Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites

Structural Basis for Rab8a Recruitment of RILPL2 via LRRK2 Phosphorylation of Switch 2

(2020) Dieter Waschbüsch et al.   STRUCTURE

Advances in elucidating the function of leucine-rich repeat protein kinase-2 in normal cells and Parkinson's disease

(2020) Matthew Taylor et al.   CURRENT OPINION IN CELL BIOLOGY

“LRRK2: Autophagy and Lysosomal Activity”

(2020) Marta Madureira et al.   Frontiers in Neuroscience

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

(2020) Ying Fan et al.   Jove-Journal of Visualized Experiments

The In Situ Structure of Parkinson’s Disease-Linked LRRK2

(2020) Reika Watanabe et al.   CELL

LRRK 2 activation controls the repair of damaged endomembranes in macrophages

(2020) Susanne Herbst et al.   EMBO JOURNAL

Accurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson’s disease

(2020) Ozge Karayel et al.   MOLECULAR & CELLULAR PROTEOMICS

Structure of LRRK2 in Parkinson’s disease and model for microtubule interaction

(2020) C. K. Deniston et al.   NATURE

Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity

(2020) Alexia F. Kalogeropulou et al.   BIOCHEMICAL JOURNAL

MaxQuant.Live enables global targeting of more than 25,000 peptides

(2019) Christoph Wichmann et al.   MOLECULAR & CELLULAR PROTEOMICS

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44

(2019) Nazma Malik et al.   BIOCHEMICAL JOURNAL

The ProteomeXchange consortium in 2020: enabling ‘big data’ approaches in proteomics

(2019) Eric W Deutsch et al.   NUCLEIC ACIDS RESEARCH

PPM1H phosphatase counteracts LRRK2 signaling by selectively dephosphorylating Rab proteins

(2019) Kerryn Berndsen et al.   eLife

LRRK2 Biology from structure to dysfunction: research progresses, but the themes remain the same

(2019) Daniel C. Berwick et al.   Molecular Neurodegeneration

The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human

(2018) Rafeeq Mir et al.   BIOCHEMICAL JOURNAL

Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration

(2018) Ga Ram Jeong et al.   Molecular Neurodegeneration

LRRK2 kinase in Parkinson's disease

(2018) Dario R. Alessi et al.   SCIENCE

A novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics

(2018) Nicolai Bache et al.   MOLECULAR & CELLULAR PROTEOMICS

LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation

(2018) Eun-Jin Bae et al.   Nature Communications

A pathway for Parkinson’s Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain

(2018) Herschel S Dhekne et al.   eLife

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis

(2017) Martin Steger et al.   eLife

Phos-tag analysis of Rab10 phosphorylation by LRRK2: a powerful assay for assessing kinase function and inhibitors

(2016) G. Ito et al.   BIOCHEMICAL JOURNAL

The Perseus computational platform for comprehensive analysis of (prote)omics data

(2016) Stefka Tyanova et al.   NATURE METHODS

Innate immunity kinase TAK1 phosphorylates Rab1 on a hotspot for posttranslational modifications by host and pathogen

(2016) Rebecca S. Levin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphoproteomics reveals that Parkinson's disease kinase LRRK2 regulates a subset of Rab GTPases

(2016) Martin Steger et al.   eLife

A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards

(2014) Jacek R. Wiśniewski et al.   MOLECULAR & CELLULAR PROTEOMICS

Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach

(2014) Steven A. Carr et al.   MOLECULAR & CELLULAR PROTEOMICS

Suspension trapping (STrap) sample preparation method for bottom-up proteomics analysis

(2014) Alexandre Zougman et al.   PROTEOMICS

Parallel Reaction Monitoring for High Resolution and High Mass Accuracy Quantitative, Targeted Proteomics

(2012) Amelia C. Peterson et al.   MOLECULAR & CELLULAR PROTEOMICS

Ser1292 Autophosphorylation Is an Indicator of LRRK2 Kinase Activity and Contributes to the Cellular Effects of PD Mutations

(2012) Z. Sheng et al.   Science Translational Medicine

Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser910/Ser935, disruption of 14-3-3 binding and altered cytoplasmic localization

(2010) Nicolas Dzamko et al.   BIOCHEMICAL JOURNAL

14-3-3 binding to LRRK2 is disrupted by multiple Parkinson's disease-associated mutations and regulates cytoplasmic localization

(2010) R. Jeremy Nichols et al.   BIOCHEMICAL JOURNAL

Substrate specificity and inhibitors of LRRK2, a protein kinase mutated in Parkinson's disease

(2009) R. Jeremy Nichols et al.   BIOCHEMICAL JOURNAL

Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma

(2009) Terri A Addona et al.   NATURE BIOTECHNOLOGY