Rational design of thiolase substrate specificity for metabolic engineering applications

Engineering Erg10 Thiolase from Saccharomyces cerevisiae as a Synthetic Toolkit for the Production of Branched-Chain Alcohols

(2018) Pamela Torres-Salas et al.   BIOCHEMISTRY

Structural and Biochemical Studies of Substrate Selectivity in Ascaris suum Thiolases

(2018) Michael R. Blaisse et al.   BIOCHEMISTRY

Discovery and Engineering of Pathways for Production of α-Branched Organic Acids

(2017) Michael R. Blaisse et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Energy- and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions

(2016) Seokjung Cheong et al.   NATURE BIOTECHNOLOGY

Synthesis of medium-chain length (C6–C10) fuels and chemicals via β-oxidation reversal in Escherichia coli

(2015) Seohyoung Kim et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Coenzyme A-free activity, crystal structure, and rational engineering of a promiscuous β-ketoacyl thiolase from Ralstonia eutropha

(2015) Christopher D. Fage et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids

(2015) James M. Clomburg et al.   METABOLIC ENGINEERING

Acyl-Chain Elongation Drives Ketosynthase Substrate Selectivity intrans-Acyltransferase Polyketide Synthases

(2014) Matthew Jenner et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Crystal structure and biochemical characterization of beta-keto thiolase B from polyhydroxyalkanoate-producing bacterium Ralstonia eutropha H16

(2014) Eun-Jung Kim et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Comparative Analysis of the Substrate Specificity of trans- versus cis-Acyltransferases of Assembly Line Polyketide Synthases

(2014) Briana J. Dunn et al.   BIOCHEMISTRY

Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) and 3,4-dihydroxybutyric acid (3,4-DHBA) as value-added chemicals from glucose as a sole carbon source

(2014) Himanshu Dhamankar et al.   METABOLIC ENGINEERING

Retro-biosynthetic screening of a modular pathway design achieves selective route for microbial synthesis of 4-methyl-pentanol

(2014) Micah J. Sheppard et al.   Nature Communications

Functional Screening andIn VitroAnalysis Reveal Thioesterases with Enhanced Substrate Specificity Profiles That Improve Short-Chain Fatty Acid Production in Escherichia coli

(2013) Matthew D. McMahon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

(2013) Collin H. Martin et al.   Nature Communications

Thiolase engineering for enhanced butanol production inClostridium acetobutylicum

(2012) Miriam S. Mann et al.   BIOTECHNOLOGY AND BIOENGINEERING

Synthetic biology strategies for synthesizing polyhydroxyalkanoates from unrelated carbon sources

(2012) Daniel E. Agnew et al.   CHEMICAL ENGINEERING SCIENCE

Systems metabolic engineering of microorganisms for natural and non-natural chemicals

(2012) Jeong Wook Lee et al.   Nature Chemical Biology

Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways

(2012) H.-C. Tseng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Synthetic Biology Approach to Engineer a Functional Reversal of the β-Oxidation Cycle

(2012) James M. Clomburg et al.   ACS Synthetic Biology

Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyhexanoate) from Plant Oil by Engineered Ralstonia eutropha Strains

(2011) Charles F. Budde et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enzyme mechanism as a kinetic control element for designing synthetic biofuel pathways

(2011) Brooks B Bond-Watts et al.   Nature Chemical Biology

Metabolic Engineering of Escherichia coli for Enhanced Production of (R)- and (S)-3-Hydroxybutyrate

(2009) H.-C. Tseng et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Thiolase Reaction Mechanism: The Importance of Asn316 and His348 for Stabilizing the Enolate Intermediate of the Claisen Condensation

(2009) Gitte Meriläinen et al.   BIOCHEMISTRY

CHARMM: The biomolecular simulation program

(2009) B. R. Brooks et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme

(2008) Gitte Meriläinen et al.   FEBS Journal