Article
Chemistry, Multidisciplinary
Gael M. Vos, Yunfei Wu, Roosmarijn van der Woude, Robert P. de Vries, Geert-Jan Boons
Summary: This paper introduces a chemo-enzymatic strategy for synthesizing poly-N-acetyl lactosamines (polyLacNAc) in a regioselective manner. The strategy involves enzymatic assembly of oligo-LacNAc chains and modification at specific positions using traceless blocking groups. The methodology allows for the synthesis of polyLacNAc chains with various topologies.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Ignacio alvarez-Martinez, Colin Ruprecht, Deborah Senf, Hsin-tzu Wang, Breeanna R. Urbanowicz, Fabian Pfrengle
Summary: Plant research is hindered by the lack of pure oligosaccharide samples that accurately represent cell wall glycans. To overcome this, glycosynthases can be used for chemo-enzymatic synthesis of oligosaccharides, allowing coupling reactions between oligosaccharide donors and acceptors. In this study, xylo-oligosaccharides up to dodecasaccharides were synthesized using glycosynthase-mediated coupling reactions. Important enzymes involved in xylan biosynthesis were identified and their activities were studied using biochemical assays.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biology
Yen-Pang Hsu, Deeptak Verma, Shuwen Sun, Caroline McGregor, Ian Mangion, Benjamin F. Mann
Summary: The study introduces a solid-phase glycan remodeling platform for enhancing the glycoengineering of immobilized antibodies. By evaluating over 30 enzymes, the research demonstrates the successful remodeling of IgG glycans into complex-type N-glycoforms with high yield and efficiency while preserving native binding affinity.
COMMUNICATIONS BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Amarnath Bollu, Aileen Peters, Andrea Rentmeister
Summary: The central dogma of molecular biology relies on messenger RNA (mRNA) for the conversion of genetic information. This article highlights the contribution of chemical-enzymatic modification of mRNA at the 5' cap and discusses various methods and applications for modifying mRNA.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Agricultural Engineering
Elena M. Rojo, Irene Piedra, Alicia M. Gonzalez, Marisol Vega, Silvia Bolado
Summary: The study compared the effects of different enzymes on the solubilization and recovery of components from microalgae-bacteria grown in piggery wastewater and microalgae grown in synthetic media by enzymatic hydrolysis. Results showed higher carbohydrate solubilizations from microalgae-bacteria compared to pure microalgae, and Proteases solubilized xylose remarkably well. Alcalase hydrolysis provided the highest peptide recovery, while Protamex resulted in low peptide recoveries but larger peptide sizes.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Vanessa Harawa, Thomas W. Thorpe, James R. Marshall, Jack J. Sangster, Amelia K. Gilio, Lucian Pirvu, Rachel S. Heath, Antonio Angelastro, James D. Finnigan, Simon J. Charnock, Jordan W. Nafie, Gideon Grogan, Roger C. Whitehead, Nicholas J. Turner
Summary: The development of efficient and sustainable methods for the synthesis of nitrogen heterocycles is a key goal for the chemical industry. This study presents a chemo-enzymatic approach for the synthesis of substituted piperidines, combining chemical synthesis and biocatalysis. This approach has proven useful for the synthesis of antipsychotic drugs and important intermediates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Review
Chemistry, Multidisciplinary
Benjamin Emenike, Ogonna Nwajiobi, Monika Raj
Summary: Nature increases the functional diversity of the proteome through posttranslational modifications (PTMs), which can be correlated to diseases. Therefore, it is important to qualitatively and quantitatively detect these transformations. One effective method is to use chemical strategies to label different PTMs, facilitating the study of their roles.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kazem Mahmoudzadeh, Zohreh Habibi, Maryam Yousefi, Mostafa Mostafavi, Mehdi Mohammadi
Summary: Increasing attention has been given to peptides as potential therapeutics, leading to the search for new peptide synthetic approaches and modifications. Enzymes have recently gained attention as a novel strategy and powerful tool for peptide and protein ligation. This study presents a fully convergent chemoenzymatic peptide synthesis process for the synthesis of aviptadil, a therapeutic peptide, using different peptiligase variants in aqueous environments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemical Research Methods
Felix Kaspar, Anett Schallmey
Summary: Enzymes are recognized as valuable tools in synthetic chemistry, enabling transformations that conventional organochemical approaches cannot achieve. Their expanding role in biocatalysis has introduced new opportunities for synthetic strategies and retrosynthetic disconnections. This review highlights recent literature examples to demonstrate the strategic uses of enzymes in natural product synthesis and advocates for their inclusion in the standard repertoire for complex small-molecule synthesis.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tobias M. Milzarek, Tobias A. M. Gulder
Summary: This article describes the first total synthesis of fungal sorbicillinoids spirosorbicillinols A-C, with a key step being a Diels-Alder cycloaddition reaction. The work provides a method for synthesizing all natural spirosorbicillinols and unnatural diastereomers.
COMMUNICATIONS CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Tsvetelina Batsalova, Balik Dzhambazov
Summary: Collagen type II (COL2) is significantly affected by autoimmune responses in rheumatoid arthritis (RA), and posttranslational modifications (PTMs) of COL2 have been implicated in RA autoimmunity. The discovery of anti-citrullinated protein response in RA has led to improved diagnostic assays and classification criteria for the disease. Immunological tolerance induced by modified COL2 peptides is considered as a potential strategy for RA therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yashapal Singh, Samira Escopy, Melanie Shadrick, Mithila D. Bandara, Keith J. Stine, Alexei V. Demchenko
Summary: Human milk oligosaccharides (HMO) are an active area of research in glycoscience and nutrition due to their involvement in infant development and potential disease prevention. This study reports the chemical synthesis of two core hexasaccharides found in human milk, pLNH and pLNnH, using a 3+3 convergent coupling strategy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Seonock Kim, Nitee Kumari, Jongwon Lim, Sateesh Dubbu, Amit Kumar, In Su Lee
Summary: A new nanodevice called SiJARs has been developed for enantioselective synthesis inside cells, showing high activity and enantioselectivity. This device is able to house metal nanocrystals and enzymes, creating an artificial catalytic organelle within living cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Dawid Hernik, Ewa Szczepanska, Maria Chiara Ghezzi, Elisabetta Brenna, Aleksandra Wloch, Hanna Pruchnik, Malwina Mularczyk, Krzysztof Marycz, Teresa Olejniczak, Filip Boratynski
Summary: This study aims to develop an efficient method for synthesizing oxygenated derivatives and evaluate their biological activities. A two-step chemo-enzymatic method was proposed, which involved lipase catalyzed epoxidation and microbial oxidation. The synthesized compounds showed potential antifungal, antioxidant, and antiproliferative activities.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuta Maki, Yuiko Otani, Ryo Okamoto, Masayuki Izumi, Yasuhiro Kajihara
Summary: High-mannose type glycans play significant roles in the biosynthesis and quality control of glycoproteins. In this study, we isolated and confirmed the identical structures of M5-glycan and M6-glycan to human-type glycans, suggesting their potential use in glycan analysis and the semisynthesis of glycoconjugates.
CARBOHYDRATE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Yuki Sasaki, Masahiro Komeno, Akihiro Ishiwata, Ayako Horigome, Toshitaka Odamaki, Jin-Zhong Xiao, Katsunori Tanaka, Yukishige Ito, Kanefumi Kitahara, Hisashi Ashida, Kiyotaka Fujita
Summary: Gum arabic arabinogalactan protein (AGP) can be effectively degraded by the cooperative action of GAfase, exo-beta 1,3-galactanase (Bl1,3Gal), and BlArafE, leading to the production of alpha-L-Rhap-(1 -> 4)-beta-D-GlcpA-(1-6)-beta-D-Galp-(1 -> 6)-D-Gal tetrasaccharide. This oligosaccharide can be utilized by commensal bacteria, potentially facilitating cross-feeding interactions between bifidobacteria and other gut microbial groups.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Midori Ikezaki, Kazuchika Nishitsuji, Ko Matsumura, Shino Manabe, Yukinao Shibukawa, Yoshinao Wada, Yukishige Ito, Yoshito Ihara
Summary: This study investigated the effects of C-mannosylation on the biological function of WSPW peptides and found that C-mannosylated WSPW peptides could interact with actinin-4, influencing the expression and subcellular localization of E-cadherin in cells.
Article
Biochemistry & Molecular Biology
Wataru Miyanishi, Makoto Ojika, Dai Akase, Misako Aida, Yasuhiro Igarashi, Yukishige Ito, Yu Nakagawa
Summary: The study found that attachment of 2-aminoethanol to the carboxy group of PRM-A significantly suppressed aggregation, and the prepared amide derivatives not only bound to Man without aggregation but also exhibited antifungal activity.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kazuchika Nishitsuji, Midori Ikezaki, Shino Manabe, Kenji Uchimura, Yukishige Ito, Yoshito Ihara
Summary: C-Mannosylation affects the biological functions of proteins, especially in thrombospondin 1 repeats. C-Mannosylated WSPW peptide inhibits TGF-beta-mediated synaptogenesis by suppressing Smad 2 activation. The molecular details of how C-mannosylation affects the biological functions of WxxW motifs require further study for clarification.
GLYCOCONJUGATE JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Takashi Kikuma, Haruka Ibuki, Masaya Nakamoto, Akira Seko, Yukishige Ito, Yoichi Takeda
Summary: It has been discovered that EDEM3 plays a significant role in the protein quality control in the endoplasmic reticulum (ER), by converting specific glycans into other types of glycans. Additionally, the activity of EDEM3 is enhanced through interaction with ERp46. This study provides new insights into the enzymatic properties of EDEM3 and the use of artificial glycan substrates to study ERAD mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Organic
Yu Nakagawa, Yuzuka Oya, Makoto Ojika, Yasuhiro Igarashi, Yukishige Ito
Summary: This study discovered that the modified derivatives of C4'-N-formyl PRM-A and C4'-N-acetyl PRM-A showed significant antifungal activities without forming aggregates or precipitates, providing a new strategy for the therapeutic application of PRM-A.
Article
Biochemistry & Molecular Biology
Sayaka Higashi, Yuki Imamura, Takashi Kikuma, Takahiro Matoba, Saya Orita, Yoshiki Yamaguchi, Yukishige Ito, Yoichi Takeda
Summary: The details of the complex formation between SelenoF and HUGT1 in the endoplasmic reticulum glycoprotein quality control system are still unclear. This study used co-transfection and crosslinking experiments to reveal the binding position of SelenoF to HUGT1, further elucidating the function of SelenoF.
Article
Chemistry, Multidisciplinary
Junpei Abe, Yoichi Takeda, Takashi Kikuma, Yasuhiko Kizuka, Hiroyuki Kajiura, Yasuhiro Kajihara, Yukishige Ito
Summary: UDP-Glc：glycoprotein glucosyltransferase (UGGT) plays a central role in maintaining the quality control of correctly folded N-glycoproteins in the endoplasmic reticulum (ER). However, a selective and potent inhibitor against UGGT has not been identified. In this study, we report squaryl group-modified UDP analogs as potential UGGT inhibitors, with one compound showing the highest potency and suggesting a candidate for further optimization.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Joichiro Nishio, Yoshiaki Takahashi, Masahiro Kasahara, Yoichi Takeda, Takashi Kikuma
Summary: A newly identified protein AeiA was found to be involved in selective autophagy and interacted with the autophagy-related protein Atg8 in Aspergillus oryzae. It was found to localize to autophagic membrane structures and peroxisomes. The disruption of AeiA resulted in reduced peroxisomal trafficking into the vacuole.
Article
Nutrition & Dietetics
Naoki Kodama, Hikaru Okada, Masakazu Hachisu, Miki Ando, Naoto Ito, Kazuki Nagata, Mayuka Katagiri, Yayoi Yasuda, Ikumi Hiroki, Takuya Yashiro, Gaku Ichihara, Masayuki Yamamoto, Chiharu Nishiyama
Summary: In this study, beta-damascone, a major ingredient of rose fragrance, was found to suppress DC-mediated immune responses by activating the NRF2 pathway in DCs, indicating its potential for the prevention and/or attenuation of immune-mediated diseases.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Organic
Yuki Kitaura, Rina Ueshima, Kanae Sano, Takashi Kikuma, Yoichi Takeda
Summary: This study demonstrated the efficient synthesis of CDP-DAG, a crucial precursor in forming lipid membranes, using sulfonylimidazolium salt as an activator. The method provides valuable insights into the biosynthesis and regulatory mechanisms of glycerophospholipids.
TETRAHEDRON LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Shun Maruyama, Kota Sawano, Satoko Amaki, Takehiro Suzuki, Satoru Narita, Kenta Kimura, Takatoshi Arakawa, Chihaya Yamada, Yukishige Ito, Naoshi Dohmae, Kiyotaka Fujita, Akihiro Ishiwata, Shinya Fushinobu
Summary: This study investigated the catalytic mechanism and substrate binding structure of the beta-l-arabinofuranosidase HypBA1 from Bifidobacterium longum. Zn2+ ion and cysteine were found to play key roles in catalysis, and the mode of substrate binding affected the catalytic activity.
