Host cell protein adsorption characteristics during protein a chromatography

Host cell protein dynamics in the supernatant of a mAb producing CHO cell line

(2011) A.S. Tait et al.   BIOTECHNOLOGY AND BIOENGINEERING

Select host cell proteins coelute with monoclonal antibodies in protein a chromatography

(2011) Bartek Nogal et al.   BIOTECHNOLOGY PROGRESS

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

(2011) Meixia Zhou et al.   JOURNAL OF BIOTECHNOLOGY

Antibody capture by mixed-mode chromatography: A comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins

(2011) Jerome Pezzini et al.   JOURNAL OF CHROMATOGRAPHY A

Effects of solution environment on mammalian cell fermentation broth properties: Enhanced impurity removal and clarification performance

(2010) Matthew Westoby et al.   BIOTECHNOLOGY AND BIOENGINEERING

Fragmentation of a highly purified monoclonal antibody attributed to residual CHO cell protease activity

(2010) Sharon X. Gao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Monoclonal antibody purification using cationic polyelectrolytes: An alternative to column chromatography

(2010) Thanmaya Peram et al.   BIOTECHNOLOGY PROGRESS

Two-dimensional difference fluorescence gel electrophoresis to verify the scale-up of a non-affinity-based downstream process for isolation of a therapeutic recombinant antibody

(2010) Julita K. Grzeskowiak et al.   ELECTROPHORESIS

Potential aggregation prone regions in biotherapeutics

(2010) Xiaoling Wang et al.   mAbs

Recent advances in large-scale production of monoclonal antibodies and related proteins

(2010) Abhinav A. Shukla et al.   TRENDS IN BIOTECHNOLOGY

SELDI-TOF serum proteomics and colorectal cancer: A current overview

(2010) Timo Gemoll et al.   ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY

Strategies for analysing and improving the expression and quality of recombinant proteins made in mammalian cells

(2009) Nigel Jenkins et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Design of a filter train for precipitate removal in monoclonal antibody downstream processing

(2009) Sunitha Kandula et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Host cell proteins in biologics development: Identification, quantitation and risk assessment

(2009) Xing Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream process development

(2009) Mi Jin et al.   BIOTECHNOLOGY AND BIOENGINEERING

Integrated solution to purification challenges in the manufacture of a soluble recombinant protein inE. coli

(2009) Maire H. Caparon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Product and contaminant measurement in bioprocess development by SELDI-MS

(2009) Alex Berrill et al.   BIOTECHNOLOGY PROGRESS

Separation of product associatingE. colihost cell proteins OppA and DppA from recombinant apolipoprotein A-IMilanoin an industrial HIC unit operation

(2009) Alan K. Hunter et al.   BIOTECHNOLOGY PROGRESS

Review: Microscale methods for high-throughput chromatography development in the pharmaceutical industry

(2009) Sunil Chhatre et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Use of proteomics for design of a tailored host cell for highly efficient protein purification

(2009) Zhu Liu et al.   JOURNAL OF CHROMATOGRAPHY A

Screening of effective column rinse solvent for Protein-A chromatography

(2009) Ryosuke Yumioka et al.   PROTEIN EXPRESSION AND PURIFICATION

Proteins altered by elevated levels of palmitate or glucose implicated in impaired glucose-stimulated insulin secretion

(2009) E-ri M Sol et al.   Proteome Science

Selective antibody precipitation using polyelectrolytes: A novel approach to the purification of monoclonal antibodies

(2008) Paul McDonald et al.   BIOTECHNOLOGY AND BIOENGINEERING

Demonstration of Robust Host Cell Protein Clearance in Biopharmaceutical Downstream Processes

(2008) A.A. Shukla et al.   BIOTECHNOLOGY PROGRESS

Host cell protein clearance during protein a chromatography: Development of an improved column wash step

(2008) Abhinav A. Shukla et al.   BIOTECHNOLOGY PROGRESS

Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry

(2007) N. Kumar et al.   BIOTECHNOLOGY PROGRESS