Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES‐2145

Ten years of algal biofuel and bioproducts: gains and pains

(2019) Hui Chen et al.   PLANTA

CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1

(2019) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology for Biofuels

Highly efficient transgene‐free targeted mutagenesis and single‐stranded oligodeoxynucleotide‐mediated precise knock‐in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

(2019) Toshihisa Nomura et al.   PLANT BIOTECHNOLOGY JOURNAL

Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

(2018) Eric Poliner et al.   PLANT CELL REPORTS

Unrestrained markerless trait stacking inNannochloropsis gaditanathrough combined genome editing and marker recycling technologies

(2018) John Verruto et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779

(2018) Eric Poliner et al.   ACS Synthetic Biology

Lipid productivity in TALEN-induced starchless mutants of the unicellular green alga Coccomyxa sp. strain Obi

(2018) Keita Takahashi et al.   Algal Research-Biomass Biofuels and Bioproducts

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

(2018) Yuya Yoshimitsu et al.   Biotechnology for Biofuels

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

Targeting of Photoreceptor Genes inChlamydomonas reinhardtiivia Zinc-Finger Nucleases and CRISPR/Cas9

(2017) Andre Greiner et al.   PLANT CELL

Differently localized lysophosphatidic acid acyltransferases crucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis

(2017) Takashi Nobusawa et al.   PLANT JOURNAL

Metabolic engineering of Cyanobacteria and microalgae for enhanced production of biofuels and high-value products

(2016) M.A. Gomaa et al.   JOURNAL OF APPLIED MICROBIOLOGY

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9

(2016) Shigeru Makino et al.   Scientific Reports

Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii

(2015) Masako Iwai et al.   Frontiers in Microbiology

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits

(2014) Dongmei Wang et al.   PLoS Genetics

TALENoffer: genome-wide TALEN off-target prediction

(2013) Jan Grau et al.   BIOINFORMATICS

Efficient TALEN construction and evaluation methods for human cell and animal applications

(2013) Tetsushi Sakuma et al.   GENES TO CELLS

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

(2013) Tetsushi Sakuma et al.   Scientific Reports

TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction

(2012) Erin L. Doyle et al.   NUCLEIC ACIDS RESEARCH

Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

(2012) Randor Radakovits et al.   Nature Communications

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779

(2012) Astrid Vieler et al.   PLoS Genetics

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

An Outlook on Microalgal Biofuels

(2010) R. H. Wijffels et al.   SCIENCE

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

(2008) Qiang Hu et al.   PLANT JOURNAL