Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

Packing of apolar side chains enables accurate design of highly stable membrane proteins

(2019) Marco Mravic et al.   SCIENCE

An improved platform for functional assessment of large protein libraries in mammalian cells

(2019) Kenneth A Matreyek et al.   NUCLEIC ACIDS RESEARCH

Warfarin and vitamin K epoxide reductase: a molecular accounting for observed inhibition

(2018) Sangwook Wu et al.   BLOOD

Stabilization of warfarin-binding pocket of VKORC1 and VKORL1 by a peripheral region determines their different sensitivity to warfarin inhibition

(2018) G. Shen et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Multiplex assessment of protein variant abundance by massively parallel sequencing

(2018) Kenneth A. Matreyek et al.   NATURE GENETICS

Substrate-modulated unwinding of transmembrane helices in the NSS transporter LeuT

(2018) Patrick S. Merkle et al.   Science Advances

The EVcouplings Python framework for coevolutionary sequence analysis

(2018) Thomas A Hopf et al.   BIOINFORMATICS

Analysis of Large-Scale Mutagenesis Data To Assess the Impact of Single Amino Acid Substitutions

(2017) Vanessa E. Gray et al.   GENETICS

Transmembrane helix hydrophobicity is an energetic barrier during the retrotranslocation of integral membrane ERAD substrates

(2017) Christopher J. Guerriero et al.   MOLECULAR BIOLOGY OF THE CELL

A platform for functional assessment of large variant libraries in mammalian cells

(2017) Kenneth A. Matreyek et al.   NUCLEIC ACIDS RESEARCH

Analysis of Large-Scale Mutagenesis Data To Assess the Impact of Single Amino Acid Substitutions

(2017) Vanessa E. Gray et al.   GENETICS

Structured States of Disordered Proteins from Genomic Sequences

(2016) Agnes Toth-Petroczy et al.   CELL

The power of multiplexed functional analysis of genetic variants

(2016) Molly Gasperini et al.   Nature Protocols

Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer

(2016) Guomin Shen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Warfarin and vitamin K compete for binding to Phe55 in human VKOR

(2016) Katrin J Czogalla et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Interplay between hydrophobicity and the positive-inside rule in determining membrane-protein topology

(2016) Assaf Elazar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Warfarin traps human vitamin K epoxide reductase in an intermediate state during electron transfer

(2016) Guomin Shen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Warfarin and vitamin K compete for binding to Phe55 in human VKOR

(2016) Katrin J Czogalla et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Mutational scanning reveals the determinants of protein insertion and association energetics in the plasma membrane

(2016) Assaf Elazar et al.   eLife

The I-TASSER Suite: protein structure and function prediction

(2015) Jianyi Yang et al.   NATURE METHODS

AlteredEscherichia colimembrane protein assembly machinery allows proper membrane assembly of eukaryotic protein vitamin K epoxide reductase

(2015) Feras Hatahet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Arg98Trp mutation in human VKORC1 causing VKCFD2 disrupts a di-arginine-based ER retention motif

(2014) K. J. Czogalla et al.   BLOOD

AlignMe—a membrane protein sequence alignment web server

(2014) Marcus Stamm et al.   NUCLEIC ACIDS RESEARCH

A series of PDB-related databanks for everyday needs

(2014) Wouter G. Touw et al.   NUCLEIC ACIDS RESEARCH

Structures of an intramembrane vitamin K epoxide reductase homolog reveal control mechanisms for electron transfer

(2014) Shixuan Liu et al.   Nature Communications

A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library

(2013) Pankaj C. Jain et al.   ANALYTICAL BIOCHEMISTRY

A cellular system for quantitation of vitamin K cycle activity: structure-activity effects on vitamin K antagonism by warfarin metabolites

(2013) J. A. Haque et al.   BLOOD

Rules for the recognition of dilysine retrieval motifs by coatomer

(2013) Wenfu Ma et al.   EMBO JOURNAL

Evaluation of warfarin resistance using transcription activator-like effector nucleases-mediated vitamin K epoxide reductase knockout HEK293 cells

(2013) J.-K. Tie et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

ClinVar: public archive of relationships among sequence variation and human phenotype

(2013) Melissa J. Landrum et al.   NUCLEIC ACIDS RESEARCH

Three-Dimensional Structures of Membrane Proteins from Genomic Sequencing

(2012) Thomas A. Hopf et al.   CELL

Human Vitamin K Epoxide Reductase and Its Bacterial Homologue Have Different Membrane Topologies and Reaction Mechanisms

(2012) Jian-Ke Tie et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Effect of the VKORC1 D36Y variant on warfarin dose requirement and pharmacogenetic dose prediction

(2012) Husam Qasim et al.   THROMBOSIS AND HAEMOSTASIS

Protein targeting and degradation are coupled for elimination of mislocalized proteins

(2011) Tara Hessa et al.   NATURE

Protein 3D Structure Computed from Evolutionary Sequence Variation

(2011) Debora S. Marks et al.   PLoS One

Clinical and Genetic Determinants of Warfarin Pharmacokinetics and Pharmacodynamics during Treatment Initiation

(2011) Inna Y. Gong et al.   PLoS One

A Comprehensive Comparison of Transmembrane Domains Reveals Organelle-Specific Properties

(2010) Hayley J. Sharpe et al.   CELL

Novel Insight into the Mechanism of the Vitamin K Oxidoreductase (VKOR)

(2010) Mark A. Rishavy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of a bacterial homologue of vitamin K epoxide reductase

(2010) Weikai Li et al.   NATURE

VKORC1 Pharmacogenomics Summary

(2010) Ryan P. Owen et al.   Pharmacogenetics and Genomics

Vitamin K epoxide reductase prefers ER membrane-anchored thioredoxin-like redox partners

(2010) S. Schulman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An algorithm for managing warfarin resistance

(2009) O. OSINBOWALE et al.   CLEVELAND CLINIC JOURNAL OF MEDICINE

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS