(2012) Tadasu Urashima et al.   Advances in Nutrition

A common structural blueprint for plant UDP-sugar-producing pyrophosphorylases

(2011) Leszek A. Kleczkowski et al.   BIOCHEMICAL JOURNAL

Synthesis of GDP-mannose using coupling fermentation of recombinant Escherichia coli

(2011) Jia Honghong et al.   BIOTECHNOLOGY LETTERS

Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4

(2011) Min Chen et al.   CARBOHYDRATE RESEARCH

The Chemistry and Biology of Trypanosomal trans-Sialidases: Virulence Factors in Chagas Disease and Sleeping Sickness

(2011) Roland Schauer et al.   CHEMBIOCHEM

The Structure of Sucrose Synthase-1 fromArabidopsis thalianaand Its Functional Implications

(2011) Yi Zheng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering of cellobiose phosphorylase for glycoside synthesis

(2011) Manu R.M. de Groeve et al.   JOURNAL OF BIOTECHNOLOGY

Substrate Promiscuity of N-Acetylhexosamine 1-Kinases

(2011) Yanhong Li et al.   MOLECULES

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

UDP-Sugar Pyrophosphorylase: A New Old Mechanism for Sugar Activation

(2011) L. A. Kleczkowski et al.   PLANT PHYSIOLOGY

Chemoenzymatic Synthesis of Homogeneous Ultralow Molecular Weight Heparins

(2011) Y. Xu et al.   SCIENCE

Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase

(2010) Ikuko Kakizaki et al.   FEBS Journal

Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4

(2010) Donatella Cimini et al.   JOURNAL OF BIOTECHNOLOGY

Structural Basis for the Broad Substrate Range of the UDP-Sugar Pyrophosphorylase from Leishmania major

(2010) Achim Dickmanns et al.   JOURNAL OF MOLECULAR BIOLOGY

Hyaluronic acid production is enhanced by the additional co-expression of UDP-glucose pyrophosphorylase in Lactococcus lactis

(2009) Shashi Bala Prasad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A chemoenzymatic route to N-acetylglucosamine-1-phosphate analogues: substrate specificity investigations of N-acetylhexosamine 1-kinase

(2009) Li Cai et al.   CHEMICAL COMMUNICATIONS

Mechanism for the effect of agitation on the molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus

(2009) Xu Zhang et al.   FOOD CHEMISTRY

Identification and functional characterization of the NanH extracellular sialidase from Corynebacterium diphtheriae

(2009) Seonghun Kim et al.   JOURNAL OF BIOCHEMISTRY

Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920

(2009) Shu-Jen Chen et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Phosphomannose isomerase/GDP-mannose pyrophosphorylase from Pyrococcus furiosus: a thermostable biocatalyst for the synthesis of guanidinediphosphate-activated and mannose-containing sugar nucleotides

(2009) Rahman M. Mizanur et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A Bifunctional Enzyme with L-Fucokinase and GDP-L-fucose Pyrophosphorylase Activities Salvages Free L-Fucose inArabidopsis

(2008) Toshihisa Kotake et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Metabolic engineering of Escherichia coli for biosynthesis of hyaluronic acid

(2007) Huimin Yu et al.   METABOLIC ENGINEERING