Review
Chemistry, Analytical
Huyen Ngo, Sarnai Amartumur, Van Thi Ai Tran, Minh Tran, Yen N. Diep, Hansang Cho, Luke P. Lee
Summary: The advancements in cancer-on-chip technology have allowed for the recapitulation of vital biological features of various cancer types and their applications in life sciences and high-throughput drug screening. However, challenges such as the complexity of the tumor microenvironment and integrating multiple organs still need to be addressed. Integrated biosensors in advanced cancer-on-chip platforms offer high-throughput monitoring of real-time cellular responses for drug development.
Article
Chemistry, Multidisciplinary
Minghui Tang, Xin Duan, Anqi Yang, Shijie He, Yajing Zhou, Yuxin Liu, Lu Zhang, Xuan Luo, Peng Shi, Honglin Li, Xudong Lin
Summary: This study describes a fish capsule system that can be used for large-scale functional screening of fish, combined with real-time microscopy. This system allows for rapid screening and analysis of drugs, accelerating the discovery of new therapeutics.
Article
Chemistry, Analytical
Wenjie Jing, Shan Qiang, Zejun Jia, Qi Hao Shi, Xuan Meng, Mincong Yu, Hongyan Ma, Kai Zhao, Yujie Dai
Summary: Traditional methods for detecting organophosphorus pesticides are costly and complex, and also require expensive instruments. A nanozyme sensor array based on Ag2O nanospheres was developed to achieve simultaneous discrimination and efficient detection of a variety of OPs without the participation of natural enzymes. This work not only provides a convenient and high-efficiency method to identify OPs, but it also advances food safety and environmental monitoring.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Cell Biology
Marlene Geyer, Karla Queiroz
Summary: PDAC, the most common type of pancreatic cancer, is characterized by early metastasis and often requires chemotherapy treatment. With only 20% of tumors resectable, there is an urgent need for innovative modeling platforms to better understand and treat this complex disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Summary: Cellular phenotypes and functional responses are regulated by signals present in their microenvironment. High throughput culture platforms can better recapitulate and analyze these complex signals. Various techniques such as microplates, microarrays, and microfluidic cell culture systems have been developed for high throughput cell culture. Current applications include stem cell sourcing, drug discovery, and personalized medicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Environmental
L. Szydlowski, J. Ehlich, I. Goryanin, G. Pasternak
Summary: The study compared the electroactive capabilities of microbial communities from four mine drainages using a newly designed 96-well-plate array of microbial fuel cells. It demonstrated that the 96-well MFC array is a suitable platform for high-throughput screening, selection, and enrichment of electroactive consortia. Environmental conditions and physical and chemical parameters were found to be crucial for developing an efficient electroactive community.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemical Research Methods
Matthew Rienzo, Ke-Chih Lin, Kellen C. Mobilia, Eric K. Sackmann, Volker Kurz, Adam H. Navidi, Jarett King, Robert M. Onorato, Lawrence K. Chao, Tony Wu, Hanxiao Jiang, Justin K. Valley, Troy A. Lionberger, Michael D. Leavell
Summary: The high-throughput perfusion-based screening system allows real-time monitoring of microbial strain productivity to improve small molecule secretion efficiency. By applying whole-genome random mutagenesis, peak specific productivities of high-productivity strains can be substantially increased, leading to time savings in screening processes.
Article
Biotechnology & Applied Microbiology
Andrew E. S. Barentine, Yu Lin, Edward M. Courvan, Phylicia Kidd, Miao Liu, Leonhard Balduf, Timy Phan, Felix Rivera-Molina, Michael R. Grace, Zach Marin, Mark Lessard, Juliana Rios Chen, Siyuan Wang, Karla M. Neugebauer, Joerg Bewersdorf, David Baddeley
Summary: This study presents an integrated acquisition and analysis platform for single-molecule localization microscopy, which enables the imaging and analysis of 10,000 cells per day. The platform utilizes microscopy-specific data compression, distributed storage, and distributed analysis to increase throughput and allows for automated analysis initiation from the microscope. The platform also supports imaging of multiple cells in multi-well sample formats.
NATURE BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Kristin M. Bircsak, Richard DeBiasio, Mark Miedel, Alaa Alsebahi, Ryan Reddinger, Anthony Saleh, Tongying Shun, Lawrence A. Vernetti, Albert Gough
Summary: This study presents the development, automation, and validation of a 3D, microfluidic liver-on-a-chip system for high-throughput hepatotoxicity screening. The model consists of iPSC-derived hepatocyte aggregates co-cultured with endothelial cells and macrophages in a microchannel platform. With automation protocols and robust statistical analysis, the model demonstrated stable cell viability and functionality over time, making it a promising tool for hepatotoxicity screening and compound prioritization.
Review
Chemistry, Analytical
Yiling Sun, Ayelen Tayagui, Sarah Sale, Debolina Sarkar, Volker Nock, Ashley Garrill
Summary: Pathogenic fungi and oomycetes cause many animal and plant diseases, with emerging resistance to antifungal drugs posing challenges in treatment. High-throughput screening and lab-on-a-chip platforms offer potential for discovering new control strategies and drug targets. Specialized LOC platforms for force measurements and studying spore behaviors could aid future drug discovery efforts.
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Chemistry, Multidisciplinary
Matz Liebel, Irene Calderon, Nicolas Pazos-Perez, Niek F. van Hulst, Ramon A. Alvarez-Puebla
Summary: Large-scale SERS particle screening (LSSPS) is a fast multiplexed widefield screening approach that quantifies both Raman and Rayleigh scattering simultaneously, allowing for direct quantification of the fraction of SERS-active particles and unprecedented correlation of SERS activity with particle size.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biotechnology & Applied Microbiology
Suryong Kim, Jihoon Ko, Seung-Ryeol Lee, Dohyun Park, Seunghyuk Park, Noo Li Jeon
Summary: In vitro models are advancing, with organ-on-a-chip devices providing significant results but requiring further development. Anchor-IMPACT is a 3D anchor-based microfluidic injection-molded plastic array culture platform that enables selective 3D cell patterning for high-throughput angiogenesis evaluation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Xing Zhao, Gaozhi Ou, Mengcheng Lei, Yang Zhang, Lina Li, Anle Ge, Yachao Wang, Yiwei Li, Bi-Feng Liu
Summary: Cells are influenced by varying combinations of biochemical and mechanical cues in complex microenvironments. This study explores how biochemical cues interact with mechanical cues, by constructing a heterogeneous droplet/microgel array to analyze cell responses to different combinations of Wnt ligands and substrate stiffness. The device provides a powerful multiplexed bioassay platform for drug development, tissue engineering, and stem cell screening.
Article
Chemistry, Analytical
Le-He Jin, Yan Wei, Hui-Feng Wang, Jian-Bo Chen, Qun Fang
Summary: A simple method was developed for quantitatively metering nanoliter-scale liquids in parallel using a capillary array, achieving precise droplet generation with a relative standard deviation of 0.2%-3.5%. The system demonstrated high throughput screening capabilities and potential for large-scale analysis with different samples.
Article
Chemistry, Multidisciplinary
Yan-hang Wang, Hai-ning Lv, Qing-hua Cui, Peng-fei Tu, Yong Jiang, Ke-wu Zeng
ACTA PHARMACOLOGICA SINICA
(2020)
Article
Biochemistry & Molecular Biology
Yang Liu, Heng Yang, Qiang Guo, Tingting Liu, Yong Jiang, Mingbo Zhao, Kewu Zeng, Pengfei Tu
Article
Biochemistry & Molecular Biology
Yuemei Chen, Nankai Cao, Haining Lv, Kewu Zeng, Jingquan Yuan, Xiaoyu Guo, Mingbo Zhao, Pengfei Tu, Yong Jiang
Article
Biochemistry & Molecular Biology
Haizhen Liang, Nankai Cao, Kewu Zeng, Mingbo Zhao, Pengfei Tu, Yong Jiang
Article
Pharmacology & Pharmacy
Fujiang Wang, Ruiyan Li, Pengfei Tu, Jianping Chen, Kewu Zeng, Yong Jiang
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Xiaoni Ai, Yang Wu, Wenbo Lu, Xinran Zhang, Lin Zhao, Pengfei Tu, KeWei Wang, Yong Jiang
Article
Biochemistry & Molecular Biology
Nankai Cao, Sisi Zhu, Jun Li, Pengfei Tu, Yong Jiang
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
(2020)
Article
Plant Sciences
Nan-Kai Cao, Si-Si Zhu, Yue-Mei Chen, Xiao-Li Ma, Ming-Bo Zhao, Jun Li, Peng-Fei Tu, Yong Jiang
Summary: A new prenylated coumarin diglycoside and five known flavonoid glycosides were isolated from the leaves and stems of Clausena dunniana. These compounds were evaluated for their insulin-release promoting effects, with compounds 1, 2, and 4 exhibiting significant activities at 40 µM.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xiaoli Ma, Hongwei Chen, Sisi Zhu, Pengfei Tu, Yong Jiang
Summary: In this study, seventeen new carbazole alkaloid derivatives were isolated from the leaves and stems of Murraya microphylla, including trimeric and dimeric carbazole racemates. The structures of the new compounds were elucidated using HRESIMS and NMR data analysis, and the optically pure isomers were obtained through chiral HPLC separation.
Article
Plant Sciences
Mengqiu Lu, Zhiyong Du, Shuo Yuan, Qing Ma, Zhenzhou Han, Pengfei Tu, Yong Jiang
Summary: This study compared the effects of Murraya exotica L. and Murraya paniculata (L.) Jack on gastric lesions in rats and found that they have similar effects on improving gastric lesions and reducing inflammation and digestion related cytokine levels. Metabolomics results showed abnormalities in lipid, energy, and amino acid metabolism in rats with gastric lesions, and both plants demonstrated a similar metabolic modulation for gastric lesions. The study concluded that using both plants as the source plants of Murrayae Folium et Cacumen is feasible and reasonable for treating gastric lesions due to their similar pharmacodynamics and metabolic modulation ability. The combination of pharmacodynamics and metabolomics was also shown to be an efficient means for studying multi-source traditional Chinese medicine.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Si-Si Zhu, Yi-Fan Zhang, Meng Ding, Ke-Wu Zeng, Peng-Fei Tu, Yong Jiang
Summary: This study found that Clausena lenis Drake can be used as a traditional herbal medicine to treat various diseases, and identified the inhibitory activity of its compounds on BV-2 microglial cells, with the new compounds having significant effects on nitric oxide production.
Article
Biochemistry & Molecular Biology
Chao-Hua Li, Ying Zhou, Peng-Fei Tu, Ke-Wu Zeng, Yong Jiang
Summary: It was found that the carbazole alkaloid obtained from medicinal plants has a significant anti-neuroinflammation effect by directly targeting Sp1 and inhibiting NF-κB and MAPK signaling pathways.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Meng Fang, Yuqing Meng, Zhiyong Du, Mengqiu Guo, Yong Jiang, Pengfei Tu, Kun Hua, Yingyuan Lu, Xiaoyu Guo
Summary: The combination of Notoginseng and safflower shows a synergistic therapeutic effect on myocardial infarction by improving myocardial damage, apoptosis, oxidative stress, and inflammation. The study provides a theoretical basis for the clinical development of this combination.
Article
Biochemistry & Molecular Biology
Peng Gao, Kun Chang, Shuo Yuan, Yanhang Wang, Kewu Zeng, Yong Jiang, Pengfei Tu, Yingyuan Lu, Xiaoyu Guo
Summary: This study explored the mechanism and substance bases of Dictamnus dasycarpus Turcz hepatotoxicity using network pharmacology and molecular docking, verified through pharmacological experiments. The targets of DC included CYP1A2 and GSR, participating in various metabolic processes such as steroid metabolism, REDOX metabolism, and drug metabolism. In vitro and in vivo experiments confirmed that DC could induce hepatotoxicity by decreasing GSH-Px and inducing oxidative stress, as well as inhibiting the expression of CYP1A2.