Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry

The impact of different human IgG capture molecules on the kinetics analysis of antibody-antigen interaction

(2020) Vishal Kamat et al.   ANALYTICAL BIOCHEMISTRY

The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context

(2020) Stefan Schüchner et al.   Science Signaling

Application of Lectin Microarrays for Biomarker Discovery

(2020) Kai Dang et al.   ChemistryOpen

Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium

(2020) Inga Baasch Christensen et al.   Fluids and Barriers of the CNS

Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates

(2020) Meng Xu et al.   PROTEIN EXPRESSION AND PURIFICATION

Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

(2019) Animesh Sarker et al.   Microbial Cell Factories

Utilizing the Synergistic Power of Molecular Theory and Molecular Simulation to Solve Bioengineering Problems

(2019) Mark Uline   BIOPHYSICAL JOURNAL

An efficient expression tag library based on self-assembling amphipathic peptides

(2019) Weixin Zhao et al.   Microbial Cell Factories

Pick a Tag and Explore the Functions of Your Pet Protein

(2019) Giel Vandemoortele et al.   TRENDS IN BIOTECHNOLOGY

Structure and functional analysis of a bacterial adhesin sugar-binding domain

(2019) Tyler D. R. Vance et al.   PLoS One

Proliferating cell nuclear antigen-agarose column: A tag-free and tag-dependent tool for protein purification affinity chromatography

(2019) Muhammad Tehseen et al.   JOURNAL OF CHROMATOGRAPHY A

Design and preparation of self-driven BSA surface imprinted tubular carbon nanofibers and their specific adsorption performance

(2019) Zuoting Yang et al.   CHEMICAL ENGINEERING JOURNAL

Automated Spatially Targeted Optical Microproteomics (autoSTOMP) to Determine Protein Complexity of Subcellular Structures

(2019) Bocheng Yin et al.   ANALYTICAL CHEMISTRY

Purification and immobilization of α-amylase in one step by gram-positive enhancer matrix (GEM) particles from the soluble protein and the inclusion body

(2019) Fangkun Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High yield recombinant expression and purification of oncogenic NSD1, NSD2, and NSD3 with human influenza hemagglutinin tag

(2019) Yunpeng Shen et al.   PROTEIN EXPRESSION AND PURIFICATION

Latest developments in the detection and separation of bovine serum albumin using molecularly imprinted polymers

(2019) Ali Jahanban-Esfahlan et al.   TALANTA

Passenger sequences can promote interlaced dimers in a common variant of the maltose-binding protein

(2019) Afaque A. Momin et al.   Scientific Reports

Plasmodium falciparum Blood Stage Antimalarial Vaccines: An Analysis of Ongoing Clinical Trials and New Perspectives Related to Synthetic Vaccines

(2019) David Ricardo Salamanca et al.   Frontiers in Microbiology

Bacterial sugar-binding protein as a one-step affinity purification tag on dextran-containing resins

(2019) Brett Kinrade et al.   PROTEIN EXPRESSION AND PURIFICATION

Aptamer affinity ligands in protein chromatography

(2018) Gérald Perret et al.   BIOCHIMIE

New trends in aggregating tags for therapeutic protein purification

(2018) Xiaofeng Yang et al.   BIOTECHNOLOGY LETTERS

A split-intein-based method for the efficient production of circularized nanodiscs for structural studies of membrane proteins

(2018) Miehling Jonas et al.   CHEMBIOCHEM

Functionalized gold nanoparticles as affinity nanoprobes for multiple lectins

(2018) Karuppuchamy Selvaprakash et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

A multifunctional tag with the ability to benefit the expression, purification, thermostability and activity of recombinant proteins

(2018) Weixin Zhao et al.   JOURNAL OF BIOTECHNOLOGY

Aptamer facilitated purification of functional proteins

(2018) Stanislav S. Beloborodov et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Extraction, purification and physicochemical properties of a novel lectin from Laetiporus sulphureus mushroom

(2018) Yufeng Wang et al.   LWT-FOOD SCIENCE AND TECHNOLOGY

Functionalized Nano-adsorbent for Affinity Separation of Proteins

(2018) Xueyan Zou et al.   Nanoscale Research Letters

A SEP tag enhances the expression, solubility and yield of recombinant TEV protease without altering its activity

(2018) Kalpana Nautiyal et al.   New Biotechnology

A new pH-responsive peptide tag for protein purification

(2018) Takahiro Nonaka et al.   PROTEIN EXPRESSION AND PURIFICATION

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags

(2018) Sarah C. Bernier et al.   PROTEIN EXPRESSION AND PURIFICATION

Lifetime and Aging of Chromatography Resins during Biopharmaceutical Manufacture

(2018) Mauryn C. Nweke et al.   TRENDS IN BIOTECHNOLOGY

Tag Array gene chip rapid diagnosis anti-tuberculosis drug resistance in pulmonary tuberculosis -a feasibility study

(2018) Wenjie Wu et al.   TUBERCULOSIS

Conditional Protein Splicing Switch in Hyperthermophiles through an Intein-Extein Partnership

(2018) Christopher W. Lennon et al.   mBio

Tailoring recombinant lipases: keeping the His-tag favors esterification reactions, removing it favors hydrolysis reactions

(2018) Janaina Marques de Almeida et al.   Scientific Reports

Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags

(2018) Yamin Fan et al.   Polymers

Carbon Nanotubes: Classification, Method of Preparation and Pharmaceutical Application

(2018) Anuja Rode et al.   Current Drug Delivery

Functionalized Nano-adsorbent for Affinity Separation of Proteins

(2018) Xueyan Zou et al.   Nanoscale Research Letters

Heparin: role in protein purification and substitution with animal-component free material

(2018) Svenja Nicolin Bolten et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Identification of an alternative translation initiation site in the sequence of the commonly used Glutathione S-Transferase tag

(2018) Sarah C. Bernier et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System

(2018) Won Gwak et al.   Viruses-Basel

A histidine-rich elastin-like polypeptide functions as a quickly detectable and easily purifiable protein fusion tag

(2018) Wen-Xin Geng et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells

(2018) Mitra S. Rana et al.   BIOCHEMISTRY

CRDSAT Generated by pCARGHO: A New Efficient Lectin-Based Affinity Tag Method for Safe, Simple, and Low-Cost Protein Purification

(2018) Alexandre Kriznik et al.   Biotechnology Journal

Uncovering new disease indications for G-protein coupled receptors and their endogenous ligands

(2018) Johannes M Freudenberg et al.   BMC BIOINFORMATICS

Analysis of KRAS, NRAS and BRAF mutational profile by combination of in-tube hybridization and universal tag-microarray in tumor tissue and plasma of colorectal cancer patients

(2018) Francesco Damin et al.   PLoS One

Delineating WWOX Protein Interactome by Tandem Affinity Purification-Mass Spectrometry: Identification of Top Interactors and Key Metabolic Pathways Involved

(2018) Tabish Hussain et al.   Frontiers in Oncology

Protein A- and Protein G-gold nanoparticle bioconjugates as nano-immunoaffinity platform for human IgG depletion in plasma and antibody extraction from cell culture supernatant

(2018) Siyao Liu et al.   TALANTA

Protein purification and analysis: next generation Western blotting techniques

(2017) Manish Mishra et al.   Expert Review of Proteomics

A visual screen for diet-regulated proteins in the Drosophila ovary using GFP protein trap lines

(2017) Hwei-Jan Hsu et al.   GENE EXPRESSION PATTERNS

Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal ‘C-tag’

(2017) Jing Jin et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Application of an E. coli signal sequence as a versatile inclusion body tag

(2017) Wouter S. P. Jong et al.   Microbial Cell Factories

CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag

(2017) Hiroyuki Takeda et al.   PLoS One

Affinity purification of Car9-tagged proteins on silica matrices: Optimization of a rapid and inexpensive protein purification technology

(2017) Jessica Soto-Rodríguez et al.   PROTEIN EXPRESSION AND PURIFICATION

Going native: Complete removal of protein purification affinity tags by simple modification of existing tags and proteases

(2017) Hui Chin Goh et al.   PROTEIN EXPRESSION AND PURIFICATION

Metal ions-binding T4 lysozyme as an intramolecular protein purification tag compatible with X-ray crystallography

(2017) Evzen Boura et al.   PROTEIN SCIENCE

Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

(2017) Kouroush Salimi et al.   RSC Advances

A cleavable self-assembling tag strategy for preparing proteins and peptides with an authentic N-terminus

(2017) Qing Zhao et al.   Biotechnology Journal

Immuno-affinity purification of 2B8-tagged proteins

(2017) Hyerin Choi et al.   Applied Biological Chemistry

G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity

(2017) Kasumi Tatsumi et al.   Scientific Reports

Sodium fluoride (NaF) induces the splenic apoptosis via endoplasmic reticulum (ER) stress pathway in vivo and in vitro

(2016) Huidan Deng et al.   Aging-US

Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368)

(2016) Dongxin Zhao et al.   BIOINORGANIC CHEMISTRY AND APPLICATIONS

Elastin-like polypeptides: A strategic fusion partner for biologics

(2016) Agnes Yeboah et al.   BIOTECHNOLOGY AND BIOENGINEERING

In vivo phosphorylation of a peptide tag for protein purification

(2016) Marine Goux et al.   BIOTECHNOLOGY LETTERS

Simultaneous detection of assembly and disassembly of multivalent HA tag and anti-HA antibody in single in-capillary assay

(2016) Jianhao Wang et al.   ELECTROPHORESIS

Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag

(2016) Mohamed A.A. Abdelhamid et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Evaluation of employing poly-lysine tags versus poly-histidine tags for purification and characterization of recombinant copper-binding proteins

(2016) Chathuri J.K. Wijekoon et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins

(2016) Marcel Bokhove et al.   JOURNAL OF STRUCTURAL BIOLOGY

Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF

(2016) J. Enrique Cantu-Bustos et al.   PROTEIN EXPRESSION AND PURIFICATION

Heparin-binding peptide as a novel affinity tag for purification of recombinant proteins

(2016) Jacqueline Morris et al.   PROTEIN EXPRESSION AND PURIFICATION

Single-step affinity and cost-effective purification of recombinant proteins using the Sepharose-binding lectin-tag from the mushroom Laetiporus sulphureus as fusion partner

(2016) Xiao-jing Li et al.   PROTEIN EXPRESSION AND PURIFICATION

Npro fusion technology: On-column complementation to improve efficiency in biopharmaceutical production

(2016) S. Schindler et al.   PROTEIN EXPRESSION AND PURIFICATION

Crystal structures of MBP fusion proteins

(2016) David S. Waugh   PROTEIN SCIENCE

High-throughput recombinant protein expression inEscherichia coli: current status and future perspectives

(2016) Baolei Jia et al.   Open Biology

Role of Recombinant DNA Technology to Improve Life

(2016) Suliman Khan et al.   International Journal of Genomics

Construction and use of aCupriavidus necatorH16 soluble hydrogenase promoter (PSH) fusion togfp(green fluorescent protein)

(2016) Bat-Erdene Jugder et al.   PeerJ

AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis

(2016) Tomoya Yano et al.   PLoS One

Production of full-length soluble Plasmodium falciparum RH5 protein vaccine using a Drosophila melanogaster Schneider 2 stable cell line system

(2016) Kathryn A. Hjerrild et al.   Scientific Reports

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

(2015) Nur Royhaila Mohamad et al.   BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

Extraction and downstream processing of plant-derived recombinant proteins

(2015) J.F. Buyel et al.   BIOTECHNOLOGY ADVANCES

Aggregating tags for column-free protein purification

(2015) Zhanglin Lin et al.   Biotechnology Journal

The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors

(2015) Rachael S. Felberbaum   Biotechnology Journal

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances

(2015) Marco Y. Hein et al.   CELL

Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1

(2015) Yangchao Dong et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Imidazole-free purification of His 3 -tagged recombinant proteins using ssDNA aptamer-based affinity chromatography

(2015) Filip Bartnicki et al.   JOURNAL OF CHROMATOGRAPHY A

Mild and cost-effective green fluorescent protein purification employing small synthetic ligands

(2015) Ana Sofia Pina et al.   JOURNAL OF CHROMATOGRAPHY A

Development of an aptamer-affinity chromatography for efficient single step purification of Concanavalin A from Canavalia ensiformis

(2015) Rajesh Ahirwar et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Protein cold adaptation: Role of physico-chemical parameters in adaptation of proteins to low temperatures

(2015) Soheila Shokrollahzade et al.   JOURNAL OF THEORETICAL BIOLOGY

Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli

(2015) Somaya Zahran et al.   PROTEIN EXPRESSION AND PURIFICATION

Aggregating tags for column-free protein purification

(2015) Zhanglin Lin et al.   Biotechnology Journal

The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors

(2015) Rachael S. Felberbaum   Biotechnology Journal

Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling

(2015) Dawson Fogen et al.   PLoS One

A cleavable silica-binding affinity tag for rapid and inexpensive protein purification

(2014) Brandon L. Coyle et al.   BIOTECHNOLOGY AND BIOENGINEERING

Challenges and recent advances in affinity purification of tag-free proteins

(2014) Dongli Guan et al.   BIOTECHNOLOGY LETTERS

Recombinant expression of antimicrobial peptides using a novel self-cleaving aggregation tag in Escherichia coli

(2014) Chao Luan et al.   CANADIAN JOURNAL OF MICROBIOLOGY

New trends and affinity tag designs for recombinant protein purification

(2014) David W Wood   CURRENT OPINION IN STRUCTURAL BIOLOGY

Intein Applications: From Protein Purification and Labeling to Metabolic Control Methods

(2014) David W. Wood et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids

(2014) Jia Fan et al.   Nanomedicine-Nanotechnology Biology and Medicine

Correction: New Molecular Reporters for Rapid Protein Folding Assays

(2014) Stéphanie Cabantous et al.   PLoS One

The cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C

(2014) Peter M. Hwang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PA tag: A versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin

(2014) Yuki Fujii et al.   PROTEIN EXPRESSION AND PURIFICATION

Immunoreaction of a recombinant nanobody from camelid single domain antibody fragment with Acinetobacter baumannii

(2014) Z. Payandeh et al.   TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

(2014) Sofia Costa et al.   Frontiers in Microbiology

Split intein mediated ultra-rapid purification of tagless protein (SIRP)

(2013) Dongli Guan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of prone-to-aggregate proteins

(2013) Mario Lebendiker et al.   FEBS LETTERS

Peptide Tag/Probe Pairs Based on the Coordination Chemistry for Protein Labeling

(2013) Shohei Uchinomiya et al.   INORGANIC CHEMISTRY

Nano-Liquid Chromatography in Pharmaceutical and Biomedical Research

(2013) M. R. Gama et al.   JOURNAL OF CHROMATOGRAPHIC SCIENCE

Carbon-Binding Designer Proteins that Discriminate between sp2- and sp3-Hybridized Carbon Surfaces

(2013) Brandon L. Coyle et al.   LANGMUIR

Heparin–protein interactions: From affinity and kinetics to biological roles. Application to an interaction network regulating angiogenesis

(2013) Franck Peysselon et al.   MATRIX BIOLOGY

Measurement of Cetuximab and Panitumumab-Unbound Serum EGFR Extracellular Domain Using an Assay Based on Slow Off-Rate Modified Aptamer (SOMAmer) Reagents

(2013) Noh Jin Park et al.   PLoS One

Structural basis for substrate specificity in the Escherichia coli maltose transport system

(2013) M. L. Oldham et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein

(2013) Takeshi Sangawa et al.   PROTEIN SCIENCE

To fuse or not to fuse: What is your purpose?

(2013) Mark R. Bell et al.   PROTEIN SCIENCE

OptimizedE. coliexpression strain LOBSTR eliminates common contaminants from His-tag purification

(2013) Kasper R. Andersen et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Several Affinity Tags Commonly Used in Chromatographic Purification

(2013) Xinyu Zhao et al.   Journal of Analytical Methods in Chemistry

Immobilization of unraveled immunoglobulin G using well-oriented ZZ–His protein on functionalized microtiter plate for sensitive immunoassay

(2012) Hong-Ming Yang et al.   ANALYTICAL BIOCHEMISTRY

Characterization of the diatomite binding domain in the ribosomal protein L2 from E. coli and functions as an affinity tag

(2012) Junhua Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Production, purification, and characterization of the cecropin from Plutella xylostella, pxCECA1, using an intein-induced self-cleavable system in Escherichia coli

(2012) Hong Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cholesterol transport via ABCA1: New insights from solid-phase binding assay

(2012) Emmanuelle Reboul et al.   BIOCHIMIE

A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli

(2012) Jeong-Mi Moon et al.   BIOTECHNOLOGY LETTERS

Reinventing Affinity Tags

(2012) Pim Hermans et al.   Genetic Engineering & Biotechnology News

Improved lectin ELISA for glycosylation analysis of biomarkers using PS-tag-fused single-chain Fv

(2012) Yoichi Kumada et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The “CPC Clip Motif”: A Conserved Structural Signature for Heparin-Binding Proteins

(2012) Marc Torrent et al.   PLoS One

The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated

(2012) Sreejith Raran-Kurussi et al.   PLoS One

Expression and purification of recombinant human tyrosine hydroxylase as a fusion protein in Escherichia coli

(2012) Colin A. Higgins et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression and purification of full length mouse metal response element binding transcription factor-1 using Pichia pastoris

(2012) Ryan W Huyck et al.   PROTEIN EXPRESSION AND PURIFICATION

Hydrophobically Assisted Separation-Friendly Mitsunobu Reaction

(2012) Xin-Shan Ye et al.   SYNLETT

Applications of aptamer affinity chromatography

(2012) Qiang Zhao et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

Antigenic and immunogenic properties of recombinant hemagglutinin proteins from H1N1 A/Brisbane/59/07 and B/Florida/04/06 when produced in various protein expression systems

(2012) Felix W. Santiago et al.   VACCINE

The role of polymer nanolayer architecture on the separation performance of anion-exchange membrane adsorbers: I. Protein separations

(2011) Bharat V. Bhut et al.   BIOTECHNOLOGY AND BIOENGINEERING

The tandem affinity purification technology: an overview

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Nonspecific Interaction between DNA and Protein allows for Cooperativity: A Case Study with Mycobacterium DNA Binding Protein

(2011) Abantika Ganguly et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli

(2011) Wei Wu et al.   Microbial Cell Factories

The structural basis of agonist-induced activation in constitutively active rhodopsin

(2011) Jörg Standfuss et al.   NATURE

Peptide and glycopeptide dendrimer apple trees as enzyme models and for biomedical applications

(2011) Jean-Louis Reymond et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

Imaging proteins inside cells with fluorescent tags

(2011) Georgeta Crivat et al.   TRENDS IN BIOTECHNOLOGY

Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag

(2011) Liping Huang et al.   VIRUS RESEARCH

Chromatography in Industry

(2010) Peter Schoenmakers   Annual Review of Analytical Chemistry

Commonly used tag combinations for tandem affinity purification

(2010) Yifeng Li   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Use of phage display methods to identify heptapeptide sequences for use as affinity purification ‘tags’ with novel chelating ligands in immobilized metal ion affinity chromatography

(2010) Jane T. Mooney et al.   JOURNAL OF CHROMATOGRAPHY A

Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

(2010) Thomas Heel et al.   Microbial Cell Factories

The Isoelectric Region of Proteins: A Systematic Analysis

(2010) Michael Widmann et al.   PLoS One

High-yield production and characterization of biologically active GST-tagged human topoisomerase IIα protein in insect cells for the development of a high-throughput assay

(2010) Praveen K. Singh et al.   PROTEIN EXPRESSION AND PURIFICATION

Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

(2010) J. R. A. Hutchins et al.   SCIENCE

Magnetic Nanocomposite Spheres Decorated with NiO Nanoparticles for a Magnetically Recyclable Protein Separation System

(2009) Jaeyun Kim et al.   ADVANCED MATERIALS

A Peptide Tag System for Facile Purification and Single-Molecule Immobilization

(2009) Jin Huang et al.   BIOCHEMISTRY

Analysis of the Human Cancer Glycome Identifies a Novel Group of Tumor-Associated N-Acetylglucosamine Glycan Antigens

(2009) T. Satomaa et al.   CANCER RESEARCH

A Carboxy-Terminal Affinity Tag for the Purification and Mass Spectrometric Characterization of Integral Membrane Proteins

(2009) Julie P. Wong et al.   JOURNAL OF PROTEOME RESEARCH

Development of an efficiently cleaved, bioactive, highly pure FLAG-tagged recombinant human Mullerian Inhibiting Substance

(2009) Thanos D. Papakostas et al.   PROTEIN EXPRESSION AND PURIFICATION

Controllable protein cleavages through intein fragment complementation

(2009) Gerrit Volkmann et al.   PROTEIN SCIENCE

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Fundamentals of Protein Separations: 50 Years of Nanotechnology, and Growing

(2008) David A. Egas et al.   Annual Review of Analytical Chemistry

Epitope tagging

(2008) Bill Brizzard   BIOTECHNIQUES

Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant Saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography

(2008) M. E. Lienqueo et al.   BIOTECHNOLOGY LETTERS

Application of micro- and nano-HPLC to the determination and characterization of bioactive and biomarker peptides

(2008) José María Saz et al.   JOURNAL OF SEPARATION SCIENCE

Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G

(2008) S. Miyakawa et al.   RNA