Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis

Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli

(2019) Tian Tian et al.   ACS Synthetic Biology

Development of a CRISPR/Cas9 system for Methylococcus capsulatus in vivo gene editing

(2019) Timothy Tapscott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Reversible Gene Expression Control in Yersinia pestis by Using an Optimized CRISPR Interference System

(2019) Tong Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Establishment and application of CRISPR interference to affect sporulation, hydrogen peroxide detoxification, and mannitol catabolism in the methylotrophic thermophile Bacillus methanolicus

(2019) Kerstin Schultenkämper et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi

(2019) Henry H. Lee et al.   Nature Microbiology

Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi

(2019) Florence Caro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Broad-Host-Range CRISPRi Toolkit for Silencing Gene Expression in Burkholderia

(2019) Andrew M. Hogan et al.   ACS Synthetic Biology

Establishment and application of multiplexed CRISPR interference system in Bacillus licheniformis

(2019) Yangyang Zhan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Rapid identification of unknown carboxyl esterase activity in Corynebacterium glutamicum using RNA-guided CRISPR interference

(2018) Seung Soo Lee et al.   ENZYME AND MICROBIAL TECHNOLOGY

A Robust CRISPR Interference Gene Repression System in Pseudomonas

(2018) Sue Zanne Tan et al.   JOURNAL OF BACTERIOLOGY

Metabolic engineering to enhance heterologous production of hyaluronic acid in Bacillus subtilis

(2018) Adam W. Westbrook et al.   METABOLIC ENGINEERING

Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi)

(2018) Benjamin M. Woolston et al.   METABOLIC ENGINEERING

RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain

(2018) Jaehyun Park et al.   Microbial Cell Factories

OUP accepted manuscript

(2018)   NUCLEIC ACIDS RESEARCH

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

(2018) Tianmin Wang et al.   Nature Communications

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor

(2018) Jing Yang et al.   Microbial Cell Factories

Robust and stable transcriptional repression in Giardia using CRISPRi

(2018) S. G. McInally et al.   MOLECULAR BIOLOGY OF THE CELL

Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply

(2017) Wei-Fan Liang et al.   METABOLIC ENGINEERING

Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route

(2017) Yi-Ming Yang et al.   Microbial Cell Factories

Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi

(2017) Wei Tao et al.   Microbial Cell Factories

Genetic interaction mapping in mammalian cells using CRISPR interference

(2017) Dan Du et al.   NATURE METHODS

Replacing the Ethylmalonyl-CoA Pathway with the Glyoxylate Shunt Provides Metabolic Flexibility in the Central Carbon Metabolism of Methylobacterium extorquens AM1

(2017) Lennart Schada von Borzyskowski et al.   ACS Synthetic Biology

A transdisciplinary approach to the initial validation of a single cell protein as an alternative protein source for use in aquafeeds

(2017) Michael Tlusty et al.   PeerJ

Hopanoid-free Methylobacterium extorquens DM4 overproduces carotenoids and has widespread growth impairment

(2017) Alexander S. Bradley et al.   PLoS One

Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium

(2016) Mark R. Bruder et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of 2-Hydroxyisobutyric Acid from Methanol by Methylobacterium extorquens AM1 Expressing ( R )-3-Hydroxybutyryl Coenzyme A-Isomerizing Enzymes

(2016) Maria-Teresa Rohde et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance

(2016) Bo Hu et al.   Biotechnology for Biofuels

ProCarDB: a database of bacterial carotenoids

(2016) L. N. U. Nupur et al.   BMC MICROBIOLOGY

Selection Maintains Apparently Degenerate Metabolic Pathways due to Tradeoffs in Using Methylamine for Carbon versus Nitrogen

(2016) Dipti D. Nayak et al.   CURRENT BIOLOGY

Adding biotic complexity alters the metabolic benefits of mutualism

(2016) William R. Harcombe et al.   EVOLUTION

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB- co -4HB) biosynthesis

(2015) Li Lv et al.   METABOLIC ENGINEERING

Carotenoid Metabolism in Plants

(2015) Nazia Nisar et al.   Molecular Plant

Hopanoids as functional analogues of cholesterol in bacterial membranes

(2015) James P. Sáenz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

4-[2-O-11Z-Octadecenoyl-β-glucopyranosyl]-4,4′-diapolycopene-4,4′-dioic acid and 4-[2-O-9Z-hexadecenoyl-β-glucopyranosyl]-4,4′-diapolycopene-4,4′-dioic acid: new C30-carotenoids produced by Methylobacterium

(2015) Ayako Osawa et al.   TETRAHEDRON LETTERS

Gene silencing by CRISPR interference in mycobacteria

(2015) Eira Choudhary et al.   Nature Communications

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Sioxanthin, a novel glycosylated carotenoid, reveals an unusual subclustered biosynthetic pathway

(2014) Taylor K. S. Richter et al.   ENVIRONMENTAL MICROBIOLOGY

A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria

(2014) Lennart Schada von Borzyskowski et al.   ACS Synthetic Biology

Contribution of two ζ-carotene desaturases to the poly-cis desaturation pathway in the cyanobacterium Nostoc PCC 7120

(2013) Jürgen Breitenbach et al.   ARCHIVES OF MICROBIOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Optimization of Gene Expression through Divergent Mutational Paths

(2012) Hsin-Hung Chou et al.   Cell Reports

Prokaryotic gene clusters: A rich toolbox for synthetic biology

(2010) Michael Fischbach et al.   Biotechnology Journal

Methanol Assimilation in Methylobacterium extorquens AM1: Demonstration of All Enzymes and Their Regulation

(2010) Hana Šmejkalová et al.   PLoS One

Carotenoid biosynthesis in extremophilic Deinococcus–Thermus bacteria

(2010) Bing Tian et al.   TRENDS IN MICROBIOLOGY

Carotenoids of Gemmatimonas aurantiaca (Gemmatimonadetes): identification of a novel carotenoid, deoxyoscillol 2-rhamnoside, and proposed biosynthetic pathway of oscillol 2,2'-dirhamnoside

(2009) S. Takaichi et al.   MICROBIOLOGY-SGM

Methylobacterium Genome Sequences: A Reference Blueprint to Investigate Microbial Metabolism of C1 Compounds from Natural and Industrial Sources

(2009) Stéphane Vuilleumier et al.   PLoS One

Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation

(2009) Hsin-Hung Chou et al.   PLoS Genetics