Development of robust antibody purification by optimizing protein-A chromatography in combination with precipitation methodologies

A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies

(2014) Nabila Aboulaich et al.   BIOTECHNOLOGY PROGRESS

Multi-Faceted Arginine: Mechanism of the Effects of Arginine on Protein

(2014) Tsutomu Arakawa et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Development of a novel and efficient cell culture flocculation process using a stimulus responsive polymer to streamline antibody purification processes

(2013) Yun Kenneth Kang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing

(2013) Nicholas E. Levy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Asparagine Deamidation Dependence on Buffer Type, pH, and Temperature

(2013) Amanda L. Pace et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Host cell protein dynamics in recombinant CHO cells

(2013) Catherine EM Hogwood et al.   Bioengineered

Effect of Polysorbate 80 Concentration on Thermal and Photostability of a Monoclonal Antibody

(2012) Meera Agarkhed et al.   AAPS PHARMSCITECH

Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification

(2012) Yan Brodsky et al.   BIOTECHNOLOGY AND BIOENGINEERING

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

(2012) Catherine E.M. Hogwood et al.   BIOTECHNOLOGY AND BIOENGINEERING

Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography

(2012) Vikram N. Sisodiya et al.   Biotechnology Journal

Host cell protein adsorption characteristics during protein a chromatography

(2012) Richard D. R. Tarrant et al.   BIOTECHNOLOGY PROGRESS

Host cell protein dynamics in the supernatant of a mAb producing CHO cell line

(2011) A.S. Tait et al.   BIOTECHNOLOGY AND BIOENGINEERING

Select host cell proteins coelute with monoclonal antibodies in protein a chromatography

(2011) Bartek Nogal et al.   BIOTECHNOLOGY PROGRESS

Separation science is the key to successful biopharmaceuticals

(2011) Georges Guiochon et al.   JOURNAL OF CHROMATOGRAPHY A

Technology trends in antibody purification

(2011) Pete Gagnon   JOURNAL OF CHROMATOGRAPHY A

Arginine and the Hofmeister Series: The Role of Ion–Ion Interactions in Protein Aggregation Suppression

(2011) Curtiss P. Schneider et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Interaction of Arginine with Proteins and the Mechanism by Which It Inhibits Aggregation

(2010) Diwakar Shukla et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Recovery and purification process development for monoclonal antibody production

(2010) Hui F. Liu et al.   mAbs

A structural role for arginine in proteins: Multiple hydrogen bonds to backbone carbonyl oxygens

(2010) C.L. Borders et al.   PROTEIN SCIENCE

Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development

(2009) Mi Jin et al.   BIOTECHNOLOGY AND BIOENGINEERING

Suppression of Protein Aggregation by L-Arginine

(2009) Christian Lange et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Urea, but not guanidinium, destabilizes proteins by forming hydrogen bonds to the peptide group

(2009) Woon Ki Lim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Screening of effective column rinse solvent for Protein-A chromatography

(2009) Ryosuke Yumioka et al.   PROTEIN EXPRESSION AND PURIFICATION

High-throughput screening of chromatographic separations: II. Hydrophobic interaction

(2008) Jack F. Kramarczyk et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening of chromatographic separations: IV. Ion-exchange

(2008) Brian D. Kelley et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-throughput screening of chromatographic separations: I. Method development and column modeling

(2008) Jonathan L. Coffman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Host cell protein clearance during protein a chromatography: Development of an improved column wash step

(2008) Abhinav A. Shukla et al.   BIOTECHNOLOGY PROGRESS