Article
Multidisciplinary Sciences
Suman Gurung, Nicole K. Restrepo, Brendan Chestnut, Laurita Klimkaite, Saulius Sumanas
Summary: Vascular endothelial cells exhibit phenotypic and transcriptional heterogeneity during early embryogenesis, but the molecular mechanisms involved are not well understood. Using single-cell RNA sequencing, this study identified subtypes of vascular endothelial cells in zebrafish embryos at the 24 hpf stage, including arterial, venous, cranial, endocardial and endothelial progenitor cell subtypes. The findings provide valuable insights into the heterogeneity and cell-fate decisions of vascular endothelial cells.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Zulvikar Syambani Ulhaq, Delbert Almerick T. Boncan, Ting Fung Chan, William Ka Fai Tse
Summary: This study investigated the toxicity of PFHxS using a zebrafish model. One experiment demonstrated that PFHxS might affect glucose and fatty acid metabolism, leading to oxidative stress, developmental defects, and cell cycle arrest. Another experiment showed that exposure to PFHxS changed the concentrations of various metabolites.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Cell Biology
Anne Aalto, Adan Olguin-Olguin, Erez Raz
Summary: This article discusses the migration mechanism of zebrafish primordial germ cells during embryonic development, which involves a bleb-driven amoeboid migration mode controlled by chemical and physical cues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Lucas W. H. Sun, Halida Thanveer Asana Marican, Hongyuan Shen
Summary: Apoptosis, a regulated form of cell death, induced by ionizing radiation, can be effectively studied in intact organisms such as zebrafish. In this study, a transgenic zebrafish expressing a genetically encoded secreted protein was used to visualize and quantify radiation-induced apoptosis. The results demonstrate the usefulness of this system for investigating the dynamic process of radiation-induced apoptosis.
RADIATION RESEARCH
(2023)
Article
Environmental Sciences
Ziyi Wei, Weiguo Wang, Wenping Xu, Liming Tao, Zhong Li, Yang Zhang, Xusheng Shao
Summary: This study investigated the immunotoxic effects of Emamectin benzoate (EMB) on zebrafish embryos. The results showed that EMB exposure led to embryo mortality, delayed hatching, increased malformations, and decreased neutrophils and macrophages. Metabolomics analysis revealed abnormal changes in glycerophospholipid metabolism as the main mechanism of EMB-induced immunotoxicity.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Environmental Sciences
Suwen Zeng, Yuyang Peng, Jinze Ma, Yurui Ge, Yong Huang, Shuling Xie, Wei Yuan, Chen Lu, Hua Zhang, Qiang Luo, Xinjun Liao, Huiqiang Lu
Summary: Metalaxyl-M exerts developmental toxicity and immunotoxicity to zebrafish embryos, potentially through the regulation of the WNT signaling pathway.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Biochemistry & Molecular Biology
Benedikt Bauer, Angela Mally, Daniel Liedtke
Summary: Before conducting experiments involving living animals in biological laboratories, it is important to consider the necessity, ethics, and potential harm. The development of the 3R principle has become a key standard in animal experimentation worldwide, leading to the exploration of alternative methods in species like zebrafish to reduce experiments on mammals. Zebrafish, with their favorable biological properties, have shown promise in toxicological testing, particularly in cardio-, neuro, hepato- and nephrotoxicity studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Nathalie Geyer, Sabrina Kaminsky, Shir Confino, Zohar Ben-Moshe Livne, Yoav Gothilf, Nicholas S. Foulkes, Daniela Vallone
Summary: With the increasing use of fish as model species, cell cultures derived from caudal fin explants and pre-hatching embryos have become valuable in vitro tools for research and can serve as an ethical alternative to live animal experiments. However, existing protocols require homogeneous pools of embryos or viable adult fish, limiting the use of fish lines with adverse phenotypes or high early-stage mortality. This study presents a simple protocol to generate large-scale cell lines from individual early embryos, enabling genotyping by polymerase chain reaction and promoting the routine use of fish cell culture models for functional genetic studies.
LABORATORY ANIMALS
(2023)
Article
Environmental Sciences
Mengxi Cao, Jinbo Wei, Yu Pan, Ling Wang, Zhunjie Li, Yeli Hu, Yong Liang, Huiming Cao
Summary: Bisphenol A (BPA) and its analogues can disrupt the endocrine system and reproductive function. Recent studies have shown that some BPA analogues have strong anti-estrogenic effects, but conflicting results were observed in different in vitro assays, and it is still unclear whether these BPA analogues have in vivo effects at environmentally relevant concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Heidar Heidari Khoei, Alok Javali, Harunobu Kagawa, Theresa Maria Sommer, Giovanni Sestini, Laurent David, Jana Slovakova, Maria Novatchkova, Yvonne Scholte Op Reimer, Nicolas Rivron
Summary: Human blastoids, developed from stem cells, can accurately mimic the morphology and development of blastocysts. These blastoids can also replicate the transcriptional features of blastocysts and simulate the process of implantation into endometrial organoids under hormonal stimulation. It takes 4 days to form blastoids and an additional 10 days to prepare the endometrial implantation assay. With proper experience in stem cell culture and organoid culture, the protocol can be successfully performed. Blastoids offer an ethical alternative and an opportunity for scientific and biomedical discoveries in early pregnancy.
Article
Engineering, Environmental
Yuxuan Liu, Yonghua Wang, Xin Ling, Zhenhua Yan, Donghai Wu, Jianchao Liu, Guanghua Lu
Summary: Nanoplastics and BMDBM have significant effects on the gene expression in zebrafish embryos, with combined exposure reducing adverse effects on gene expression. Single-cell RNA sequencing technology identified neural mid cells as target cells of both pollutants, which commonly altered brain and head development functions.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Environmental Sciences
Marta Silva Muniz, Katharina Halbach, Igor Caue Alves Araruna, Rafael Xavier Martins, Bettina Seiwert, Oliver Lechtenfeld, Thorsten Reemtsma, Davi Farias
Summary: Moxidectin exhibited acute toxicity to zebrafish embryos by decreasing hatching rates, altering enzymatic activity, and strongly accumulating in embryos, mainly distributed in the head and eyes.
ENVIRONMENTAL POLLUTION
(2021)
Article
Oncology
Ziqi Zhou, Jing Zhao, Ke Hu, Xiaorong Hou, Xiansong Sun, Xiaoli Pan, Xiaohui Wang, Nan Li, Zhiwei Yang, Fuquan Zhang, Qianqian Zhou, Linsheng Zhan
Summary: This study systematically examined the dose effect of radiation therapy on the in vivo homing and distribution of bone marrow-derived dendritic cells using noninvasive imaging. The results showed that radiation did not directly cause cellular apoptosis or necrosis in DCs, but induced mitochondrial damage and upregulation of certain receptors. Higher radiation doses (>= 5 Gy) significantly increased the homing ability of DCs to lymphoid tissues, and this improvement was associated with reactive oxygen species accumulation. Enhanced T cell activation was also observed in mice inoculated with higher radiation dose-treated DCs, indicating the advantages of >= 5 Gy radiation in facilitating DC homing and T cell priming.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Cell Biology
Catarina Bota, Gabriel G. Martins, Susana S. Lopes
Summary: During vertebrate development, left-right symmetry breaking occurs in the left-right organizer (LRO). This study reveals a new link between somite specification and Dand5 function, demonstrating how left-right signals affect the behavior of zebrafish somite cell precursors. The findings show that Dand5 is involved in cell exit from the maturation zone into the presomitic mesoderm, affecting the expression patterns of myogenic factors and tail size.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Environmental Sciences
Qiang Luo, Shuqiong Tang, Xiaoping Xiao, You Wei, Bo Cheng, Yong Huang, Keyuan Zhong, Guiyou Tian, Huiqiang Lu
Summary: In this study, the toxicity of benomyl to zebrafish was evaluated, showing high toxicity and inducing oxidative stress and apoptosis. These findings provide important insights into the safe use of benomyl.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Cell Biology
Doris Lou Demy, Muriel Tauzin, Mylene Lancino, Veronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, Philippe Herbomel
JOURNAL OF CELL SCIENCE
(2017)
Correction
Cell Biology
Mai Nguyen-Chi, Beryl Laplace-Builhe, Jana Travnickova, Patricia Luz-Crawford, Gautier Tejedor, Georges Lutfalla, Karima Kissa, Christian Jorgensen, Farida Djouad
CELL DEATH & DISEASE
(2018)
Article
Biochemistry & Molecular Biology
Nausicaa Poullet, Ivan Golushko, Vladimir Lorman, Jana Travnickova, Charlotte Bureau, Dmitrii Chalin, Sergei Rochal, Andrea Parmeggiani, Karima Kissa
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Multidisciplinary Sciences
Gautier Tejedor, Beryl Laplace-Builhe, Patricia Luz-Crawford, Said Assou, Audrey Barthelaix, Marc Mathieu, Karima Kissa, Christian Jorgensen, Jerome Collignon, Paul Chuchana, Farida Djouad
SCIENTIFIC REPORTS
(2020)
Article
Pharmacology & Pharmacy
L. Simon, V. Lapinte, L. Lionnard, N. Marcotte, M. Morille, A. Aouacheria, K. Kissa, J. M. Devoisselle, S. Begu
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2020)
Article
Cell Biology
Doris Lou Demy, Maria Letizia Campanari, Raphael Munoz-Ruiz, Heather D. Durham, Benoit J. Gentil, Edor Kabashi
Article
Cell Biology
Doris Lou Demy, Mireille Carrere, Ramil Noche, Muriel Tauzin, Marion Le Bris, Chooyoung Baek, Ignaty Leshchiner, Wolfram Goessling, Philippe Herbomel
JOURNAL OF CELL SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Sasja Blokzijl-Franke, Bas Ponsioen, Stefan Schulte-Merker, Philippe Herbomel, Karima Kissa, Suma Choorapoikayil, Jeroen den Hertog
Summary: The study found that HSPCs in mutant zebrafish embryos lacking pten function exhibited death during development, which could be rescued by inhibiting PI3K, while inhibiting PI3K in wild-type embryos also induced HSPC death. PI3K signaling tightly controls HSPC survival, with both up- and downregulation of the signaling pathway reducing stemness of HSPCs.
Article
Multidisciplinary Sciences
Dmitrii Chalin, Charlotte Bureau, Andrea Parmeggiani, Sergei Rochal, Karima Kissa, Ivan Golushko
Summary: This study investigates the mechanism of blood cell formation and the role of mechanical stress in this process, highlighting the suitability of the zebrafish model for such studies. By developing a new DA micromechanical model, the researchers explored the mechanisms driving the shape changes of the DA and individual ECs.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jana Travnickova, Sandra Nhim, Naoill Abdellaoui, Farida Djouad, Mai Nguyen-Chi, Andrea Parmeggiani, Karima Kissa
Summary: This study uses zebrafish embryos to observe the migration behavior of macrophages in a physiological environment, showing that macrophages adopt different cell shapes and migrate through ECM. Inhibition of MMPs or Rac GTPase suppresses mesenchymal migration of macrophages and HSPC mobilization.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Lucile Plumet, Nour Ahmad-Mansour, Catherine Dunyach-Remy, Karima Kissa, Albert Sotto, Jean-Philippe Lavigne, Denis Costechareyre, Virginie Molle
Summary: Staphylococcus aureus is a virulent human pathogen with antibiotic resistance, making it a major public health threat. Bacteriophage therapy, a non-antibiotic strategy, shows potential in treating S. aureus infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Developmental Biology
Doris Lou Demy, Anne-Lou Touret, Mylene Lancino, Muriel Tauzin, Lavinia Capuana, Constance Pierre, Philippe Herbomel
Summary: Trim33 is a critical regulator in hematopoiesis, playing important roles in the production of red blood cells and macrophages. Loss of Trim33 leads to premature aging of macrophages and excessive production of neutrophils and thrombocytes.
Article
Biochemistry & Molecular Biology
Clara Taffoni, Johanna Marines, Hanane Chamma, Soumyabrata Guha, Mathilde Saccas, Amel Bouzid, Ana-Luiza Chaves Valadao, Clement Maghe, Jane Jardine, Mi Kyung Park, Katarzyna Polak, Mara De Martino, Claire Vanpouille-Box, Maguy Del Rio, Celine Gongora, Julie Gavard, Nicolas Bidere, Min Sup Song, Donovan Pineau, Jean-Philippe Hugnot, Karima Kissa, Laura Fontenille, Fabien P. Blanchet, Isabelle K. Vila, Nadine Laguette
Summary: Cytosolic DNA triggers inflammatory responses through the detection of cGAMP synthase (cGAS). It has been proposed that downregulation of cGAS is an immune escape strategy used by tumor cells. This study shows that in glioblastoma cells with low cGAS levels, the DNA-PK DNA repair complex can induce cGAS-independent immune responses and enhance cGAS-dependent signaling. The cooperation between DNA-PK and cGAS promotes the expression of chemokines that recruit macrophages, which impairs early tumorigenesis and is associated with poor outcome in glioblastoma patients.
Article
Cell Biology
Etienne Lelievre, Charlotte Bureau, Yann Bordat, Maxence Fretaud, Christelle Langevin, Chris Jopling, Karima Kissa
Summary: In this study, the zebrafish endoglin locus was characterized and it was found that deficiency of endoglin leads to heart failure and hypochromic anemia. This study also establishes the zebrafish as a valuable model for studying hereditary hemorrhagic telangiectasia (HHT).
DISEASE MODELS & MECHANISMS
(2023)
Article
Medicine, Research & Experimental
Laetitia Dard, Christophe Hubert, Pauline Esteves, Wendy Blanchard, Ghina Bou About, Lyla Baldasseroni, Elodie Dumon, Chloe Angelini, Megane Delourme, Veronique Guyonnet-Duperat, Stephane Claverol, Laura Fontenille, Karima Kissa, Pierre-Emmanuel Seguela, Jean-Benoit Thambo, Levy Nicolas, Yann Herault, Nadege Bellance, Nivea Dias Amoedo, Frederique Magdinier, Tania Sorg, Didier Lacombe, Rodrigue Rossignol
Summary: This study analyzed the molecular determinants of Costello syndrome and found that mitochondrial proteostasis and bioenergetics play a crucial role in the pathophysiology of the disease. The study suggests that patients with Costello syndrome may benefit from treatment with mitochondrial modulators.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
