Cyanobacterial Production of Biopharmaceutical and Biotherapeutic Proteins

Photosynthetic generation of heterologous terpenoids in cyanobacteria

(2019) Nico Betterle et al.   BIOTECHNOLOGY AND BIOENGINEERING

CRISPR/Cas9-mediated knockout of the RDR6 gene in Nicotiana benthamiana for efficient transient expression of recombinant proteins

(2019) Kouki Matsuo et al.   PLANTA

Genetic and metabolic advances in the engineering of cyanobacteria

(2019) Dhanya Vijay et al.   CURRENT OPINION IN BIOTECHNOLOGY

Biotechnology of cyanobacterial isoprene production

(2018) Julie E. Chaves et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound

(2018) Elias Englund et al.   METABOLIC ENGINEERING

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

(2018) Yuliya M. Dyo et al.   MICROBIOLOGY-SGM

Heterologous Leader Sequences in Fusion Constructs Enhance Expression of Geranyl Diphosphate Synthase and Yield of β-Phellandrene Production in Cyanobacteria (Synechocystis)

(2018) Nico Betterle et al.   ACS Synthetic Biology

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   ACS Synthetic Biology

Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures

(2017) Diego Hidalgo et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Engineering Isoprene Synthase Expression and Activity in Cyanobacteria

(2017) Julie E. Chaves et al.   ACS Synthetic Biology

Characterization of red-shifted phycobilisomes isolated from the chlorophyll f -containing cyanobacterium Halomicronema hongdechloris

(2016) Yaqiong Li et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Role of isopentenyl-diphosphate isomerase in heterologous cyanobacterial (Synechocystis) isoprene production

(2016) Julie E. Chaves et al.   PHOTOSYNTHESIS RESEARCH

Sustainable heterologous production of terpene hydrocarbons in cyanobacteria

(2016) Cinzia Formighieri et al.   PHOTOSYNTHESIS RESEARCH

A phycocyanin·phellandrene synthase fusion enhances recombinant protein expression and β-phellandrene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria)

(2015) Cinzia Formighieri et al.   METABOLIC ENGINEERING

Genetically engineering cyanobacteria to convert CO2, water, and light into the long-chain hydrocarbon farnesene

(2014) Charles Halfmann et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size

(2014) Henning Kirst et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Engineering cyanobacteria for the production of a cyclic hydrocarbon fuel from CO2 and H2O

(2014) Charles Halfmann et al.   GREEN CHEMISTRY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants

(2014) Cinzia Formighieri et al.   PLANTA

High yield soluble bacterial expression and streamlined purification of recombinant human interferon α-2a

(2014) Regina L. Bis et al.   PROTEIN EXPRESSION AND PURIFICATION

Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria

(2014) Jie Zhou et al.   Scientific Reports

Engineered Platform for Bioethylene Production by a Cyanobacterium Expressing a Chimeric Complex of Plant Enzymes

(2014) Sadanari Jindou et al.   ACS Synthetic Biology

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

(2013) James A. Gregory et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Paradigm of Monoterpene (β-phellandrene) Hydrocarbons Production via Photosynthesis in Cyanobacteria

(2013) Fiona K. Bentley et al.   BioEnergy Research

Heterologous Expression of the Mevalonic Acid Pathway in Cyanobacteria Enhances Endogenous Carbon Partitioning to Isoprene

(2013) Fiona K. Bentley et al.   Molecular Plant

Probing the consequences of antenna modification in cyanobacteria

(2013) Michelle Liberton et al.   PHOTOSYNTHESIS RESEARCH

Marker-free genetic engineering of the chloroplast in the green microalgaChlamydomonas reinhardtii

(2013) Hsu-Ching Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

Reduced light-harvesting antenna: Consequences on cyanobacterial metabolism and photosynthetic productivity

(2013) Jong-Hee Kwon et al.   Algal Research-Biomass Biofuels and Bioproducts

Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation

(2012) Lawrence E. Page et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Sustained photosynthetic conversion of CO2 to ethylene in recombinant cyanobacterium Synechocystis 6803

(2012) Justin Ungerer et al.   Energy & Environmental Science

A SingleN-Acetylgalactosamine Residue at Threonine 106 Modifies the Dynamics and Structure of Interferon α2a around the Glycosylation Site

(2012) Houman Ghasriani et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Steps toward a globally available malaria vaccine

(2012) Carla S. Jones et al.   Bioengineered

Diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms

(2011) Fiona K. Bentley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules

(2011) Sarah A. Wilson et al.   PLANT BIOTECHNOLOGY JOURNAL

Molecular Factors Affecting the Accumulation of Recombinant Proteins in the Chlamydomonas reinhardtii Chloroplast

(2010) Anna T. Coragliotti et al.   MOLECULAR BIOTECHNOLOGY

Structural organisation of phycobilisomes from Synechocystis sp. strain PCC6803 and their interaction with the membrane

(2009) Ana A. Arteni et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts

(2009) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

(2009) Pia Lindberg et al.   METABOLIC ENGINEERING

Interferon Gamma: Activity and ELISA Detection Comparisons

(2008) Sara Crisafulli et al.   BIOTECHNIQUES

Automated overexpression and isotopic labelling of biologically active oncoproteins in the cyanobacterium Anabaena sp. PCC 7120

(2008) Dominique Desplancq et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Exhaustion of the chloroplast protein synthesis capacity by massive expression of a highly stable protein antibiotic

(2008) Melanie Oey et al.   PLANT JOURNAL