Editorial Material
Biology
Isabel Stoetzel, Eva Kiermaier
Summary: The centrosome plays a crucial role in determining which branches extending from the body of microglia will successfully engulf and clear away dead neurons.
Article
Developmental Biology
Giuliano Ferrero, Magali Miserocchi, Elodie Di Ruggiero, Valerie Wittamer
Summary: The study reveals distinct roles of Csf1ra and Csf1rb in hematopoiesis, with Csf1rb identified as a crucial regulator of adult microglia development. It demonstrates that Csf1rb positively regulates HSC-derived myelopoiesis, leading to macrophage deficiency, including microglia, in mutant animals. Overall, this research contributes new insights into evolutionary aspects of Csf1 signaling and offers a unique framework for dissecting embryonic versus adult microglia in vivo.
Article
Immunology
Eman Soliman, John Leonard, Erwin Kristobal Gudenschwager Basso, Ilana Gershenson, Jing Ju, Jatia Mills, Caroline de Jager, Alexandra M. Kaloss, Mohamed Elhassanny, Daniela Pereira, Michael Chen, Xia Wang, Michelle H. Theus
Summary: The EphA4 receptor tyrosine kinase suppresses MERTK in the brain, restricting efferocytosis of microglia and monocytes/macrophages. This inhibition of the ERK/Stat6 pathway prevents the clearance of apoptotic debris in brain injury.
JOURNAL OF NEUROINFLAMMATION
(2023)
Review
Cell Biology
Gaetan Juban, Benedicte Chazaud
Summary: Efferocytosis, the engulfment of dead cells by macrophages, plays a crucial role in tissue repair post-injury by delineating the transition from the pro-inflammatory phase to the recovery phase. In the context of skeletal muscle regeneration, efferocytosis is essential for sterile tissue injury followed by complete regeneration, with molecular mechanisms and metabolic control playing a significant role in this process.
Article
Multidisciplinary Sciences
Wan-Ru Zhuang, Yunfeng Wang, Weidong Nie, Yao Lei, Chao Liang, Jiaqi He, Liping Zuo, Li-Li Huang, Hai-Yan Xie
Summary: A nanosystem has been developed to enhance antitumor immunity by inhibiting MerTK-mediated efferocytosis and capturing tumor-associated agents.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Linxiu Wu, Rongtao Xue, Jiahao Chen, Jin Xu
Summary: In this study, we generated zebrafish dock8 mutants using CRISPR/Cas9 and found that dock8 mutations attenuate microglia colonization in the zebrafish midbrain. Time-lapse imaging revealed reduced motility of macrophages in the dock8 mutants. We also discovered that cdc42/cdc42l genes, which encode the small GTPase activated by Dock8, regulate microglia colonization in zebrafish.
CELL DEATH DISCOVERY
(2022)
Article
Microbiology
Jacquelyn A. Nielson, J. Muse Davis
Summary: Cryptococcal meningitis, a fungal infection of the brain, is a major cause of death in people with uncontrolled HIV. This study used zebrafish larvae as a model host and found that microglia play multiple roles in the infection, including reinforcing the blood-brain barrier, clearing cryptococcal cells, and potentially participating in crossing via an unknown mechanism.
MICROBIOLOGY SPECTRUM
(2023)
Review
Medicine, Research & Experimental
Amir Tajbakhsh, Seyed Mohammad Gheibi hayat, Ahmad Movahedpour, Amir Savardashtaki, Reid Loveless, George E. Barreto, Yong Teng, Amirhossein Sahebkar
Summary: Efferocytosis plays a role in clearing apoptotic tumor cells but can be controversial in treatment-induced scenarios. Tumor cells can exploit efferocytosis to escape the immune response.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Guoqing Wang, Kunhong Zhong, Zeng Wang, Zongliang Zhang, Xin Tang, Aiping Tong, Liangxue Zhou
Summary: This review discusses the characteristics and functions of TAMs in GBM, and evaluates the current state of TAMs-targeting strategies. TAMs are logical therapeutic targets for GBM, and understanding how TAMs promote GBM progression is of great significance for new immune therapeutic approaches for GBM patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Hongbin Yang, Zhenyan Yu, Shuaishuai Ji, Jie Yan, Yan Kong, Qiang Huo, Zhijun Zhang, Yimin Niu, Yang Liu
Summary: This study reports the use of apoptotic chondrocyte membrane-coated metal-organic framework nanoparticles loaded with quercetin as immunomodulators for treating osteoarthritis. These nanoparticles, disguised as apoptotic bodies in the joints, can be easily phagocytized by synovial macrophages similarly to efferocytosis, and the released quercetin promotes the polarization of synovial macrophages to the M2 phenotype and inhibits the apoptosis of articular chondrocytes. In vivo results show that this Trojan horse strategy can extend the retention time of nanoparticles in osteoarthritis joints and improve treatment efficacy. This work provides a potential and universal platform for engineered cell membrane-coated nanoparticles to resolve inflammation by simulating specific inflammatory processes.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Cardiac & Cardiovascular Systems
Ellen C. Keeley, Han J. Li, Christopher R. Cogle, Eileen M. Handberg, C. Noel Bairey Merz, Carl J. Pepine
Summary: This study found that in female patients with CMD, plasma levels of resolvin D1 and maresin 1 were significantly lower, while levels of docosahexaenoic acid and 18-hydroxyeicosapentaenoic acid were significantly higher. This suggests that insufficient or ineffective production of SPM may play a role in the pathophysiology of CMD.
AMERICAN JOURNAL OF CARDIOLOGY
(2022)
Review
Immunology
Maaike Schilperoort, David Ngai, Santosh R. Sukka, Kleopatra Avrampou, Hongxue Shi, Ira Tabas
Summary: The process of macrophages engulfing dying cells, known as efferocytosis, is tightly regulated and involves sensing, binding, engulfment, and digestion of apoptotic cells. Efferocytosis not only prevents tissue necrosis and inflammation caused by secondary necrosis of dying cells, but also promotes pro-resolving signaling in macrophages, which is essential for tissue resolution and repair following injury or inflammation. The cargo released from apoptotic cells after their engulfment and digestion by macrophages, containing amino acids, nucleotides, fatty acids, and cholesterol, plays a crucial role in this pro-resolving reprogramming.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Ana Carolina Guerta Salina, Leticia de Aquino Penteado, Naiara Naiana Dejani, Ludmilla Silva-Pereira, Breno Vilas Boas Raimundo, Gabriel Ferranti Correa, Karen Cristina Oliveira, Leandra Naira Zambelli Ramalho, Medeton Mahoussi Michael Boko, Vania L. D. Bonato, C. Henrique Serezani, Alexandra Ivo Medeiros
Summary: The removal of dead cells through efferocytosis is crucial for resolving infections and preserving tissues. The efferocytosis of non-infected apoptotic cells promotes the production of anti-inflammatory macrophages, while the impact of efferocytosis of infected apoptotic cells on macrophage activation remains unknown.
Article
Medicine, Research & Experimental
Gufang Zhang, Qi Li, Weijie Tao, Pingping Qin, Jiali Chen, Huicui Yang, Jiaojiao Chen, Hua Liu, Qijun Dai, Xuechu Zhen
Summary: This study investigated the role of Sigma-1 receptor (Sig-1R) in the phagocytic activity of macrophages/microglia and its significance in neuroprotection and neurological function in stroke. Results showed that depletion of Sig-1R impaired the phagocytic activity and worsened brain damage and neurological defects in stroke. Transfer of intact Sig-1R bone marrow-derived macrophages restored the clearance activity of dead/dying neurons and improved functional recovery after cerebral ischemia. This study provides evidence of the pivotal role of Sig-1R in macrophage/microglia-mediated efferocytosis and highlights its neuroprotective mechanism in stroke.
Article
Genetics & Heredity
Alain Lescoat, Riccardo Leinardi, Kevin Pouxvielh, Yousof Yakoub, Marie Lelong, Amandine Pochet, Erwan Dumontet, Nessrine Bellamri, Erwan Le Tallec, Cristina Pavan, Francesco Turci, Christophe Paris, Francois Huaux, Valerie Lecureur
Summary: The study compared the effects of exposure to pristine amosite (pAmo) or milled amosite (mAmo) particles on lung damage, autoimmunity, and macrophage phenotype. The results showed that long-term exposure to pAmo may induce significant lung damage and autoimmune effects, probably through an alteration of macrophage phenotype, supporting the higher toxicity of entire amosite (pAmo) compared to grinded amosite. However, the effects of mAmo on macrophage function should not be neglected.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Multidisciplinary Sciences
Fengzhu Xiong, Nikolaus D. Obholzer, Ramil R. Noche, Sean G. Megason
Article
Cell Biology
Doris Lou Demy, Muriel Tauzin, Mylene Lancino, Veronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, Philippe Herbomel
JOURNAL OF CELL SCIENCE
(2017)
Article
Genetics & Heredity
Ranad Shaheen, Eissa Faqeih, Hanan E. Shamseldin, Ramil R. Noche, Asma Sunker, Muneera J. Alshammari, Tarfa Al-Sheddi, Nouran Adly, Mohammed S. Al-Dosari, Sean G. Megason, Muneera Al-Husain, Futwan Al-Mohanna, Fowzan S. Alkuraya
AMERICAN JOURNAL OF HUMAN GENETICS
(2012)
Article
Biochemical Research Methods
Doris Lou Demy, Zachary Ranta, Jean-Michel Giorgi, Magali Gonzalez, Philippe Herbomel, Karima Kissa
Article
Multidisciplinary Sciences
Christina M. Agapakis, Henrike Niederholtmeyer, Ramil R. Noche, Tami D. Lieberman, Sean G. Megason, Jeffrey C. Way, Pamela A. Silver
Article
Biochemical Research Methods
Kishore R. Mosaliganti, Ramil R. Noche, Fengzhu Xiong, Ian A. Swinburne, Sean G. Megason
PLOS COMPUTATIONAL BIOLOGY
(2012)
Article
Biology
Jonathan R. Volpatti, Yukari Endo, Jessica Knox, Linda Groom, Stephanie Brennan, Ramil Noche, William J. Zuercher, Peter Roy, Robert T. Dirksen, James J. Dowling
Article
Cell Biology
Doris Lou Demy, Maria Letizia Campanari, Raphael Munoz-Ruiz, Heather D. Durham, Benoit J. Gentil, Edor Kabashi
Article
Multidisciplinary Sciences
Lindsay Smith, Lacramioara Fabian, Almundher Al-Maawali, Ramil R. Noche, James J. Dowling
Article
Biochemistry & Molecular Biology
Sarah J. Smith, Lacramioara Fabian, Adeel Sheikh, Ramil Noche, Xiucheng Cui, Steven A. Moore, James J. Dowling
Summary: Congenital muscular dystrophy type 1A (MDC1A) is a common genetic disorder caused by mutations in the LAMA2 gene. In this study, researchers used a zebrafish model to investigate the role of lysosome distribution in MDC1A. They found that abnormal lysosome distribution was associated with fiber detachment and could be improved by overexpression of a transcription factor called transcription factor EB. This suggests that targeting lysosome function may be a potential therapeutic strategy for treating MDC1A.
HUMAN MOLECULAR GENETICS
(2022)
Article
Genetics & Heredity
Robert Lesurf, Abdelrahman Said, Oyediran Akinrinade, Jeroen Breckpot, Kathleen Delfosse, Ting Liu, Roderick Yao, Gabrielle Persad, Fintan McKenna, Ramil R. Noche, Winona Oliveros, Kaia Mattioli, Shreya Shah, Anastasia Miron, Qian Yang, Guoliang Meng, Michelle Chan Seng Yue, Wilson W. L. Sung, Bhooma Thiruvahindrapuram, Jane Lougheed, Erwin Oechslin, Tapas Mondal, Lynn Bergin, John Smythe, Shashank Jayappa, Vinay J. Rao, Jayaprakash Shenthar, Perundurai S. Dhandapany, Christopher Semsarian, Robert G. Weintraub, Richard D. Bagnall, Jodie Ingles, Marta Mele, Philipp G. Maass, James Ellis, Stephen W. Scherer, Seema Mital
Summary: This study analyzed WGS data of 209 pediatric CMP patients and 1953 replication genomes and exomes, and found that variants in new genes and regulatory elements of known CMP genes contribute significantly to early onset CMP.
NPJ GENOMIC MEDICINE
(2022)
Article
Developmental Biology
Doris Lou Demy, Anne-Lou Touret, Mylene Lancino, Muriel Tauzin, Lavinia Capuana, Constance Pierre, Philippe Herbomel
Summary: Trim33 is a critical regulator in hematopoiesis, playing important roles in the production of red blood cells and macrophages. Loss of Trim33 leads to premature aging of macrophages and excessive production of neutrophils and thrombocytes.
Article
Biochemistry & Molecular Biology
Rebecca Simonian, Emanuela Pannia, Rola Hammoud, Ramil R. Noche, Xiucheng Cui, Eva Kranenburg, Ruslan Kubant, Paula Ashcraft, Brandi Wasek, Teodoro Bottiglieri, James J. Dowling, G. Harvey Anderson
Summary: This study evaluated the effects of mthfr deficiency and folic acid supplementation on energy homeostasis and metabolism using a novel zebrafish model. The genetic loss of mthfr function in zebrafish resulted in lipid accumulation, aberrant cholesterol metabolism, and impaired energy homeostasis. Folic acid supplementation mimicked some of these effects and exacerbated them in mthfr-deficient zebrafish.
HUMAN MOLECULAR GENETICS
(2023)